Publicação
Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells
| Resumo: | Given the growing interest in deep eutectic systems (DES) for their remarkable physicochemical and biological properties, and their alignment with green chemistry principles, we explored their therapeutic potential against colorectal cancer (CRC) in this study. The reported promising selective toxicity of therapeutic deep eutectic systems (THEDES), combining a terpene (menthol, thymol, perillyl alcohol and limonene) with ibuprofen (Ibu), towards CRC cells, have pushed them forward as potential alternative or complementary therapeutics towards CRC. These systems are considered THEDES since they are eutectic systems with a pharmaceutic ingredient as part of its composition. To leverage THEDES viability as CRC therapeutics, different drug delivery systems (DDS), based on poly (lactic-co-glycolic acid) nanoparticles (PLGA-NPs), liposomes (LPS) or niosomes, were explored to ensure THEDES accumulation in cancer sites. For that, nanoprecipitation, microfluidics and thin-film hydration methods were used, respectively, to produce THEDES encapsulated DDS. From the different DDS produced, LPS presented the most promising results regarding size (200 nm), monodispersity, and negative zeta potential averaging −30 mV. These results indicate LPS as a stable, monodispersed particle suspension suitable for cancer-related applications, hence, these NPs were selected for further assessments. The obtained THEDES-LPS revealed expected morphology upon electron microscopy observation (TEM), a 10 % release after 96h, and a maximum encapsulation efficiency (EE%) of 41 % obtained for limonene (Lim):Ibu (4:1). All THEDES-LPS revealed antiproliferative activity towards HT-29 cells which envisage LPS as a DDS for THEDES towards CRC. |
|---|---|
| Autores principais: | Oliveira, Filipe |
| Outros Autores: | Pereira, Joana; Neca, Carolina; Mansor, Muhamad Hawari; Gao, Zijian; Muthana, Munitta; Duarte, Ana Rita C. |
| Assunto: | Colorectal cancer Drug delivery systems Liposomes Nanoparticles Niosomes Polymeric nanoparticles Therapeutic deep eutectic systems Pharmaceutical Science SDG 3 - Good Health and Well-being |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868415488103546880 |
|---|---|
| author | Oliveira, Filipe |
| author2 | Pereira, Joana Neca, Carolina Mansor, Muhamad Hawari Gao, Zijian Muthana, Munitta Duarte, Ana Rita C. |
| author2_role | author author author author author author |
| author_facet | Oliveira, Filipe Pereira, Joana Neca, Carolina Mansor, Muhamad Hawari Gao, Zijian Muthana, Munitta Duarte, Ana Rita C. |
| author_role | author |
| contributor_name_str_mv | DQ - Departamento de Química LAQV@REQUIMTE Editions de Sante RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Oliveira, Filipe\"},{\"Person.name\":\"Pereira, Joana\"},{\"Person.name\":\"Neca, Carolina\"},{\"Person.name\":\"Mansor, Muhamad Hawari\"},{\"Person.name\":\"Gao, Zijian\"},{\"Person.name\":\"Muthana, Munitta\"},{\"Person.name\":\"Duarte, Ana Rita C.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | DQ - Departamento de Química LAQV@REQUIMTE Editions de Sante RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Oliveira, Filipe Pereira, Joana Neca, Carolina Mansor, Muhamad Hawari Gao, Zijian Muthana, Munitta Duarte, Ana Rita C. |
| datacite.date.Accepted.fl_str_mv | 2025-12-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-02-12T12:07:01Z |
| datacite.date.embargoed.fl_str_mv | 2026-02-12T12:07:01Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Colorectal cancer Drug delivery systems Liposomes Nanoparticles Niosomes Polymeric nanoparticles Therapeutic deep eutectic systems Pharmaceutical Science SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| dc.contributor.none.fl_str_mv | DQ - Departamento de Química LAQV@REQUIMTE Editions de Sante RUN |
| dc.creator.none.fl_str_mv | Oliveira, Filipe Pereira, Joana Neca, Carolina Mansor, Muhamad Hawari Gao, Zijian Muthana, Munitta Duarte, Ana Rita C. |
| dc.date.Accepted.fl_str_mv | 2025-12-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-02-12T12:07:01Z |
| dc.date.embargoed.fl_str_mv | 2026-02-12T12:07:01Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/200325 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Colorectal cancer Drug delivery systems Liposomes Nanoparticles Niosomes Polymeric nanoparticles Therapeutic deep eutectic systems Pharmaceutical Science SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Given the growing interest in deep eutectic systems (DES) for their remarkable physicochemical and biological properties, and their alignment with green chemistry principles, we explored their therapeutic potential against colorectal cancer (CRC) in this study. The reported promising selective toxicity of therapeutic deep eutectic systems (THEDES), combining a terpene (menthol, thymol, perillyl alcohol and limonene) with ibuprofen (Ibu), towards CRC cells, have pushed them forward as potential alternative or complementary therapeutics towards CRC. These systems are considered THEDES since they are eutectic systems with a pharmaceutic ingredient as part of its composition. To leverage THEDES viability as CRC therapeutics, different drug delivery systems (DDS), based on poly (lactic-co-glycolic acid) nanoparticles (PLGA-NPs), liposomes (LPS) or niosomes, were explored to ensure THEDES accumulation in cancer sites. For that, nanoprecipitation, microfluidics and thin-film hydration methods were used, respectively, to produce THEDES encapsulated DDS. From the different DDS produced, LPS presented the most promising results regarding size (200 nm), monodispersity, and negative zeta potential averaging −30 mV. These results indicate LPS as a stable, monodispersed particle suspension suitable for cancer-related applications, hence, these NPs were selected for further assessments. The obtained THEDES-LPS revealed expected morphology upon electron microscopy observation (TEM), a 10 % release after 96h, and a maximum encapsulation efficiency (EE%) of 41 % obtained for limonene (Lim):Ibu (4:1). All THEDES-LPS revealed antiproliferative activity towards HT-29 cells which envisage LPS as a DDS for THEDES towards CRC. