Publication
Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community
| Summary: | H2 and CO2 are main compounds of synthesis gas. Efficient conversion of syngas to biomethane is a straightforward strategy to integrate the energy value of syngas into existing natural gas grid infrastructures. In this study, the effect of initial H2/CO2 (80/20, v/v) pressure on methane production rate and microbial community diversity was assessed in a hyperbaric bioreactor inoculated with anaerobic granular sludge. Several batch experiments were performed to distinguish between the effect of initial total gas pressure and H2/CO2 partial pressure: (1) varying initial gas pressure (from 1 to 6 bar) with 100% H2/CO2 mixture; (2) constant initial gas pressure (5 bar), with increasing H2/CO2 partial pressure (from 1 to 5 bar); (3) varying initial gas pressure (from 2 to 5 bar) with constant H2/CO2 partial pressure (2 bar). In (2) and (3), N2 was used for ensuring the necessary overpressure. Microbial community changes in the system were monitored by 16S rRNA-based techniques (PCR-DGGE). The raise of H2/CO2 initial pressure (100% H2/CO2) from 1 to 5 bar led to an improvement in methane rate production from 0.035 ± 0.014 mmol h1 to 0.072 ± 0.019 mmol h1. Similar methane production rates were observed in reactors operated at the same H2/CO2 partial pressures, even when varying the total initial gas pressure. Hydrogen partial pressure was shown to determine the structure of bacterial communities and diversity decreased with increasing H2/CO2 partial pressure. No significant changes were observed for the archaeal communities. |
|---|---|
| Main Authors: | Lopes, Marlene |
| Other Authors: | Alves, J. I.; Pereira, A.; Belo, Isabel; Sousa, D. Z.; Alves, M. M. |
| Year: | 2015 |
| Country: | Portugal |
| Document type: | other |
| Access type: | open access |
| Associated institution: | Universidade do Minho |
| Language: | English |
| Origin: | RepositóriUM - Universidade do Minho |
| _version_ | 1867438929107484672 |
|---|---|
| author | Lopes, Marlene |
| author2 | Alves, J. I. Pereira, A. Belo, Isabel Sousa, D. Z. Alves, M. M. |
| author2_role | author author author author author |
| author_facet | Lopes, Marlene Alves, J. I. Pereira, A. Belo, Isabel Sousa, D. Z. Alves, M. M. |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Lopes, Marlene\"},{\"Person.name\":\"Alves, J. I.\"},{\"Person.name\":\"Pereira, A.\"},{\"Person.name\":\"Belo, Isabel\"},{\"Person.name\":\"Sousa, D. Z.\"},{\"Person.name\":\"Alves, M. M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Lopes, Marlene Alves, J. I. Pereira, A. Belo, Isabel Sousa, D. Z. Alves, M. M. |
| datacite.date.Accepted.fl_str_mv | 2015-08-20T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-07-22T11:56:02Z |
| datacite.date.embargoed.fl_str_mv | 2015-07-22T11:56:02Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Lopes, Marlene Alves, J. I. Pereira, A. Belo, Isabel Sousa, D. Z. Alves, M. M. |
| dc.date.Accepted.fl_str_mv | 2015-08-20T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-07-22T11:56:02Z |
| dc.date.embargoed.fl_str_mv | 2015-07-22T11:56:02Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/36328 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier B.V. |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_1843 |
| description | H2 and CO2 are main compounds of synthesis gas. Efficient conversion of syngas to biomethane is a straightforward strategy to integrate the energy value of syngas into existing natural gas grid infrastructures. In this study, the effect of initial H2/CO2 (80/20, v/v) pressure on methane production rate and microbial community diversity was assessed in a hyperbaric bioreactor inoculated with anaerobic granular sludge. Several batch experiments were performed to distinguish between the effect of initial total gas pressure and H2/CO2 partial pressure: (1) varying initial gas pressure (from 1 to 6 bar) with 100% H2/CO2 mixture; (2) constant initial gas pressure (5 bar), with increasing H2/CO2 partial pressure (from 1 to 5 bar); (3) varying initial gas pressure (from 2 to 5 bar) with constant H2/CO2 partial pressure (2 bar). In (2) and (3), N2 was used for ensuring the necessary overpressure. Microbial community changes in the system were monitored by 16S rRNA-based techniques (PCR-DGGE). The raise of H2/CO2 initial pressure (100% H2/CO2) from 1 to 5 bar led to an improvement in methane rate production from 0.035 ± 0.014 mmol h1 to 0.072 ± 0.019 mmol h1. Similar methane production rates were observed in reactors operated at the same H2/CO2 partial pressures, even when varying the total initial gas pressure. Hydrogen partial pressure was shown to determine the structure of bacterial communities and diversity decreased with increasing H2/CO2 partial pressure. No significant changes were observed for the archaeal communities. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | other |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/e0351979-1e0d-4c10-b8ef-72b1865ecbc0/download |
| id | rum_f5fb4e12a4b010c1ca5fa35d54849f93 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/36328 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
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| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/36328 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Lopes, Marlene Alves, J. I. Pereira, A. Belo, Isabel Sousa, D. Z. Alves, M. M. |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Elsevier B.V. |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engElsevier B.V.porH2 and CO2 are main compounds of synthesis gas. Efficient conversion of syngas to biomethane is a straightforward strategy to integrate the energy value of syngas into existing natural gas grid infrastructures. In this study, the effect of initial H2/CO2 (80/20, v/v) pressure on methane production rate and microbial community diversity was assessed in a hyperbaric bioreactor inoculated with anaerobic granular sludge. Several batch experiments were performed to distinguish between the effect of initial total gas pressure and H2/CO2 partial pressure: (1) varying initial gas pressure (from 1 to 6 bar) with 100% H2/CO2 mixture; (2) constant initial gas pressure (5 bar), with increasing H2/CO2 partial pressure (from 1 to 5 bar); (3) varying initial gas pressure (from 2 to 5 bar) with constant H2/CO2 partial pressure (2 bar). In (2) and (3), N2 was used for ensuring the necessary overpressure. Microbial community changes in the system were monitored by 16S rRNA-based techniques (PCR-DGGE). The raise of H2/CO2 initial pressure (100% H2/CO2) from 1 to 5 bar led to an improvement in methane rate production from 0.035 ± 0.014 mmol h1 to 0.072 ± 0.019 mmol h1. Similar methane production rates were observed in reactors operated at the same H2/CO2 partial pressures, even when varying the total initial gas pressure. Hydrogen partial pressure was shown to determine the structure of bacterial communities and diversity decreased with increasing H2/CO2 partial pressure. No significant changes were observed for the archaeal communities.application/pdfporHydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial communityLopes, MarleneAlves, J. I.Pereira, A.Belo, IsabelSousa, D. Z.Alves, M. M.HostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0168-1656EISSNIsPartOf0168-1656DOIIsPartOf10.1016/j.jbiotec.2015.06.1702015-07-22T11:56:02Z2015-08-202015-07-20T13:56:06Z2015-08-20T00:00:00ZHandlehttps://hdl.handle.net/1822/36328http://purl.org/coar/access_right/c_abf2open access81341 bytesother research producthttp://purl.org/coar/resource_type/c_1843otherhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/e0351979-1e0d-4c10-b8ef-72b1865ecbc0/download |
| spellingShingle | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community Lopes, Marlene |
| status | SINGLETON |
| title | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| title_full | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| title_fullStr | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| title_full_unstemmed | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| title_short | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| title_sort | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
| url | https://hdl.handle.net/1822/36328 |
| visible | 1 |