Publicação
Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo
| Resumo: | According to the World Health Organization (WHO), chronic inflammatory-related diseases represent the greatest threat to human health. Indeed, failure in the resolution of inflammation leads to serious pathological conditions, such as cardiovascular diseases, arthritis, cancer, diabetes, autoimmune diseases, and neurodegen erative disorders that are often associated with extremely high human suffering and societal and economic burdens. Despite the number and efficacy of available therapeutic agents have been increased, the serious side effects associated with some of them often create a very high risk/benefit ratio for patients. Therefore, herein, a drug delivery system was engineered to overcome important drawbacks of conventional therapies and to have a synergistic action with the incorporated drug. Indeed, it will have an added beneficial role in controlling inflammation. For that, sardine (Sardina pilchardus) roe was used as the lipidic source to produce bioactive li posomes, namely fishroesomes. These spherical vesicles with â 326 nm in size and a significant negative surface charge (â -31 mV) were able to encapsulate and control the release of the anti-inflammatory drug celecoxib. Moreover, fishroesomes were cytocompatible for different cell types (chondrocytes and macrophages), at con centrations in which they present anti-inflammatory properties. Importantly, fishroesomes were more effective in reducing pro-inflammatory mediators than the free drug. We also demonstrated that a single intra-articular injection of the fishroesomes encapsulating or not celecoxib in an experimental rat model of inflammatory arthritis was safe and more effective in controlling the pain and reducing the synovial inflammation compared to the free drug. Notably, as the celecoxib concentration in the sardine roe-derived liposomes was less than half of the amount of free drug, this study demonstrates the value of fishroesomes in counteracting inflammation. Therefore, the developed formulations may be considered a promising therapeutic option for inflammatory conditions. |
|---|---|
| Autores principais: | Guedes, Marta |
| Outros Autores: | Castro, Joana Isabel Martins Cosme Vieira; Lima, Ana Cláudia Fernandes; Gonçalves, Virgínia M. F.; Tiritan, Maria Elizabeth; Reis, R. L.; Ferreira, Helena Susana Costa Machado; Neves, N. M. |
| Assunto: | Celecoxib Fishroesomes Inflammatory conditions Intra-articular injection Synovial inflammation Sardine roe |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso embargado |
| Instituição associada: | Universidade do Minho |
| Idioma: | inglês |
| Origem: | RepositóriUM - Universidade do Minho |
| _version_ | 1866878337119420416 |
|---|---|
| author | Guedes, Marta |
| author2 | Castro, Joana Isabel Martins Cosme Vieira Lima, Ana Cláudia Fernandes Gonçalves, Virgínia M. F. Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
| author2_role | author author author author author author author |
| author_facet | Guedes, Marta Castro, Joana Isabel Martins Cosme Vieira Lima, Ana Cláudia Fernandes Gonçalves, Virgínia M. F. Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
| author_role | author |
| contributor_name_str_mv | Universidade do Minho |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Guedes, Marta\"},{\"Person.name\":\"Castro, Joana Isabel Martins Cosme Vieira\"},{\"Person.name\":\"Lima, Ana Cláudia Fernandes\"},{\"Person.name\":\"Gonçalves, Virgínia M. F.\"},{\"Person.name\":\"Tiritan, Maria Elizabeth\"},{\"Person.name\":\"Reis, R. L.\"},{\"Person.name\":\"Ferreira, Helena Susana Costa Machado\"},{\"Person.name\":\"Neves, N. M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Universidade do Minho |
| datacite.creators.creator.creatorName.fl_str_mv | Guedes, Marta Castro, Joana Isabel Martins Cosme Vieira Lima, Ana Cláudia Fernandes Gonçalves, Virgínia M. F. Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
| datacite.date.Accepted.fl_str_mv | 2025-02-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2027-02-01T00:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2027-02-01T00:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_f1cf |
| datacite.subjects.subject.fl_str_mv | Celecoxib Fishroesomes Inflammatory conditions Intra-articular injection Synovial inflammation Sardine roe |
| datacite.titles.title.fl_str_mv | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| dc.contributor.none.fl_str_mv | Universidade do Minho |
| dc.creator.none.fl_str_mv | Guedes, Marta Castro, Joana Isabel Martins Cosme Vieira Lima, Ana Cláudia Fernandes Gonçalves, Virgínia M. F. Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
