Publicação
The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect
| Resumo: | BACKGROUND: The aim of this work was to study the ability of two different wild type strains of the yeast Yarrowia lipolytica to grow on olive mill wastewater (OMW) and their potential to produce high-value products such as lipases. Factors that affect cellular growth and OMW degradation were studied, such as nitrogen supplementation, cells concentration and surfactant addition. RESULTS: Both strains, W29 and IMUFRJ 50682, were able to grow in OMW with 19 g L-1 of COD and approximately 800 mg L-1 of total phenols concentration. The strain W29 presented the highest potential for extracellular lipase production in OMW medium. Lipase productivity was improved by the medium supplementation with ammonium sulphate up to 6 g L-1, leading to 80% of COD degradation and 70% of total phenols reduction. The surfactant Tween 80 enhanced cell growth and COD degradation, but had a negative effect on lipase activity. CONCLUSIONS Y. lipolytica has a great potential for OMW valorisation by its use as culture medium for biomass and enzymes production. The operating conditions that favoured lipase production differ from the conditions that improve COD reduction. |
|---|---|
| Autores principais: | Lopes, Marlene |
| Outros Autores: | Araújo, Carla; Aguedo, Mário; Gomes, Nelma; Gonçalves, Cristiana; Teixeira, J. A.; Belo, Isabel |
| Assunto: | Olive mill wastewater Yarrowia lipolytica Lipase Fermentation |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1867439119951462400 |
|---|---|
| author | Lopes, Marlene |
| author2 | Araújo, Carla Aguedo, Mário Gomes, Nelma Gonçalves, Cristiana Teixeira, J. A. Belo, Isabel |
| author2_role | author author author author author author |
| author_facet | Lopes, Marlene Araújo, Carla Aguedo, Mário Gomes, Nelma Gonçalves, Cristiana Teixeira, J. A. Belo, Isabel |
| author_role | author |
| contributor_name_str_mv | RepositóriUM - Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Lopes, Marlene\"},{\"Person.name\":\"Araújo, Carla\"},{\"Person.name\":\"Aguedo, Mário\"},{\"Person.name\":\"Gomes, Nelma\"},{\"Person.name\":\"Gonçalves, Cristiana\"},{\"Person.name\":\"Teixeira, J. A.\"},{\"Person.name\":\"Belo, Isabel\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | RepositóriUM - Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Lopes, Marlene Araújo, Carla Aguedo, Mário Gomes, Nelma Gonçalves, Cristiana Teixeira, J. A. Belo, Isabel |
| datacite.date.Accepted.fl_str_mv | 2009-04-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2009-08-17T14:30:40Z |
| datacite.date.embargoed.fl_str_mv | 2009-08-17T14:30:40Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Olive mill wastewater Yarrowia lipolytica Lipase Fermentation |
| datacite.titles.title.fl_str_mv | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| dc.contributor.none.fl_str_mv | RepositóriUM - Universidade do Minho |
| dc.creator.none.fl_str_mv | Lopes, Marlene Araújo, Carla Aguedo, Mário Gomes, Nelma Gonçalves, Cristiana Teixeira, J. A. Belo, Isabel |
| dc.date.Accepted.fl_str_mv | 2009-04-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2009-08-17T14:30:40Z |
| dc.date.embargoed.fl_str_mv | 2009-08-17T14:30:40Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/9484 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Society of Chemical Industry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Olive mill wastewater Yarrowia lipolytica Lipase Fermentation |
| dc.title.fl_str_mv | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | BACKGROUND: The aim of this work was to study the ability of two different wild type strains of the yeast Yarrowia lipolytica to grow on olive mill wastewater (OMW) and their potential to produce high-value products such as lipases. Factors that affect cellular growth and OMW degradation were studied, such as nitrogen supplementation, cells concentration and surfactant addition. RESULTS: Both strains, W29 and IMUFRJ 50682, were able to grow in OMW with 19 g L-1 of COD and approximately 800 mg L-1 of total phenols concentration. The strain W29 presented the highest potential for extracellular lipase production in OMW medium. Lipase productivity was improved by the medium supplementation with ammonium sulphate up to 6 g L-1, leading to 80% of COD degradation and 70% of total phenols reduction. The surfactant Tween 80 enhanced cell growth and COD degradation, but had a negative effect on lipase activity. CONCLUSIONS Y. lipolytica has a great potential for OMW valorisation by its use as culture medium for biomass and enzymes production. The operating conditions that favoured lipase production differ from the conditions that improve COD reduction. