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Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components

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Summary:Mössbauer spectroscopy was used to study the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743). Samples with different degrees of reduction were prepared using a redoxtitration technique. In the reduced cytochrome c3, all four hemes are reduced and exhibit diamagnetic Mössbauer spectra typical for low‐spin ferrous hemes (S= 0). In the oxidized protein, the hemes are low‐spin ferric (S= 1/2) and exhibit overlapping magnetic Mössbauer spectra. A method of differential spectroscopy was applied to deconvolute the four overlapping heme spectra and a crystal‐field model was used for data analysis. Characteristic Mössbauer spectral components for each heme group are obtained. Hyperfine and crystal‐field parameters for all four hemes are determined from these deconvoluted spectra.
Main Authors:Ravi, Natarajan
Other Authors:Moura, Isabel; Costa, Cristina; Teixeira, Miguel; LeGALL, Jean; Moura, José J. G.; HUYNH, Boi Hanh
Subject:Biochemistry
Year:1992
Country:Portugal
Document type:article
Access type:open access
Associated institution:Universidade Nova de Lisboa
Language:English
Origin:Repositório Institucional da UNL
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author Ravi, Natarajan
author2 Moura, Isabel
Costa, Cristina
Teixeira, Miguel
LeGALL, Jean
Moura, José J. G.
HUYNH, Boi Hanh
author2_role author
author
author
author
author
author
author_facet Ravi, Natarajan
Moura, Isabel
Costa, Cristina
Teixeira, Miguel
LeGALL, Jean
Moura, José J. G.
HUYNH, Boi Hanh
author_role author
contributor_name_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
Wiley-Blackwell
RUN
country_str PT
creators_json_txt [{\"Person.name\":\"Ravi, Natarajan\"},{\"Person.name\":\"Moura, Isabel\"},{\"Person.name\":\"Costa, Cristina\"},{\"Person.name\":\"Teixeira, Miguel\"},{\"Person.name\":\"LeGALL, Jean\"},{\"Person.name\":\"Moura, José J. G.\"},{\"Person.name\":\"HUYNH, Boi Hanh\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
Wiley-Blackwell
RUN
datacite.creators.creator.creatorName.fl_str_mv Ravi, Natarajan
Moura, Isabel
Costa, Cristina
Teixeira, Miguel
LeGALL, Jean
Moura, José J. G.
HUYNH, Boi Hanh
datacite.date.Accepted.fl_str_mv 1992-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2019-09-10T22:49:47Z
datacite.date.embargoed.fl_str_mv 2019-09-10T22:49:47Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Biochemistry
datacite.titles.title.fl_str_mv Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
Wiley-Blackwell
RUN
dc.creator.none.fl_str_mv Ravi, Natarajan
Moura, Isabel
Costa, Cristina
Teixeira, Miguel
LeGALL, Jean
Moura, José J. G.
HUYNH, Boi Hanh
dc.date.Accepted.fl_str_mv 1992-01-01T00:00:00Z
dc.date.available.fl_str_mv 2019-09-10T22:49:47Z
dc.date.embargoed.fl_str_mv 2019-09-10T22:49:47Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://www.scopus.com/inward/record.url?scp=0026610351&partnerID=8YFLogxK
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Biochemistry
dc.title.fl_str_mv Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Mössbauer spectroscopy was used to study the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743). Samples with different degrees of reduction were prepared using a redoxtitration technique. In the reduced cytochrome c3, all four hemes are reduced and exhibit diamagnetic Mössbauer spectra typical for low‐spin ferrous hemes (S= 0). In the oxidized protein, the hemes are low‐spin ferric (S= 1/2) and exhibit overlapping magnetic Mössbauer spectra. A method of differential spectroscopy was applied to deconvolute the four overlapping heme spectra and a crystal‐field model was used for data analysis. Characteristic Mössbauer spectral components for each heme group are obtained. Hyperfine and crystal‐field parameters for all four hemes are determined from these deconvoluted spectra.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://run.unl.pt/bitstreams/e6bee827-3968-410c-a0f3-e53448b77a0d/download
id run_31de4dc2bb95a75feb0333598ca026f0
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inst_facet_str urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa
instacron_str unl
institution Universidade Nova de Lisboa
instname_str Universidade Nova de Lisboa
language eng
network_acronym_str run
network_name_str Repositório Institucional da UNL
oai_identifier_str oai:run.unl.pt:10362/80773
organization_str_mv urn:organizationAcronym:unl
person_str_mv Ravi, Natarajan
Moura, Isabel
Costa, Cristina
Teixeira, Miguel
LeGALL, Jean
Moura, José J. G.
HUYNH, Boi Hanh
publishDate 1992
repo_facet_str urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL
reponame_str Repositório Institucional da UNL
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spelling engenMössbauer spectroscopy was used to study the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743). Samples with different degrees of reduction were prepared using a redoxtitration technique. In the reduced cytochrome c3, all four hemes are reduced and exhibit diamagnetic Mössbauer spectra typical for low‐spin ferrous hemes (S= 0). In the oxidized protein, the hemes are low‐spin ferric (S= 1/2) and exhibit overlapping magnetic Mössbauer spectra. A method of differential spectroscopy was applied to deconvolute the four overlapping heme spectra and a crystal‐field model was used for data analysis. Characteristic Mössbauer spectral components for each heme group are obtained. Hyperfine and crystal‐field parameters for all four hemes are determined from these deconvoluted spectra.application/pdfenMössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme componentsRavi, NatarajanMoura, IsabelCosta, CristinaTeixeira, MiguelLeGALL, JeanMoura, José J. G.HUYNH, Boi HanhInstituto de Tecnologia Química e Biológica António Xavier (ITQB)Wiley-BlackwellHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf0014-2956URNIsPartOfPURE: 14639635URNIsPartOfPURE UUID: de1a1d39-d3fb-4289-ac91-c64273ff780bURNIsPartOfScopus: 0026610351URNIsPartOfPubMed: 1311680URNIsPartOfWOS: A1992HH17300042URNIsPartOfORCID: /0000-0002-8611-9023/work/62320641URNIsPartOfORCID: /0000-0003-4124-6237/work/63725250URNIsPartOfORCID: /0000-0002-4726-2388/work/68772115DOIIsPartOf10.1111/j.1432-1033.1992.tb16694.x2019-09-10T22:49:47Z1992-01-011992-01-01T00:00:00ZURLhttp://www.scopus.com/inward/record.url?scp=0026610351&partnerID=8YFLogxKhttp://purl.org/coar/access_right/c_abf2open accessBiochemistry458431 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/e6bee827-3968-410c-a0f3-e53448b77a0d/download
spellingShingle Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
Ravi, Natarajan
Biochemistry
status SINGLETON
subject.fl_str_mv Biochemistry
title Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
title_full Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
title_fullStr Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
title_full_unstemmed Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
title_short Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
title_sort Mössbauer characterization of the tetraheme cytochrome c3 from Desulfovibrio baculatus (DSM 1743): Spectral deconvolution of the heme components
topic Biochemistry
topic_facet Biochemistry
url http://www.scopus.com/inward/record.url?scp=0026610351&partnerID=8YFLogxK
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