Publicação
Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue
| Resumo: | Dopamine directly acts in the liver and white adipose tissue (WAT) to regulate insulin signaling, glucose uptake, and catabolic activity. Given that dopamine is secreted by the gut and regulates insulin secretion in the pancreas, we aimed to determine its regulation by nutritional cues and its role in regulating glucagon-like peptide 1 (GLP-1) action in WAT. Solutions with different nutrients were administered to Wistar rats and postprandial dopamine levels showed elevations following a mixed meal and glucose intake. In high-fat diet-fed diabetic Goto-Kakizaki rats, sleeve gastrectomy upregulated dopaminergic machinery, showing the role of the gut in dopamine signaling in WAT. Bromocriptine treatment in the same model increased GLP-1R in WAT, showing the role of dopamine in regulating GLP-1R. By contrast, treatment with the GLP-1 receptor agonist Liraglutide had no impact on dopamine receptors. GLP-1 and dopamine crosstalk was shown in rat WAT explants, since dopamine upregulated GLP-1-induced AMPK activity in mesenteric WAT in the presence of the D2R and D3R inhibitor Domperidone. In human WAT, dopamine receptor 1 (D1DR) and GLP-1R expression were correlated. Our results point out a dietary and gut regulation of plasma dopamine, acting in the WAT to regulate GLP-1 action. Together with the known dopamine action in the pancreas, such results may identify new therapeutic opportunities to improve metabolic control in metabolic disorders. |
|---|---|
| Autores principais: | Tavares, Gabriela |
| Outros Autores: | Rosendo-Silva, Daniela; Simões, Flávia; Eickhoff, Hans; Marques, Daniela; Sacramento, Joana F.; Capucho, Adriana M.; Seiça, Raquel; Conde, Sílvia V.; V Conde, Silvia; Matafome, Paulo |
| Assunto: | dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1865920613236867072 |
|---|---|
| author | Tavares, Gabriela |
| author2 | Rosendo-Silva, Daniela Simões, Flávia Eickhoff, Hans Marques, Daniela Sacramento, Joana F. Capucho, Adriana M. Seiça, Raquel Conde, Sílvia V. V Conde, Silvia Matafome, Paulo |
| author2_role | author author author author author author author author author author |
| author_facet | Tavares, Gabriela Tavares, Gabriela Rosendo-Silva, Daniela Simões, Flávia Eickhoff, Hans Marques, Daniela Sacramento, Joana F. Capucho, Adriana M. Seiça, Raquel Conde, Sílvia V. V Conde, Silvia Matafome, Paulo Rosendo-Silva, Daniela Simões, Flávia Eickhoff, Hans Marques, Daniela Sacramento, Joana F. Capucho, Adriana M. Seiça, Raquel Conde, Sílvia V. V Conde, Silvia Matafome, Paulo |
| author_role | author |
| contributor_name_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Tavares, Gabriela\"},{\"Person.name\":\"Rosendo-Silva, Daniela\"},{\"Person.name\":\"Simões, Flávia\"},{\"Person.name\":\"Eickhoff, Hans\"},{\"Person.name\":\"Marques, Daniela\"},{\"Person.name\":\"Sacramento, Joana F.\"},{\"Person.name\":\"Capucho, Adriana M.\"},{\"Person.name\":\"Seiça, Raquel\"},{\"Person.name\":\"Conde, Sílvia V.\"},{\"Person.name\":\"V Conde, Silvia\",\"Person.identifier.orcid\":\"0000-0002-5920-5700\"},{\"Person.name\":\"Matafome, Paulo\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Tavares, Gabriela Rosendo-Silva, Daniela Simões, Flávia Eickhoff, Hans Marques, Daniela Sacramento, Joana F. Capucho, Adriana M. Seiça, Raquel Conde, Sílvia V. V Conde, Silvia Matafome, Paulo |
| datacite.date.Accepted.fl_str_mv | 2023-02-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-02-23T22:21:15Z |
| datacite.date.embargoed.fl_str_mv | 2023-02-23T22:21:15Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| dc.contributor.none.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| dc.creator.none.fl_str_mv | Tavares, Gabriela Rosendo-Silva, Daniela Simões, Flávia Eickhoff, Hans Marques, Daniela Sacramento, Joana F. Capucho, Adriana M. Seiça, Raquel Conde, Sílvia V. V Conde, Silvia Matafome, Paulo |
| dc.date.Accepted.fl_str_mv | 2023-02-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-02-23T22:21:15Z |
