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Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials

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Resumo:Contamination of food contact surfaces by microbes such as Salmonella is directly associated with substantial industry costs and severe foodborne disease outbreaks. Several approaches have been developed to control microbial attachment; one approach is the development of food contact materials incorporating antimicrobial compounds. In the present study, Salmonella enterica Enteritidis adhesion and biofilm formation on regular and triclosan-impregnated kitchen bench stones (silestones) were assessed, as was cellular viability within biofilms. Enumeration of adhered cells on granite, marble, stainless steel, and silestones revealed that all materials were prone to bacterial colonization (4 to 5 log CFU/cm2), and no significant effect of triclosan was found. Conversely, results concerning biofilm formation highlighted a possible bacteriostatic activity of triclosan; smaller amounts of Salmonella Enteritidis biofilms were formed on impregnated silestones, and significantly lower numbers of viable cells (1 × 105 to 1 × 106 CFU/cm2) were found in these biofilms than in those on the other materials (1 × 107 CFU/cm2). All surfaces tested failed to promote food safety, and careful utilization with appropriate sanitation of these surfaces is critical in food processing environments. Nevertheless, because of its bacteriostatic activity, triclosan incorporated into silestones confers some advantage for controlling microbial contamination.
Autores principais:Rodrigues, Diana Alexandra Ferreira
Outros Autores:Teixeira, P.; Oliveira, Rosário; Azeredo, Joana
Assunto:Bench cover materials Triclosan Salmonella enterica Enteritidis Biofilm formation Cellular viability
Ano:2011
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
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author Rodrigues, Diana Alexandra Ferreira
author2 Teixeira, P.
Oliveira, Rosário
Azeredo, Joana
author2_role author
author
author
author_facet Rodrigues, Diana Alexandra Ferreira
Teixeira, P.
Oliveira, Rosário
Azeredo, Joana
author_role author
contributor_name_str_mv RepositóriUM - Universidade do Minho
country_str PT
creators_json_txt [{\"Person.name\":\"Rodrigues, Diana Alexandra Ferreira\"},{\"Person.name\":\"Teixeira, P.\"},{\"Person.name\":\"Oliveira, Rosário\"},{\"Person.name\":\"Azeredo, Joana\"}]
datacite.contributors.contributor.contributorName.fl_str_mv RepositóriUM - Universidade do Minho
datacite.creators.creator.creatorName.fl_str_mv Rodrigues, Diana Alexandra Ferreira
Teixeira, P.
Oliveira, Rosário
Azeredo, Joana
datacite.date.Accepted.fl_str_mv 2011-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2013-11-04T15:33:09Z
datacite.date.embargoed.fl_str_mv 2013-11-04T15:33:09Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Bench cover materials
Triclosan
Salmonella enterica Enteritidis
Biofilm formation
Cellular viability
datacite.titles.title.fl_str_mv Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
dc.contributor.none.fl_str_mv RepositóriUM - Universidade do Minho
dc.creator.none.fl_str_mv Rodrigues, Diana Alexandra Ferreira
Teixeira, P.