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/e89d471f-53b5-43d4-976c-867627ef744e/download |
| id | rum_f8be08044c35920f39be4e6df2c0efe2 |
| identifier.url.fl_str_mv | https://hdl.handle.net/1822/9484 |
| instacron_str | repositorium |
| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/9484 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Lopes, Marlene Araújo, Carla Aguedo, Mário Gomes, Nelma Gonçalves, Cristiana Teixeira, J. A. Belo, Isabel |
| publishDate | 2009 |
| publisher.none.fl_str_mv | Society of Chemical Industry |
| reponame_str | RepositóriUM - Universidade do Minho |
| repository_id_str | urn:repositoryAcronym:rum |
| service_str_mv | urn:repositoryAcronym:rum |
| spelling | engSociety of Chemical IndustryporBACKGROUND: The aim of this work was to study the ability of two different wild type strains of the yeast Yarrowia lipolytica to grow on olive mill wastewater (OMW) and their potential to produce high-value products such as lipases. Factors that affect cellular growth and OMW degradation were studied, such as nitrogen supplementation, cells concentration and surfactant addition. RESULTS: Both strains, W29 and IMUFRJ 50682, were able to grow in OMW with 19 g L-1 of COD and approximately 800 mg L-1 of total phenols concentration. The strain W29 presented the highest potential for extracellular lipase production in OMW medium. Lipase productivity was improved by the medium supplementation with ammonium sulphate up to 6 g L-1, leading to 80% of COD degradation and 70% of total phenols reduction. The surfactant Tween 80 enhanced cell growth and COD degradation, but had a negative effect on lipase activity. CONCLUSIONS Y. lipolytica has a great potential for OMW valorisation by its use as culture medium for biomass and enzymes production. The operating conditions that favoured lipase production differ from the conditions that improve COD reduction.application/pdfporThe use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effectLopes, MarleneAraújo, CarlaAguedo, MárioGomes, NelmaGonçalves, CristianaTeixeira, J. A.Belo, IsabelHostingInstitutionOrganizationalRepositóriUM - Universidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptCITATION"Journal of Chemical Technology & Biotechnology". ISSN 0268-2575. 84:4 (Apr. 2009) 533-537.ISSNIsPartOf0268-2575DOIIsPartOf10.1002/jctb.20752009-08-17T14:30:40Z2009-042009-04-01T00:00:00ZHandlehttps://hdl.handle.net/1822/9484http://purl.org/coar/access_right/c_abf2open accessOlive mill wastewaterYarrowia lipolyticaLipaseFermentation148365 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorium.uminho.pt/bitstreams/e89d471f-53b5-43d4-976c-867627ef744e/download |
| spellingShingle | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect Lopes, Marlene Olive mill wastewater Yarrowia lipolytica Lipase Fermentation |
| status | SINGLETON |
| subject.fl_str_mv | Olive mill wastewater Yarrowia lipolytica Lipase Fermentation |
| title | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| title_full | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| title_fullStr | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| title_full_unstemmed | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| title_short | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| title_sort | The use of olive mill wastewater by wild type Yarrowia lipolytica strains : medium supplementation and surfactant presence effect |
| topic | Olive mill wastewater Yarrowia lipolytica Lipase Fermentation |
| topic_facet | Olive mill wastewater Yarrowia lipolytica Lipase Fermentation |
| url | https://hdl.handle.net/1822/9484 |
| visible | 1 |