Publicação
With a little help from DNA barcoding
| Resumo: | The Gastropoda is one of the best studied classes of marine invertebrates. Yet, most species have been delimited based on morphology only. The application of DNA barcodes has shown to be greatly useful to help delimiting species. Therefore, sequences of the cytochrome c oxidase I gene from 108 specimens of 34 morpho-species were used to investigate the molecular diversity within the gastropods from the Portuguese coast. To the above dataset, we added available COI-5P sequences of taxonomically close species, in a total of 58 morpho-species examined. There was a good match between ours and sequences from independent studies, in public repositories. We found 32 concordant (91.4%) out of the 35 Barcode Index Numbers (BINs) generated from our sequences. The application of a ranking system to the barcodes yield over 70% with top taxonomic congruence, while 14.2% of the species barcodes had insufficient data. In the majority of the cases, there was a good concordance between morphological identification and DNA barcodes. Nonetheless, the discordance between morphological and molecular data is a reminder that even the comparatively well-known European marine gastropods can benefit from being probed using the DNA barcode approach. Discordant cases should be reviewed with more integrative studies. |
|---|---|
| Autores principais: | Borges, Luísa M. S. |
| Outros Autores: | Hollatz, Claudia; Lobo, Jorge; Cunha, Ana M.; Vilela, Ana P.; Calado, Gonçalo; Coelho, Rita; Costa, Ana C.; Ferreira, Maria S. G.; Costa, Maria H.; Costa, Filipe O. |
| Assunto: | cytochrome c oxidase General SDG 14 - Life Below Water |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1865920610792636416 |
|---|---|
| author | Borges, Luísa M. S. |
| author2 | Hollatz, Claudia Lobo, Jorge Cunha, Ana M. Vilela, Ana P. Calado, Gonçalo Coelho, Rita Costa, Ana C. Ferreira, Maria S. G. Costa, Maria H. Costa, Filipe O. |
| author2_role | author author author author author author author author author author |
| author_facet | Borges, Luísa M. S. Borges, Luísa M. S. Hollatz, Claudia Lobo, Jorge Cunha, Ana M. Vilela, Ana P. Calado, Gonçalo Coelho, Rita Costa, Ana C. Ferreira, Maria S. G. Costa, Maria H. Costa, Filipe O. Hollatz, Claudia Lobo, Jorge Cunha, Ana M. Vilela, Ana P. Calado, Gonçalo Coelho, Rita Costa, Ana C. Ferreira, Maria S. G. Costa, Maria H. Costa, Filipe O. |
| author_role | author |
| contributor_name_str_mv | DCEA - Departamento de Ciências e Engenharia do Ambiente Nature Publishing Group RUN |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Borges, Luísa M. S.\"},{\"Person.name\":\"Hollatz, Claudia\"},{\"Person.name\":\"Lobo, Jorge\"},{\"Person.name\":\"Cunha, Ana M.\"},{\"Person.name\":\"Vilela, Ana P.\"},{\"Person.name\":\"Calado, Gonçalo\"},{\"Person.name\":\"Coelho, Rita\"},{\"Person.name\":\"Costa, Ana C.\"},{\"Person.name\":\"Ferreira, Maria S. G.\"},{\"Person.name\":\"Costa, Maria H.\"},{\"Person.name\":\"Costa, Filipe O.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | DCEA - Departamento de Ciências e Engenharia do Ambiente Nature Publishing Group RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Borges, Luísa M. S. Hollatz, Claudia Lobo, Jorge Cunha, Ana M. Vilela, Ana P. Calado, Gonçalo Coelho, Rita Costa, Ana C. Ferreira, Maria S. G. Costa, Maria H. Costa, Filipe O. |
| datacite.date.Accepted.fl_str_mv | 2016-02-15T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-05-09T22:04:44Z |
| datacite.date.embargoed.fl_str_mv | 2018-05-09T22:04:44Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | cytochrome c oxidase General SDG 14 - Life Below Water |
| datacite.titles.title.fl_str_mv | With a little help from DNA barcoding Investigating the diversity of Gastropoda from the Portuguese coast |
| dc.contributor.none.fl_str_mv | DCEA - Departamento de Ciências e Engenharia do Ambiente Nature Publishing Group RUN |
| dc.creator.none.fl_str_mv | Borges, Luísa M. S. Hollatz, Claudia Lobo, Jorge Cunha, Ana M. Vilela, Ana P. Calado, Gonçalo Coelho, Rita Costa, Ana C. Ferreira, Maria S. G. Costa, Maria H. Costa, Filipe O. |
| dc.date.Accepted.fl_str_mv | 2016-02-15T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-05-09T22:04:44Z |
| dc.date.embargoed.fl_str_mv | 2018-05-09T22:04:44Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://www.scopus.com/inward/record.url?scp=84958280148&partnerID=8YFLogxK |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | cytochrome c oxidase General SDG 14 - Life Below Water |
