Publicação
PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration
| Resumo: | Therapeutic solutions for injuries in the peripheral nervous system are limited and not existing in the case of the central nervous system. The electrical stimulation of cells through a cell-supporting conductive scaffold may contribute to new therapeutic solutions for nerve regeneration. In this work, biocompatible Polylactic acid (PLA) fibrous scaffolds incorporating Fe(III)Tosylate (FeTos) were produced by electrospinning a mixture of PLA/FeTos solutions towards a rotating cylinder, inducing fiber alignment. Fibers were coated with the conductive polymer Poly(3,4 ethylenedioxythiophene) (PEDOT) formed by vapor-phase polymerization of EDOT at 70 °C for 2 h. Different solvents (ETH, DMF and THF) were used as FeTos solvents to investigate the impact on the scaffold’s conductivity. Scaffold conductivity was estimated to be as high as 1.50 × 10−1 S/cm when FeTos was dissolved in DMF. In vitro tests were performed to evaluate possible scaffold cytotoxicity, following ISO 10993-5, revealing no cytotoxic effects. Differentiation and growth of cells from the neural cell line SH-SY5Y seeded on the scaffolds were also assessed, with neuritic extensions observed in cells differentiated in neurons with retinoic acid. These extensions tended to follow the preferential alignment of the scaffold fibers. |
|---|---|
| Autores principais: | Pires, Laura S. |
| Outros Autores: | Melo, Diogo S.; Borges, João P.; Henriques, Célia R. |
| Assunto: | conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983114245603328 |
|---|---|
| author | Pires, Laura S. |
| author2 | Melo, Diogo S. Borges, João P. Henriques, Célia R. |
| author2_role | author author author |
| author_facet | Pires, Laura S. Melo, Diogo S. Borges, João P. Henriques, Célia R. |
| author_role | author |
| contributor_name_str_mv | DCM - Departamento de Ciência dos Materiais DF – Departamento de Física CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pires, Laura S.\"},{\"Person.name\":\"Melo, Diogo S.\"},{\"Person.name\":\"Borges, João P.\"},{\"Person.name\":\"Henriques, Célia R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | DCM - Departamento de Ciência dos Materiais DF – Departamento de Física CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Pires, Laura S. Melo, Diogo S. Borges, João P. Henriques, Célia R. |
| datacite.date.Accepted.fl_str_mv | 2023-10-05T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-01-24T17:02:20Z |
| datacite.date.embargoed.fl_str_mv | 2024-01-24T17:02:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production |
| datacite.titles.title.fl_str_mv | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| dc.contributor.none.fl_str_mv | DCM - Departamento de Ciência dos Materiais DF – Departamento de Física CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| dc.creator.none.fl_str_mv | Pires, Laura S. Melo, Diogo S. Borges, João P. Henriques, Célia R. |
| dc.date.Accepted.fl_str_mv | 2023-10-05T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-01-24T17:02:20Z |
| dc.date.embargoed.fl_str_mv | 2024-01-24T17:02:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/162711 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production |
| dc.title.fl_str_mv | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Therapeutic solutions for injuries in the peripheral nervous system are limited and not existing in the case of the central nervous system. The electrical stimulation of cells through a cell-supporting conductive scaffold may contribute to new therapeutic solutions for nerve regeneration. In this work, biocompatible Polylactic acid (PLA) fibrous scaffolds incorporating Fe(III)Tosylate (FeTos) were produced by electrospinning a mixture of PLA/FeTos solutions towards a rotating cylinder, inducing fiber alignment. Fibers were coated with the conductive polymer Poly(3,4 ethylenedioxythiophene) (PEDOT) formed by vapor-phase polymerization of EDOT at 70 °C for 2 h. Different solvents (ETH, DMF and THF) were used as FeTos solvents to investigate the impact on the scaffold’s conductivity. Scaffold conductivity was estimated to be as high as 1.50 × 10−1 S/cm when FeTos was dissolved in DMF. In vitro tests were performed to evaluate possible scaffold cytotoxicity, following ISO 10993-5, revealing no cytotoxic effects. Differentiation and growth of cells from the neural cell line SH-SY5Y seeded on the scaffolds were also assessed, with neuritic extensions observed in cells differentiated in neurons with retinoic acid. These extensions tended to follow the preferential alignment of the scaffold fibers. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/5333f12d-573c-4b96-8fcb-494ec15ec89f/download |
| id | run_2bdf3e76e08f69a831ced59a4397ca8f |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/162711 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/162711 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Pires, Laura S. Melo, Diogo S. Borges, João P. Henriques, Célia R. |
| publishDate | 2023 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
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| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenTherapeutic solutions for injuries in the peripheral nervous system are limited and not existing in the case of the central nervous system. The electrical stimulation of cells through a cell-supporting conductive scaffold may contribute to new therapeutic solutions for nerve regeneration. In this work, biocompatible Polylactic acid (PLA) fibrous scaffolds incorporating Fe(III)Tosylate (FeTos) were produced by electrospinning a mixture of PLA/FeTos solutions towards a rotating cylinder, inducing fiber alignment. Fibers were coated with the conductive polymer Poly(3,4 ethylenedioxythiophene) (PEDOT) formed by vapor-phase polymerization of EDOT at 70 °C for 2 h. Different solvents (ETH, DMF and THF) were used as FeTos solvents to investigate the impact on the scaffold’s conductivity. Scaffold conductivity was estimated to be as high as 1.50 × 10−1 S/cm when FeTos was dissolved in DMF. In vitro tests were performed to evaluate possible scaffold cytotoxicity, following ISO 10993-5, revealing no cytotoxic effects. Differentiation and growth of cells from the neural cell line SH-SY5Y seeded on the scaffolds were also assessed, with neuritic extensions observed in cells differentiated in neurons with retinoic acid. These extensions tended to follow the preferential alignment of the scaffold fibers.application/pdfenPEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural RegenerationPires, Laura S.Melo, Diogo S.Borges, João P.Henriques, Célia R.DCM - Departamento de Ciência dos MateriaisDF – Departamento de FísicaCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)MDPI - Multidisciplinary Digital Publishing InstituteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2073-4360URNIsPartOfPURE: 81996918URNIsPartOfPURE UUID: 4864f363-1744-4d01-8145-47d814483d7eURNIsPartOfScopus: 85173795280URNIsPartOfWOS: 001094695900001URNIsPartOfPubMed: 37836053URNIsPartOfPubMedCentral: PMC10575336URNIsPartOfORCID: /0000-0003-2711-1184/work/151389938URNIsPartOfORCID: /0000-0002-3996-6545/work/151413854DOIIsPartOf10.3390/polym151940042024-01-24T17:02:20Z2023-10-052023-10-05T00:00:00ZHandlehttp://hdl.handle.net/10362/162711http://purl.org/coar/access_right/c_abf2open accessconductivityelectrospinningFe(III)Tosylateneural regenerationPEDOTSH-SY5Y neuronal growthvapor-phase polymerizationGeneral ChemistryPolymers and PlasticsSDG 7 - Affordable and Clean EnergySDG 9 - Industry, Innovation, and InfrastructureSDG 12 - Responsible Consumption and Production4171853 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/5333f12d-573c-4b96-8fcb-494ec15ec89f/download |
| spellingShingle | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration Pires, Laura S. conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production |
| status | SINGLETON |
| subject.fl_str_mv | conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production |
| title | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| title_full | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| title_fullStr | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| title_full_unstemmed | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| title_short | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| title_sort | PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration |
| topic | conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production |
| topic_facet | conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production |
| url | http://hdl.handle.net/10362/162711 |
| visible | 1 |