Oliveira, Rosário
Azeredo, Joana
dc.date.Accepted.fl_str_mv 2011-01-01T00:00:00Z
dc.date.available.fl_str_mv 2013-11-04T15:33:09Z
dc.date.embargoed.fl_str_mv 2013-11-04T15:33:09Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv https://hdl.handle.net/1822/25953
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv International Association for Food Protection
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Bench cover materials
Triclosan
Salmonella enterica Enteritidis
Biofilm formation
Cellular viability
dc.title.fl_str_mv Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Contamination of food contact surfaces by microbes such as Salmonella is directly associated with substantial industry costs and severe foodborne disease outbreaks. Several approaches have been developed to control microbial attachment; one approach is the development of food contact materials incorporating antimicrobial compounds. In the present study, Salmonella enterica Enteritidis adhesion and biofilm formation on regular and triclosan-impregnated kitchen bench stones (silestones) were assessed, as was cellular viability within biofilms. Enumeration of adhered cells on granite, marble, stainless steel, and silestones revealed that all materials were prone to bacterial colonization (4 to 5 log CFU/cm2), and no significant effect of triclosan was found. Conversely, results concerning biofilm formation highlighted a possible bacteriostatic activity of triclosan; smaller amounts of Salmonella Enteritidis biofilms were formed on impregnated silestones, and significantly lower numbers of viable cells (1 × 105 to 1 × 106 CFU/cm2) were found in these biofilms than in those on the other materials (1 × 107 CFU/cm2). All surfaces tested failed to promote food safety, and careful utilization with appropriate sanitation of these surfaces is critical in food processing environments. Nevertheless, because of its bacteriostatic activity, triclosan incorporated into silestones confers some advantage for controlling microbial contamination.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://repositorium.uminho.pt/bitstreams/32f5a4cb-8baf-48a0-83b2-3d1434b5789b/download
id rum_f80a0edf88f106b09da5efc4e9d41865
identifier.url.fl_str_mv https://hdl.handle.net/1822/25953
instacron_str repositorium
institution Universidade do Minho
instname_str Universidade do Minho
language eng
network_acronym_str rum
network_name_str RepositóriUM - Universidade do Minho
oai_identifier_str oai:repositorium.uminho.pt:1822/25953
organization_str_mv urn:organizationAcronym:repositorium
person_str_mv Rodrigues, Diana Alexandra Ferreira
Teixeira, P.
Oliveira, Rosário
Azeredo, Joana
publishDate 2011
publisher.none.fl_str_mv International Association for Food Protection
reponame_str RepositóriUM - Universidade do Minho
repository_id_str urn:repositoryAcronym:rum
service_str_mv urn:repositoryAcronym:rum
spelling engInternational Association for Food ProtectionporContamination of food contact surfaces by microbes such as Salmonella is directly associated with substantial industry costs and severe foodborne disease outbreaks. Several approaches have been developed to control microbial attachment; one approach is the development of food contact materials incorporating antimicrobial compounds. In the present study, Salmonella enterica Enteritidis adhesion and biofilm formation on regular and triclosan-impregnated kitchen bench stones (silestones) were assessed, as was cellular viability within biofilms. Enumeration of adhered cells on granite, marble, stainless steel, and silestones revealed that all materials were prone to bacterial colonization (4 to 5 log CFU/cm2), and no significant effect of triclosan was found. Conversely, results concerning biofilm formation highlighted a possible bacteriostatic activity of triclosan; smaller amounts of Salmonella Enteritidis biofilms were formed on impregnated silestones, and significantly lower numbers of viable cells (1 × 105 to 1 × 106 CFU/cm2) were found in these biofilms than in those on the other materials (1 × 107 CFU/cm2). All surfaces tested failed to promote food safety, and careful utilization with appropriate sanitation of these surfaces is critical in food processing environments. Nevertheless, because of its bacteriostatic activity, triclosan incorporated into silestones confers some advantage for controlling microbial contamination.application/pdfporSalmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materialsRodrigues, Diana Alexandra FerreiraTeixeira, P.Oliveira, RosárioAzeredo, JoanaHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptPMID21219760ISSNIsPartOf0362-028XISSNIsPartOf1944-9097DOIIsPartOf10.4315/0362-028X.JFP-10-1672013-11-04T15:33:09Z20112011-01-01T00:00:00ZHandlehttps://hdl.handle.net/1822/25953http://purl.org/coar/access_right/c_abf2open accessBench cover materialsTriclosanSalmonella enterica EnteritidisBiofilm formationCellular viability233800 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/32f5a4cb-8baf-48a0-83b2-3d1434b5789b/download
spellingShingle Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
Rodrigues, Diana Alexandra Ferreira
Bench cover materials
Triclosan
Salmonella enterica Enteritidis
Biofilm formation
Cellular viability
status SINGLETON
subject.fl_str_mv Bench cover materials
Triclosan
Salmonella enterica Enteritidis
Biofilm formation
Cellular viability
title Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
title_full Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
title_fullStr Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
title_full_unstemmed Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
title_short Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
title_sort Salmonella enterica enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials
topic Bench cover materials
Triclosan
Salmonella enterica Enteritidis
Biofilm formation
Cellular viability
topic_facet Bench cover materials
Triclosan
Salmonella enterica Enteritidis
Biofilm formation
Cellular viability
url https://hdl.handle.net/1822/25953
visible 1