Publicação
Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media
| Resumo: | Haemophilus influenzae is a commensal microorganism of the human nasopharynx, responsible for both invasive and non-invasive diseases. This work focused on the study of 93 H. influenzae isolates, collected between 2013 and 2018, from patients with two epidemiologically relevant non-invasive diseases: Chronic Obstructive Pulmonary Disease (COPD) and Otitis Media (OM). Phenotypical and molecular characterizations were performed for the isolates, regarding capsular typing, β-lactamase production, antibiotic susceptibility, genetic diversity by MLST, presence/absence of virulence factors (pilA, hifA, hmw1A, hmw2A, hia and ompP5) and ability to produce biofilms. COPD isolates were collected from adults (100%), while 98.2% of OM isolates were collected from children. Non-typeable H. influenzae (NTHi) was the aetiological agent in COPD (97.4%) and OM (100%). Regarding antibiotic susceptibility, it should be noticed that 15.1% of the isolates were β- lactamase producers. MLST revealed a high genetic diversity among COPD and OM isolates, with 31 STs in 41 analysed isolates. pilA and ompP5 genes were present in more than 50% of COPD and OM isolates. hifA and hia genes were identified in less than half of the isolates, with a higher prevalence of these among OM isolates. hmw1A and hmw2A genes were respectively identified in 25.5% and 32.7% of OM isolates, while both hmw genes were present in 76.3% of COPD isolates. Biofilm production was observed for 14.0% and 29.0% of all isolates after 24h and 48h, respectively. No relationship between biofilm production and clinical source could be established, as well as with the presence of virulence factors (pilA, hmw1A e hmw2A) involved in biofilm production. COPD and OM are frequently associated with NTHi. Since no vaccines are available, monitoring of these diseases is highly recommended, as these constitute a Public Health threat associated with a high economic and social burden. |
|---|---|
| Autores principais: | Batista, Beatriz da Cunha |
| Assunto: | Haemophilus influenzae COPD OM virulence factors biofilms NTHi |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1865920612039393280 |
|---|---|
| author | Batista, Beatriz da Cunha |
| author_facet | Batista, Beatriz da Cunha Batista, Beatriz da Cunha |
| author_role | author |
| contributor_name_str_mv | Lavado, Maria Paula Jordão, Maria Luísa RUN |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Batista, Beatriz da Cunha\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Lavado, Maria Paula Jordão, Maria Luísa RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Batista, Beatriz da Cunha |
| datacite.date.Accepted.fl_str_mv | 2018-10-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-01-21T11:33:48Z |
| datacite.date.embargoed.fl_str_mv | 2019-01-21T11:33:48Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Haemophilus influenzae COPD OM virulence factors biofilms NTHi |
| datacite.titles.title.fl_str_mv | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| dc.contributor.none.fl_str_mv | Lavado, Maria Paula Jordão, Maria Luísa RUN |
| dc.creator.none.fl_str_mv | Batista, Beatriz da Cunha |
| dc.date.Accepted.fl_str_mv | 2018-10-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-01-21T11:33:48Z |
| dc.date.embargoed.fl_str_mv | 2019-01-21T11:33:48Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/58090 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Haemophilus influenzae COPD OM virulence factors biofilms NTHi |
| dc.title.fl_str_mv | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | Haemophilus influenzae is a commensal microorganism of the human nasopharynx, responsible for both invasive and non-invasive diseases. This work focused on the study of 93 H. influenzae isolates, collected between 2013 and 2018, from patients with two epidemiologically relevant non-invasive diseases: Chronic Obstructive Pulmonary Disease (COPD) and Otitis Media (OM). Phenotypical and molecular characterizations were performed for the isolates, regarding capsular typing, β-lactamase production, antibiotic susceptibility, genetic diversity by MLST, presence/absence of virulence factors (pilA, hifA, hmw1A, hmw2A, hia and ompP5) and ability to produce biofilms. COPD isolates were collected from adults (100%), while 98.2% of OM isolates were collected from children. Non-typeable H. influenzae (NTHi) was the aetiological agent in COPD (97.4%) and OM (100%). Regarding antibiotic susceptibility, it should be noticed that 15.1% of the isolates were β- lactamase producers. MLST revealed a high genetic diversity among COPD and OM isolates, with 31 STs in 41 analysed isolates. pilA and ompP5 genes were present in more than 50% of COPD and OM isolates. hifA and hia