Publicação
Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila
| Resumo: | Neuron specification and maturation are essential for proper central nervous system development. However, the precise mechanisms that govern neuronal maturation, essential to shape and maintain neuronal circuitry, remain poorly understood. Here, we analyse early-born secondary neurons in the Drosophila larval brain, revealing that the early maturation of secondary neurons goes through 3 consecutive phases: (1) Immediately after birth, neurons express pan-neuronal markers but do not transcribe terminal differentiation genes; (2) Transcription of terminal differentiation genes, such as neurotransmitter-related genes VGlut, ChAT, or Gad1, starts shortly after neuron birth, but these transcripts are, however, not translated; (3) Translation of neurotransmitter-related genes only begins several hours later in mid-pupa stages in a coordinated manner with animal developmental stage, albeit in an ecdysone-independent manner. These results support a model where temporal regulation of transcription and translation of neurotransmitter-related genes is an important mechanism to coordinate neuron maturation with brain development. |
|---|---|
| Autores principais: | Marques, Graça S. |
| Outros Autores: | Teles-Reis, José; Konstantinides, Nikolaos; Brito, Patrícia H.; Homem, Catarina C.F.; Homem, Catarina C F |
| Assunto: | General Neuroscience General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Agricultural and Biological Sciences |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868414470155403264 |
|---|---|
| author | Marques, Graça S. |
| author2 | Teles-Reis, José Konstantinides, Nikolaos Brito, Patrícia H. Homem, Catarina C.F. Homem, Catarina C F |
| author2_role | author author author author author |
| author_facet | Marques, Graça S. Teles-Reis, José Konstantinides, Nikolaos Brito, Patrícia H. Homem, Catarina C.F. Homem, Catarina C F |
| author_role | author |
| contributor_name_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) iNOVA4Health - pólo NMS UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida Faculdade de Ciências e Tecnologia (FCT) Public Library of Science RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Marques, Graça S.\"},{\"Person.name\":\"Teles-Reis, José\"},{\"Person.name\":\"Konstantinides, Nikolaos\"},{\"Person.name\":\"Brito, Patrícia H.\"},{\"Person.name\":\"Homem, Catarina C.F.\"},{\"Person.name\":\"Homem, Catarina C F\",\"Person.identifier.orcid\":\"0000-0003-4243-0298\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) iNOVA4Health - pólo NMS UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida Faculdade de Ciências e Tecnologia (FCT) Public Library of Science RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Marques, Graça S. Teles-Reis, José Konstantinides, Nikolaos Brito, Patrícia H. Homem, Catarina C.F. Homem, Catarina C F |
| datacite.date.Accepted.fl_str_mv | 2023-05-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-06-15T22:20:41Z |
| datacite.date.embargoed.fl_str_mv | 2023-06-15T22:20:41Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | General Neuroscience General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Agricultural and Biological Sciences |
| datacite.titles.title.fl_str_mv | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| dc.contributor.none.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) iNOVA4Health - pólo NMS UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida Faculdade de Ciências e Tecnologia (FCT) Public Library of Science RUN |
| dc.creator.none.fl_str_mv | Marques, Graça S. Teles-Reis, José Konstantinides, Nikolaos Brito, Patrícia H. Homem, Catarina C.F. Homem, Catarina C F |
| dc.date.Accepted.fl_str_mv | 2023-05-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-06-15T22:20:41Z |
| dc.date.embargoed.fl_str_mv | 2023-06-15T22:20:41Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/153979 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | General Neuroscience General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Agricultural and Biological Sciences |
