Publicação
Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization
| Resumo: | A great demand for prebiotics is driving the search for new sources of fructo-oligosaccharides (FOS) producers and for FOS with differentiated functionalities. In the present work, FOS production by a new isolated strain of Aspergillus ibericus was evaluated. The temperature of fermentation and initial pH were optimized in shaken flask to yield a maximal FOS production, through a central composite experimental design. FOS were produced in a one-step bioprocess using the whole cells of the microorganism. The model (R2 = 0.918) predicted a yield of 0.56, experimentally 0.53 ± 0.03 gFOS.ginitial sucrose1 was obtained (37.0 °C and a pH of 6.2). A yield of 0.64 ± 0.02 gFOS.ginitial sucrose1 was obtained in the bioreactor, at 38 h, with a content of 118 ± 4 g.L1 in FOS and a purity of 56 ± 3%. The chemical structure of the FOS produced by A. ibericus was determined by HPLC and NMR. FOS were identified as 1-kestose, nystose, and 1F-fructofuranosylnystose. In conclusion, A. ibericus was found to be a good alternative FOS producer. |
|---|---|
| Autores principais: | Gonçalves, Clarisse Salomé Nobre |
| Outros Autores: | Alves Filho, E. G.; Fernandes, Fabiano A. N.; Brito, Edy S.; Rodrigues, S.; Teixeira, J. A.; Rodrigues, L. R. |
| Assunto: | Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866877028912857088 |
|---|---|
| author | Gonçalves, Clarisse Salomé Nobre |
| author2 | Alves Filho, E. G. Fernandes, Fabiano A. N. Brito, Edy S. Rodrigues, S. Teixeira, J. A. Rodrigues, L. R. |
| author2_role | author author author author author author |
| author_facet | Gonçalves, Clarisse Salomé Nobre Alves Filho, E. G. Fernandes, Fabiano A. N. Brito, Edy S. Rodrigues, S. Teixeira, J. A. Rodrigues, L. R. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Gonçalves, Clarisse Salomé Nobre\"},{\"Person.name\":\"Alves Filho, E. G.\"},{\"Person.name\":\"Fernandes, Fabiano A. N.\"},{\"Person.name\":\"Brito, Edy S.\"},{\"Person.name\":\"Rodrigues, S.\"},{\"Person.name\":\"Teixeira, J. A.\"},{\"Person.name\":\"Rodrigues, L. R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Gonçalves, Clarisse Salomé Nobre Alves Filho, E. G. Fernandes, Fabiano A. N. Brito, Edy S. Rodrigues, S. Teixeira, J. A. Rodrigues, L. R. |
| datacite.date.Accepted.fl_str_mv | 2018-03-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
| datacite.titles.title.fl_str_mv | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Gonçalves, Clarisse Salomé Nobre Alves Filho, E. G. Fernandes, Fabiano A. N. Brito, Edy S. Rodrigues, S. Teixeira, J. A. Rodrigues, L. R. |
| dc.date.Accepted.fl_str_mv | 2018-03-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/46766 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
| dc.title.fl_str_mv | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | A great demand for prebiotics is driving the search for new sources of fructo-oligosaccharides (FOS) producers and for FOS with differentiated functionalities. In the present work, FOS production by a new isolated strain of Aspergillus ibericus was evaluated. The temperature of fermentation and initial pH were optimized in shaken flask to yield a maximal FOS production, through a central composite experimental design. FOS were produced in a one-step bioprocess using the whole cells of the microorganism. The model (R2 = 0.918) predicted a yield of 0.56, experimentally 0.53 ± 0.03 gFOS.ginitial sucrose1 was obtained (37.0 °C and a pH of 6.2). A yield of 0.64 ± 0.02 gFOS.ginitial sucrose1 was obtained in the bioreactor, at 38 h, with a content of 118 ± 4 g.L1 in FOS and a purity of 56 ± 3%. The chemical structure of the FOS produced by A. ibericus was determined by HPLC and NMR. FOS were identified as 1-kestose, nystose, and 1F-fructofuranosylnystose. In conclusion, A. ibericus was found to be a good alternative FOS producer. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://prod-dspace.uminho.pt/bitstreams/62fd77d1-6bb5-4426-b322-d08171ee8e8b/download |
| id | rum_f5b216afcf86621d07ad19082bf64ac4 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/46766 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/46766 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Gonçalves, Clarisse Salomé Nobre Alves Filho, E. G. Fernandes, Fabiano A. N. Brito, Edy S. Rodrigues, S. Teixeira, J. A. Rodrigues, L. R. |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevierporA great demand for prebiotics is driving the search for new sources of fructo-oligosaccharides (FOS) producers and for FOS with differentiated functionalities. In the present work, FOS production by a new isolated strain of Aspergillus ibericus was evaluated. The temperature of fermentation and initial pH were optimized in shaken flask to yield a maximal FOS production, through a central composite experimental design. FOS were produced in a one-step bioprocess using the whole cells of the microorganism. The model (R2 = 0.918) predicted a yield of 0.56, experimentally 0.53 ± 0.03 gFOS.ginitial sucrose1 was obtained (37.0 °C and a pH of 6.2). A yield of 0.64 ± 0.02 gFOS.ginitial sucrose1 was obtained in the bioreactor, at 38 h, with a content of 118 ± 4 g.L1 in FOS and a purity of 56 ± 3%. The chemical structure of the FOS produced by A. ibericus was determined by HPLC and NMR. FOS were identified as 1-kestose, nystose, and 1F-fructofuranosylnystose. In conclusion, A. ibericus was found to be a good alternative FOS producer.application/pdfporProduction of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterizationGonçalves, Clarisse Salomé NobreAlves Filho, E. G.Fernandes, Fabiano A. N.Brito, Edy S.Rodrigues, S.Teixeira, J. A.Rodrigues, L. R.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0023-6438DOIIsPartOf10.1016/j.lwt.2017.10.0152018-032017-10-22T12:08:05Z2018-03-01T00:00:00ZHandlehttps://hdl.handle.net/1822/46766http://purl.org/coar/access_right/c_abf2open accessFructo-oligosaccharidesAspergillus ibericusChemical structureExperimental designProduction yield optimization551391 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://prod-dspace.uminho.pt/bitstreams/62fd77d1-6bb5-4426-b322-d08171ee8e8b/download |
| spellingShingle | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization Gonçalves, Clarisse Salomé Nobre Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
| status | SINGLETON |
| subject.fl_str_mv | Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
| title | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| title_full | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| title_fullStr | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| title_full_unstemmed | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| title_short | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| title_sort | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
| topic | Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
| topic_facet | Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
| url | https://hdl.handle.net/1822/46766 |
| visible | 1 |