| dc.date.Accepted.fl_str_mv | 2025-02-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2027-02-01T00:00:00Z |
| dc.date.embargoed.fl_str_mv | 2027-02-01T00:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | https://hdl.handle.net/1822/101361 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_f1cf |
| dc.rights.rights.copyright.fl_str_mv | embargoedAccess (2 Years) |
| dc.subject.none.fl_str_mv | Celecoxib Fishroesomes Inflammatory conditions Intra-articular injection Synovial inflammation Sardine roe |
| dc.title.fl_str_mv | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | According to the World Health Organization (WHO), chronic inflammatory-related diseases represent the greatest threat to human health. Indeed, failure in the resolution of inflammation leads to serious pathological conditions, such as cardiovascular diseases, arthritis, cancer, diabetes, autoimmune diseases, and neurodegen erative disorders that are often associated with extremely high human suffering and societal and economic burdens. Despite the number and efficacy of available therapeutic agents have been increased, the serious side effects associated with some of them often create a very high risk/benefit ratio for patients. Therefore, herein, a drug delivery system was engineered to overcome important drawbacks of conventional therapies and to have a synergistic action with the incorporated drug. Indeed, it will have an added beneficial role in controlling inflammation. For that, sardine (Sardina pilchardus) roe was used as the lipidic source to produce bioactive li posomes, namely fishroesomes. These spherical vesicles with â 326 nm in size and a significant negative surface charge (â -31 mV) were able to encapsulate and control the release of the anti-inflammatory drug celecoxib. Moreover, fishroesomes were cytocompatible for different cell types (chondrocytes and macrophages), at con centrations in which they present anti-inflammatory properties. Importantly, fishroesomes were more effective in reducing pro-inflammatory mediators than the free drug. We also demonstrated that a single intra-articular injection of the fishroesomes encapsulating or not celecoxib in an experimental rat model of inflammatory arthritis was safe and more effective in controlling the pain and reducing the synovial inflammation compared to the free drug. Notably, as the celecoxib concentration in the sardine roe-derived liposomes was less than half of the amount of free drug, this study demonstrates the value of fishroesomes in counteracting inflammation. Therefore, the developed formulations may be considered a promising therapeutic option for inflammatory conditions. |
| dirty | 0 |
| eu_rights_str_mv | embargoedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorium.uminho.pt/bitstreams/339988c4-015f-4a1a-9019-65a9c265daf7/download |
| funding.funder.alternateName_str_mv | other other |
| funding.funder.identifier_str_mv | urn:openaire:fct_________::FCT urn:openaire:fct_________::FCT |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia, I.P. Fundação para a Ciência e a Tecnologia, I.P. |
| funding.identifier_str_mv | UIDP/50026/2020 PINFRA/22190/2016 |
| funding.name_str_mv | Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático Concurso para Financiamento de Projetos de Infraestruturas de Investigação inseridas no Roteiro Nacional de Infraestruturas de Investigação de Interesse Estratégico |
| funding_str_mv | UIDP/50026/2020 https://hdl.handle.net/1822/97208 PINFRA/22190/2016 https://hdl.handle.net/1822/97818 |
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| identifier.url.fl_str_mv | https://hdl.handle.net/1822/101361 |
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| institution | Universidade do Minho |
| instname_str | Universidade do Minho |
| language | eng |
| network_acronym_str | rum |
| network_name_str | RepositóriUM - Universidade do Minho |
| oai_identifier_str | oai:repositorium.uminho.pt:1822/101361 |
| organization_str_mv | urn:organizationAcronym:repositorium |
| person_str_mv | Guedes, Marta Castro, Joana Isabel Martins Cosme Vieira Lima, Ana Cláudia Fernandes Gonçalves, Virgínia M. F. Tiritan, Maria Elizabeth Reis, R. L. Ferreira, Helena Susana Costa Machado Neves, N. M. |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | RepositóriUM - Universidade do Minho |
