Publicação
Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes
| Resumo: | The aim of this study was to explore the viability of the dual production of poly(3-hydroxybutyrate) (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved to screen for EPS-producing candidates and examine the impact of salinity and acetate on EPS synthesis. Salinity's known influence on EPS production and acetate's role in enhancing PHB production guided our parameter selection. Surprisingly, neither the introduction of an external carbon source (acetate) nor exposure to an abiotic stressor (salt) significantly altered EPS synthesis rates, which ranged from 25 to 150 mg·L−1, or its composition, with glucose being the dominant sugar component. Scaling up to a 3 L photobioreactor, we achieved simultaneous biopolymer production, reaching up to 205 mg·L−1 EPS and 87 mg·L−1 PHB. Additionally, the presence of uronic acid in the EPS facilitated biomass flocculation, streamlining the separation process, and potentially reducing associated time and costs. |
|---|---|
| Autores principais: | Altamira-Algarra, Beatriz |
| Outros Autores: | García, Joan; Torres, Cristiana A. V.; Reis, Maria A.M.; Gonzalez-Flo, Eva |
| Assunto: | Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983746424733696 |
|---|---|
| author | Altamira-Algarra, Beatriz |
| author2 | García, Joan Torres, Cristiana A. V. Reis, Maria A.M. Gonzalez-Flo, Eva |
| author2_role | author author author author |
| author_facet | Altamira-Algarra, Beatriz García, Joan Torres, Cristiana A. V. Reis, Maria A.M. Gonzalez-Flo, Eva |
| author_role | author |
| contributor_name_str_mv | UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química Elsevier RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Altamira-Algarra, Beatriz\"},{\"Person.name\":\"García, Joan\"},{\"Person.name\":\"Torres, Cristiana A. V.\"},{\"Person.name\":\"Reis, Maria A.M.\"},{\"Person.name\":\"Gonzalez-Flo, Eva\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química Elsevier RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Altamira-Algarra, Beatriz García, Joan Torres, Cristiana A. V. Reis, Maria A.M. Gonzalez-Flo, Eva |
| datacite.date.Accepted.fl_str_mv | 2025-07-25T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-06-03T21:18:57Z |
| datacite.date.embargoed.fl_str_mv | 2025-06-03T21:18:57Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology |
| datacite.titles.title.fl_str_mv | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| dc.contributor.none.fl_str_mv | UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química Elsevier RUN |
| dc.creator.none.fl_str_mv | Altamira-Algarra, Beatriz García, Joan Torres, Cristiana A. V. Reis, Maria A.M. Gonzalez-Flo, Eva |
| dc.date.Accepted.fl_str_mv | 2025-07-25T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-06-03T21:18:57Z |
| dc.date.embargoed.fl_str_mv | 2025-06-03T21:18:57Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/183808 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology |
| dc.title.fl_str_mv | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The aim of this study was to explore the viability of the dual production of poly(3-hydroxybutyrate) (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved to screen for EPS-producing candidates and examine the impact of salinity and acetate on EPS synthesis. Salinity's known influence on EPS production and acetate's role in enhancing PHB production guided our parameter selection. Surprisingly, neither the introduction of an external carbon source (acetate) nor exposure to an abiotic stressor (salt) significantly altered EPS synthesis rates, which ranged from 25 to 150 mg·L−1, or its composition, with glucose being the dominant sugar component. Scaling up to a 3 L photobioreactor, we achieved simultaneous biopolymer production, reaching up to 205 mg·L−1 EPS and 87 mg·L−1 PHB. Additionally, the presence of uronic acid in the EPS facilitated biomass flocculation, streamlining the separation process, and potentially reducing associated time and costs. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/fb1f28f2-8631-465d-83f4-a169727f8482/download |
| funder_facet_str_mv | EC{{{_:::_}}}European Commission FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia |
| funding.funder.alternateName_str_mv | EC FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100008530 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | European Commission Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | H2020 6817 - DCRRNI ID 6817 - DCRRNI ID |
| id | run_4d97063a7de2da8a5fa6ccd949bf57b0 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/183808 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/183808 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Altamira-Algarra, Beatriz García, Joan Torres, Cristiana A. V. Reis, Maria A.M. Gonzalez-Flo, Eva |
| publishDate | 2025 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenThe aim of this study was to explore the viability of the dual production of poly(3-hydroxybutyrate) (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved to screen for EPS-producing candidates and examine the impact of salinity and acetate on EPS synthesis. Salinity's known influence on EPS production and acetate's role in enhancing PHB production guided our parameter selection. Surprisingly, neither the introduction of an external carbon source (acetate) nor exposure to an abiotic stressor (salt) significantly altered EPS synthesis rates, which ranged from 25 to 150 mg·L−1, or its composition, with glucose being the dominant sugar component. Scaling up to a 3 L photobioreactor, we achieved simultaneous biopolymer production, reaching up to 205 mg·L−1 EPS and 87 mg·L−1 PHB. Additionally, the presence of uronic acid in the EPS facilitated biomass flocculation, streamlining the separation process, and potentially reducing associated time and costs.application/pdfenExploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomesAltamira-Algarra, BeatrizGarcía, JoanTorres, Cristiana A. V.Reis, Maria A.M.Gonzalez-Flo, EvaUCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaElsevierHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1871-6784URNIsPartOfPURE: 117434877URNIsPartOfPURE UUID: 99f2c7cb-dc27-4193-9ebc-6224586bb222URNIsPartOfScopus: 86000734224URNIsPartOfPubMed: 40081759URNIsPartOfWOS: 001486752300001DOIIsPartOf10.1016/j.nbt.2025.02.0082025-06-03T21:18:57Z2025-07-252025-07-25T00:00:00ZHandlehttp://hdl.handle.net/10362/183808http://purl.org/coar/access_right/c_abf2open accessBiopolymerBioprocess. Confocal Scanning Laser MicroscopePhotobioreactorPolyhydroxyalkanoateSynechoccocus sp.Synechocystis sp.BiotechnologyBioengineeringMolecular Biology1472521 bytesEuropean CommissionHARNESSING THE POWER OF NATURE THROUGH PRODUCTIVE MICROBIAL CONSORTIA IN BIOTECHNOLOGY – MEASURE, MODEL, MASTERH2020Crossref Funder IDhttp://doi.org/10.13039/501100008530Fundação para a Ciência e a TecnologiaApplied Molecular Biosciences Unit6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaApplied Molecular Biosciences Unit6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/fb1f28f2-8631-465d-83f4-a169727f8482/download |
| spellingShingle | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes Altamira-Algarra, Beatriz Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology |
| status | SINGLETON |
| subject.fl_str_mv | Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology |
| title | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| title_full | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| title_fullStr | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| title_full_unstemmed | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| title_short | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| title_sort | Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes |
| topic | Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology |
| topic_facet | Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology |
| url | http://hdl.handle.net/10362/183808 |
| visible | 1 |