| dc.title.fl_str_mv | With a little help from DNA barcoding Investigating the diversity of Gastropoda from the Portuguese coast |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The Gastropoda is one of the best studied classes of marine invertebrates. Yet, most species have been delimited based on morphology only. The application of DNA barcodes has shown to be greatly useful to help delimiting species. Therefore, sequences of the cytochrome c oxidase I gene from 108 specimens of 34 morpho-species were used to investigate the molecular diversity within the gastropods from the Portuguese coast. To the above dataset, we added available COI-5P sequences of taxonomically close species, in a total of 58 morpho-species examined. There was a good match between ours and sequences from independent studies, in public repositories. We found 32 concordant (91.4%) out of the 35 Barcode Index Numbers (BINs) generated from our sequences. The application of a ranking system to the barcodes yield over 70% with top taxonomic congruence, while 14.2% of the species barcodes had insufficient data. In the majority of the cases, there was a good concordance between morphological identification and DNA barcodes. Nonetheless, the discordance between morphological and molecular data is a reminder that even the comparatively well-known European marine gastropods can benefit from being probed using the DNA barcode approach. Discordant cases should be reviewed with more integrative studies. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/ac52a45a-0d45-461a-9c78-061d3dbe9fd5/download |
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| identifier.url.fl_str_mv | http://www.scopus.com/inward/record.url?scp=84958280148&partnerID=8YFLogxK |
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| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
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| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/36311 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Borges, Luísa M. S. Hollatz, Claudia Lobo, Jorge Cunha, Ana M. Vilela, Ana P. Calado, Gonçalo Coelho, Rita Costa, Ana C. Ferreira, Maria S. G. Costa, Maria H. Costa, Filipe O. |
| publishDate | 2016 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenThe Gastropoda is one of the best studied classes of marine invertebrates. Yet, most species have been delimited based on morphology only. The application of DNA barcodes has shown to be greatly useful to help delimiting species. Therefore, sequences of the cytochrome c oxidase I gene from 108 specimens of 34 morpho-species were used to investigate the molecular diversity within the gastropods from the Portuguese coast. To the above dataset, we added available COI-5P sequences of taxonomically close species, in a total of 58 morpho-species examined. There was a good match between ours and sequences from independent studies, in public repositories. We found 32 concordant (91.4%) out of the 35 Barcode Index Numbers (BINs) generated from our sequences. The application of a ranking system to the barcodes yield over 70% with top taxonomic congruence, while 14.2% of the species barcodes had insufficient data. In the majority of the cases, there was a good concordance between morphological identification and DNA barcodes. Nonetheless, the discordance between morphological and molecular data is a reminder that even the comparatively well-known European marine gastropods can benefit from being probed using the DNA barcode approach. Discordant cases should be reviewed with more integrative studies.application/pdfenWith a little help from DNA barcodingSubtitleenInvestigating the diversity of Gastropoda from the Portuguese coastBorges, Luísa M. S.Hollatz, ClaudiaLobo, JorgeCunha, Ana M.Vilela, Ana P.Calado, GonçaloCoelho, RitaCosta, Ana C.Ferreira, Maria S. G.Costa, Maria H.Costa, Filipe O.DCEA - Departamento de Ciências e Engenharia do AmbienteNature Publishing GroupHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptURNIsPartOfPURE: 1637622URNIsPartOfPURE UUID: d983a840-f580-4a03-a6ac-e8f643a1d8c8URNIsPartOfScopus: 84958280148URNIsPartOfWOS: 000370032400001URNIsPartOfPubMed: 26876495URNIsPartOfPubMedCentral: PMC4753432URNIsPartOfORCID: /0000-0001-8479-4941/work/50174003DOIIsPartOf10.1038/srep202262018-05-09T22:04:44Z2016-02-152016-02-15T00:00:00ZURLhttp://www.scopus.com/inward/record.url?scp=84958280148&partnerID=8YFLogxKhttp://purl.org/coar/access_right/c_abf2open accesscytochrome c oxidaseGeneralSDG 14 - Life Below Water1257394 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/ac52a45a-0d45-461a-9c78-061d3dbe9fd5/download |
| spellingShingle | With a little help from DNA barcoding With a little help from DNA barcoding Borges, Luísa M. S. cytochrome c oxidase General SDG 14 - Life Below Water Borges, Luísa M. S. cytochrome c oxidase General SDG 14 - Life Below Water |
| status | NEW |
| subject.fl_str_mv | cytochrome c oxidase General SDG 14 - Life Below Water |
| title | With a little help from DNA barcoding |
| title_full | With a little help from DNA barcoding |
| title_fullStr | With a little help from DNA barcoding With a little help from DNA barcoding |
| title_full_unstemmed | With a little help from DNA barcoding With a little help from DNA barcoding |
| title_short | With a little help from DNA barcoding |
| title_sort | With a little help from DNA barcoding |
| topic | cytochrome c oxidase General SDG 14 - Life Below Water |
| topic_facet | cytochrome c oxidase General SDG 14 - Life Below Water |
| url | http://www.scopus.com/inward/record.url?scp=84958280148&partnerID=8YFLogxK |
| visible | 1 |