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Effect of glucose oxidase incorporation in chitosan edible films properties

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Detalhes bibliográficos
Resumo:Demand for biodegradable and environmentally friendly packaging has draw attention into the development of biodegradable, polymer based edible packaging films. Edible films’ functionality can be enhanced by incorporating active substances such as biocatalysts. The main objective of this work was to evaluate the effect of GOx incorporation in the mechanical and barrier properties of chitosan edible films. Films were prepared by the casting method using 2% (w/v) chitosan with 25% (w/w) glycerol, containing 1.1% (v/v) of a glucose oxidase solution (0.02% (w/v)). Water vapor, oxygen and carbon dioxide permeabilities were evaluated and Fourier Transform InfraRed (FTIR) analysis was performed. The results of the mechanical tests showed that tensile strength and elongation-at-break values of chitosan films increased with the incorporation of glucose oxidase (from 9.6 to 10.6 MPa and from 65.5 to 71.6 %, respectively), however no significant (p<0.05) changes were observed. From permeability tests it was observed that the barrier properties of chitosan films were not affected by the enzyme incorporation. FTIR analysis showed a specific chemical interaction between functional groups of glucose oxidase and bioactive groups of chitosan. These results suggest that chitosan films with incorporated GOx could be used as active packaging as a strategy for extension of food’s shelf-life.
Autores principais:Lopes, M. I.
Outros Autores:Martins, J. T.; Fonseca, L. P.; Vicente, A. A.
Assunto:Edible film Chitosan Glucose oxidase Barrier properties Mechanical properties FTIR
Ano:2011
País:Portugal
Tipo de documento:comunicação em conferência
Tipo de acesso:acesso aberto
Instituição associada:Universidade do Minho
Idioma:inglês
Origem:RepositóriUM - Universidade do Minho
Descrição
Resumo:Demand for biodegradable and environmentally friendly packaging has draw attention into the development of biodegradable, polymer based edible packaging films. Edible films’ functionality can be enhanced by incorporating active substances such as biocatalysts. The main objective of this work was to evaluate the effect of GOx incorporation in the mechanical and barrier properties of chitosan edible films. Films were prepared by the casting method using 2% (w/v) chitosan with 25% (w/w) glycerol, containing 1.1% (v/v) of a glucose oxidase solution (0.02% (w/v)). Water vapor, oxygen and carbon dioxide permeabilities were evaluated and Fourier Transform InfraRed (FTIR) analysis was performed. The results of the mechanical tests showed that tensile strength and elongation-at-break values of chitosan films increased with the incorporation of glucose oxidase (from 9.6 to 10.6 MPa and from 65.5 to 71.6 %, respectively), however no significant (p<0.05) changes were observed. From permeability tests it was observed that the barrier properties of chitosan films were not affected by the enzyme incorporation. FTIR analysis showed a specific chemical interaction between functional groups of glucose oxidase and bioactive groups of chitosan. These results suggest that chitosan films with incorporated GOx could be used as active packaging as a strategy for extension of food’s shelf-life.