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/19c0d92b-0055-4284-a051-5d01695ff8ae/download |
| id | run_320e17e050fa6042902c4aebbd737c71 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/200325 |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/200325 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Oliveira, Filipe Pereira, Joana Neca, Carolina Mansor, Muhamad Hawari Gao, Zijian Muthana, Munitta Duarte, Ana Rita C. |
| publishDate | 2025 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenGiven the growing interest in deep eutectic systems (DES) for their remarkable physicochemical and biological properties, and their alignment with green chemistry principles, we explored their therapeutic potential against colorectal cancer (CRC) in this study. The reported promising selective toxicity of therapeutic deep eutectic systems (THEDES), combining a terpene (menthol, thymol, perillyl alcohol and limonene) with ibuprofen (Ibu), towards CRC cells, have pushed them forward as potential alternative or complementary therapeutics towards CRC. These systems are considered THEDES since they are eutectic systems with a pharmaceutic ingredient as part of its composition. To leverage THEDES viability as CRC therapeutics, different drug delivery systems (DDS), based on poly (lactic-co-glycolic acid) nanoparticles (PLGA-NPs), liposomes (LPS) or niosomes, were explored to ensure THEDES accumulation in cancer sites. For that, nanoprecipitation, microfluidics and thin-film hydration methods were used, respectively, to produce THEDES encapsulated DDS. From the different DDS produced, LPS presented the most promising results regarding size (200 nm), monodispersity, and negative zeta potential averaging −30 mV. These results indicate LPS as a stable, monodispersed particle suspension suitable for cancer-related applications, hence, these NPs were selected for further assessments. The obtained THEDES-LPS revealed expected morphology upon electron microscopy observation (TEM), a 10 % release after 96h, and a maximum encapsulation efficiency (EE%) of 41 % obtained for limonene (Lim):Ibu (4:1). All THEDES-LPS revealed antiproliferative activity towards HT-29 cells which envisage LPS as a DDS for THEDES towards CRC.application/pdfenNanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cellsOliveira, FilipePereira, JoanaNeca, CarolinaMansor, Muhamad HawariGao, ZijianMuthana, MunittaDuarte, Ana Rita C.DQ - Departamento de QuímicaLAQV@REQUIMTEEditions de SanteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1773-2247URNIsPartOfPURE: 152910442URNIsPartOfPURE UUID: da56cc69-6d37-4860-a714-e04db2220599URNIsPartOfScopus: 105025707948URNIsPartOfWOS: 001583833000003URNIsPartOfORCID: /0000-0003-0800-0112/work/205572061DOIIsPartOf10.1016/j.jddst.2025.1075172026-02-12T12:07:01Z2025-122025-12-01T00:00:00ZHandlehttp://hdl.handle.net/10362/200325http://purl.org/coar/access_right/c_abf2open accessColorectal cancerDrug delivery systemsLiposomesNanoparticlesNiosomesPolymeric nanoparticlesTherapeutic deep eutectic systemsPharmaceutical ScienceSDG 3 - Good Health and Well-being63875 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/19c0d92b-0055-4284-a051-5d01695ff8ae/download |
| spellingShingle | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells Oliveira, Filipe Colorectal cancer Drug delivery systems Liposomes Nanoparticles Niosomes Polymeric nanoparticles Therapeutic deep eutectic systems Pharmaceutical Science SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | Colorectal cancer Drug delivery systems Liposomes Nanoparticles Niosomes Polymeric nanoparticles Therapeutic deep eutectic systems Pharmaceutical Science SDG 3 - Good Health and Well-being |
| title | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| title_full | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| title_fullStr | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| title_full_unstemmed | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| title_short | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| title_sort | Nanoparticle platforms for encapsulation of therapeutic deep eutectic systems to target colorectal cancer cells |
| topic | Colorectal cancer Drug delivery systems Liposomes Nanoparticles Niosomes Polymeric nanoparticles Therapeutic deep eutectic systems Pharmaceutical Science SDG 3 - Good Health and Well-being |
| topic_facet | Colorectal cancer Drug delivery systems Liposomes Nanoparticles Niosomes Polymeric nanoparticles Therapeutic deep eutectic systems Pharmaceutical Science SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/200325 |
| visible | 1 |