| dc.title.fl_str_mv | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Neuron specification and maturation are essential for proper central nervous system development. However, the precise mechanisms that govern neuronal maturation, essential to shape and maintain neuronal circuitry, remain poorly understood. Here, we analyse early-born secondary neurons in the Drosophila larval brain, revealing that the early maturation of secondary neurons goes through 3 consecutive phases: (1) Immediately after birth, neurons express pan-neuronal markers but do not transcribe terminal differentiation genes; (2) Transcription of terminal differentiation genes, such as neurotransmitter-related genes VGlut, ChAT, or Gad1, starts shortly after neuron birth, but these transcripts are, however, not translated; (3) Translation of neurotransmitter-related genes only begins several hours later in mid-pupa stages in a coordinated manner with animal developmental stage, albeit in an ecdysone-independent manner. These results support a model where temporal regulation of transcription and translation of neurotransmitter-related genes is an important mechanism to coordinate neuron maturation with brain development. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/3cbf57da-9b12-4624-a74f-62acb8242264/download |
| id | run_0e2fcac9052d58dc4aca959d76dc643d |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/153979 |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/153979 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Marques, Graça S. Teles-Reis, José Konstantinides, Nikolaos Brito, Patrícia H. Homem, Catarina C.F. Homem, Catarina C F Homem, Catarina C F http://orcid.org/0000-0003-4243-0298 0000-0003-4243-0298 |
| publishDate | 2023 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenNeuron specification and maturation are essential for proper central nervous system development. However, the precise mechanisms that govern neuronal maturation, essential to shape and maintain neuronal circuitry, remain poorly understood. Here, we analyse early-born secondary neurons in the Drosophila larval brain, revealing that the early maturation of secondary neurons goes through 3 consecutive phases: (1) Immediately after birth, neurons express pan-neuronal markers but do not transcribe terminal differentiation genes; (2) Transcription of terminal differentiation genes, such as neurotransmitter-related genes VGlut, ChAT, or Gad1, starts shortly after neuron birth, but these transcripts are, however, not translated; (3) Translation of neurotransmitter-related genes only begins several hours later in mid-pupa stages in a coordinated manner with animal developmental stage, albeit in an ecdysone-independent manner. These results support a model where temporal regulation of transcription and translation of neurotransmitter-related genes is an important mechanism to coordinate neuron maturation with brain development.application/pdfenAsynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in DrosophilaMarques, Graça S.Teles-Reis, JoséKonstantinides, NikolaosBrito, Patrícia H.Homem, Catarina C.F.PersonalHomem, Catarina C FDSpacehttp://dspace.org/items/d175310d-3f45-4446-a390-546a4d9a27caDSpacehttp://dspace.org/items/d175310d-3f45-4446-a390-546a4d9a27caHomemCatarinaORCIDhttp://orcid.org0000-0003-4243-0298NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)iNOVA4Health - pólo NMSUCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaFaculdade de Ciências e Tecnologia (FCT)Public Library of ScienceHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1545-7885URNIsPartOfPURE: 63726261URNIsPartOfPURE UUID: b396ca65-fc40-4c34-add3-2a49027fab32URNIsPartOfScopus: 85160967128URNIsPartOfPubMed: 37205703URNIsPartOfORCID: /0000-0002-2457-7520/work/151431817DOIIsPartOf10.1371/journal.pbio.30021152023-06-15T22:20:41Z2023-05-012023-05-01T00:00:00ZHandlehttp://hdl.handle.net/10362/153979http://purl.org/coar/access_right/c_abf2open accessGeneral NeuroscienceGeneral Biochemistry,Genetics and Molecular BiologyGeneral Immunology and MicrobiologyGeneral Agricultural and Biological Sciences3592193 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/3cbf57da-9b12-4624-a74f-62acb8242264/download |
| spellingShingle | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila Marques, Graça S. General Neuroscience General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Agricultural and Biological Sciences |
| status | SINGLETON |
| subject.fl_str_mv | General Neuroscience General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Agricultural and Biological Sciences |
| title | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| title_full | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| title_fullStr | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| title_full_unstemmed | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| title_short | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| title_sort | Asynchronous transcription and translation of neurotransmitter-related genes characterize the initial stages of neuronal maturation in Drosophila |
| topic | General Neuroscience General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Agricultural and Biological Sciences |
| topic_facet | General Neuroscience General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Agricultural and Biological Sciences |
| url | http://hdl.handle.net/10362/153979 |
| visible | 1 |