genes were identified in less than half of the isolates, with a higher prevalence of these among OM isolates. hmw1A and hmw2A genes were respectively identified in 25.5% and 32.7% of OM isolates, while both hmw genes were present in 76.3% of COPD isolates. Biofilm production was observed for 14.0% and 29.0% of all isolates after 24h and 48h, respectively. No relationship between biofilm production and clinical source could be established, as well as with the presence of virulence factors (pilA, hmw1A e hmw2A) involved in biofilm production. COPD and OM are frequently associated with NTHi. Since no vaccines are available, monitoring of these diseases is highly recommended, as these constitute a Public Health threat associated with a high economic and social burden. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/93031bca-5e58-45ec-8868-b61c4c61ee56/download |
| id | run_4e1471ccfd5bc4e074bbf0a81a563dd1 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/58090 |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/58090 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Batista, Beatriz da Cunha |
| publishDate | 2018 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engpt_PTHaemophilus influenzae is a commensal microorganism of the human nasopharynx, responsible for both invasive and non-invasive diseases. This work focused on the study of 93 H. influenzae isolates, collected between 2013 and 2018, from patients with two epidemiologically relevant non-invasive diseases: Chronic Obstructive Pulmonary Disease (COPD) and Otitis Media (OM). Phenotypical and molecular characterizations were performed for the isolates, regarding capsular typing, β-lactamase production, antibiotic susceptibility, genetic diversity by MLST, presence/absence of virulence factors (pilA, hifA, hmw1A, hmw2A, hia and ompP5) and ability to produce biofilms. COPD isolates were collected from adults (100%), while 98.2% of OM isolates were collected from children. Non-typeable H. influenzae (NTHi) was the aetiological agent in COPD (97.4%) and OM (100%). Regarding antibiotic susceptibility, it should be noticed that 15.1% of the isolates were β- lactamase producers. MLST revealed a high genetic diversity among COPD and OM isolates, with 31 STs in 41 analysed isolates. pilA and ompP5 genes were present in more than 50% of COPD and OM isolates. hifA and hia genes were identified in less than half of the isolates, with a higher prevalence of these among OM isolates. hmw1A and hmw2A genes were respectively identified in 25.5% and 32.7% of OM isolates, while both hmw genes were present in 76.3% of COPD isolates. Biofilm production was observed for 14.0% and 29.0% of all isolates after 24h and 48h, respectively. No relationship between biofilm production and clinical source could be established, as well as with the presence of virulence factors (pilA, hmw1A e hmw2A) involved in biofilm production. COPD and OM are frequently associated with NTHi. Since no vaccines are available, monitoring of these diseases is highly recommended, as these constitute a Public Health threat associated with a high economic and social burden.application/pdfpt_PTCharacterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis MediaBatista, Beatriz da CunhaLavado, Maria PaulaJordão, Maria LuísaHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.pt2019-01-21T11:33:48Z2018-1020182018-10-01T00:00:00ZHandlehttp://hdl.handle.net/10362/58090http://purl.org/coar/access_right/c_abf2open accessHaemophilus influenzaeCOPDOMvirulence factorsbiofilmsNTHi4741063 bytesliteraturehttp://purl.org/coar/resource_type/c_bdccmaster thesishttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/93031bca-5e58-45ec-8868-b61c4c61ee56/download |
| spellingShingle | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media Batista, Beatriz da Cunha Haemophilus influenzae COPD OM virulence factors biofilms NTHi Batista, Beatriz da Cunha Haemophilus influenzae COPD OM virulence factors biofilms NTHi |
| status | NEW |
| subject.fl_str_mv | Haemophilus influenzae COPD OM virulence factors biofilms NTHi |
| title | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| title_full | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| title_fullStr | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| title_full_unstemmed | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| title_short | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| title_sort | Characterization and Biofilm Assessment of Haemophilus influenzae Isolates from Patients with Chronic Obstructive Pulmonary Disease and Otitis Media |
| topic | Haemophilus influenzae COPD OM virulence factors biofilms NTHi |
| topic_facet | Haemophilus influenzae COPD OM virulence factors biofilms NTHi |
| url | http://hdl.handle.net/10362/58090 |
| visible | 1 |