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| spelling | engElsevierengAccording to the World Health Organization (WHO), chronic inflammatory-related diseases represent the greatest threat to human health. Indeed, failure in the resolution of inflammation leads to serious pathological conditions, such as cardiovascular diseases, arthritis, cancer, diabetes, autoimmune diseases, and neurodegen erative disorders that are often associated with extremely high human suffering and societal and economic burdens. Despite the number and efficacy of available therapeutic agents have been increased, the serious side effects associated with some of them often create a very high risk/benefit ratio for patients. Therefore, herein, a drug delivery system was engineered to overcome important drawbacks of conventional therapies and to have a synergistic action with the incorporated drug. Indeed, it will have an added beneficial role in controlling inflammation. For that, sardine (Sardina pilchardus) roe was used as the lipidic source to produce bioactive li posomes, namely fishroesomes. These spherical vesicles with â 326 nm in size and a significant negative surface charge (â -31 mV) were able to encapsulate and control the release of the anti-inflammatory drug celecoxib. Moreover, fishroesomes were cytocompatible for different cell types (chondrocytes and macrophages), at con centrations in which they present anti-inflammatory properties. Importantly, fishroesomes were more effective in reducing pro-inflammatory mediators than the free drug. We also demonstrated that a single intra-articular injection of the fishroesomes encapsulating or not celecoxib in an experimental rat model of inflammatory arthritis was safe and more effective in controlling the pain and reducing the synovial inflammation compared to the free drug. Notably, as the celecoxib concentration in the sardine roe-derived liposomes was less than half of the amount of free drug, this study demonstrates the value of fishroesomes in counteracting inflammation. Therefore, the developed formulations may be considered a promising therapeutic option for inflammatory conditions.application/pdfengFishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivoGuedes, MartaCastro, Joana Isabel Martins Cosme VieiraLima, Ana Cláudia FernandesGonçalves, Virgínia M. F.Tiritan, Maria ElizabethReis, R. L.Ferreira, Helena Susana Costa MachadoNeves, N. M.HostingInstitutionOrganizationalUniversidade do Minhoe-mailmailto:repositorium@usdb.uminho.ptrepositorium@usdb.uminho.ptISSNIsPartOf0939-6411DOIIsPartOf10.1016/j.ejpb.2024.1145872025-022026-012026-04-27T14:01:51Z2025-02-01T00:00:00Z2027-02-01T00:00:00ZHandlehttps://hdl.handle.net/1822/101361http://purl.org/coar/access_right/c_f1cfembargoed accessCelecoxibFishroesomesInflammatory conditionsIntra-articular injectionSynovial inflammationSardine roe5883176 bytesFundação para a Ciência e a Tecnologia, I.P.ICVS/3B's - Associate Laboratory (UIDP/50026/2020)Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programáticohttps://hdl.handle.net/1822/97208UIDP/50026/2020Crossref Funder IDurn:openaire:fct_________::FCTFundação para a Ciência e a Tecnologia, I.P.Tissue Engineering and Regenerative Medicine Infrastructure (PINFRA/22190/2016)Concurso para Financiamento de Projetos de Infraestruturas de Investigação inseridas no Roteiro Nacional de Infraestruturas de Investigação de Interesse Estratégicohttps://hdl.handle.net/1822/97818PINFRA/22190/2016Crossref Funder IDurn:openaire:fct_________::FCTliteraturehttp://purl.org/coar/resource_type/c_6501journal article2025-02http://creativecommons.org/licenses/by/4.0/embargoedAccess (2 Years)http://purl.org/coar/access_right/c_f1cfapplication/pdffulltexthttps://repositorium.uminho.pt/bitstreams/339988c4-015f-4a1a-9019-65a9c265daf7/download207113 |
| spellingShingle | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo Guedes, Marta Celecoxib Fishroesomes Inflammatory conditions Intra-articular injection Synovial inflammation Sardine roe |
| status | SINGLETON |
| subject.fl_str_mv | Celecoxib Fishroesomes Inflammatory conditions Intra-articular injection Synovial inflammation Sardine roe |
| title | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| title_full | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| title_fullStr | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| title_full_unstemmed | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| title_short | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| title_sort | Fishroesomes show intrinsic anti-inflammatory bioactivity and ability as celecoxib carriers in vivo |
| topic | Celecoxib Fishroesomes Inflammatory conditions Intra-articular injection Synovial inflammation Sardine roe |
| topic_facet | Celecoxib Fishroesomes Inflammatory conditions Intra-articular injection Synovial inflammation Sardine roe |
| url | https://hdl.handle.net/1822/101361 |
| visible | 1 |
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