| dc.date.embargoed.fl_str_mv | 2023-02-23T22:21:15Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/149626 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Dopamine directly acts in the liver and white adipose tissue (WAT) to regulate insulin signaling, glucose uptake, and catabolic activity. Given that dopamine is secreted by the gut and regulates insulin secretion in the pancreas, we aimed to determine its regulation by nutritional cues and its role in regulating glucagon-like peptide 1 (GLP-1) action in WAT. Solutions with different nutrients were administered to Wistar rats and postprandial dopamine levels showed elevations following a mixed meal and glucose intake. In high-fat diet-fed diabetic Goto-Kakizaki rats, sleeve gastrectomy upregulated dopaminergic machinery, showing the role of the gut in dopamine signaling in WAT. Bromocriptine treatment in the same model increased GLP-1R in WAT, showing the role of dopamine in regulating GLP-1R. By contrast, treatment with the GLP-1 receptor agonist Liraglutide had no impact on dopamine receptors. GLP-1 and dopamine crosstalk was shown in rat WAT explants, since dopamine upregulated GLP-1-induced AMPK activity in mesenteric WAT in the presence of the D2R and D3R inhibitor Domperidone. In human WAT, dopamine receptor 1 (D1DR) and GLP-1R expression were correlated. Our results point out a dietary and gut regulation of plasma dopamine, acting in the WAT to regulate GLP-1 action. Together with the known dopamine action in the pancreas, such results may identify new therapeutic opportunities to improve metabolic control in metabolic disorders. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/5289ad3b-db5e-432a-b399-cfc85062ed4d/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10362/149626 |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/149626 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Tavares, Gabriela Rosendo-Silva, Daniela Simões, Flávia Eickhoff, Hans Marques, Daniela Sacramento, Joana F. Capucho, Adriana M. Seiça, Raquel Conde, Sílvia V. V Conde, Silvia V Conde, Silvia https://www.ciencia-id.pt/561E-CF29-600A 561E-CF29-600A http://orcid.org/0000-0002-5920-5700 0000-0002-5920-5700 Matafome, Paulo |
| publishDate | 2023 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenDopamine directly acts in the liver and white adipose tissue (WAT) to regulate insulin signaling, glucose uptake, and catabolic activity. Given that dopamine is secreted by the gut and regulates insulin secretion in the pancreas, we aimed to determine its regulation by nutritional cues and its role in regulating glucagon-like peptide 1 (GLP-1) action in WAT. Solutions with different nutrients were administered to Wistar rats and postprandial dopamine levels showed elevations following a mixed meal and glucose intake. In high-fat diet-fed diabetic Goto-Kakizaki rats, sleeve gastrectomy upregulated dopaminergic machinery, showing the role of the gut in dopamine signaling in WAT. Bromocriptine treatment in the same model increased GLP-1R in WAT, showing the role of dopamine in regulating GLP-1R. By contrast, treatment with the GLP-1 receptor agonist Liraglutide had no impact on dopamine receptors. GLP-1 and dopamine crosstalk was shown in rat WAT explants, since dopamine upregulated GLP-1-induced AMPK activity in mesenteric WAT in the presence of the D2R and D3R inhibitor Domperidone. In human WAT, dopamine receptor 1 (D1DR) and GLP-1R expression were correlated. Our results point out a dietary and gut regulation of plasma dopamine, acting in the WAT to regulate GLP-1 action. Together with the known dopamine action in the pancreas, such results may identify new therapeutic opportunities to improve metabolic control in metabolic disorders.application/pdfenCirculating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose TissueTavares, GabrielaRosendo-Silva, DanielaSimões, FláviaEickhoff, HansMarques, DanielaSacramento, Joana F.Capucho, Adriana M.Seiça, RaquelConde, Sílvia V.PersonalV Conde, SilviaDSpacehttp://dspace.org/items/898e99d8-61a5-44bc-878a-5e1ac44ca37cDSpacehttp://dspace.org/items/898e99d8-61a5-44bc-878a-5e1ac44ca37cVilares CondeSilviaCiência IDhttps://www.ciencia-id.pt561E-CF29-600AORCIDhttp://orcid.org0000-0002-5920-5700Scopus Author IDhttps://www.scopus.com7004283202Scopus Author IDhttps://www.scopus.com7004283202Matafome, PauloNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)MDPI - Multidisciplinary Digital Publishing InstituteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1661-6596URNIsPartOfPURE: 53993553URNIsPartOfPURE UUID: 2f5139ab-9d4d-4e03-a5ba-faa508edaaf5URNIsPartOfScopus: 85147893270URNIsPartOfPubMed: 36768789URNIsPartOfORCID: /0000-0002-5920-5700/work/129548070URNIsPartOfWOS: 000932559900001DOIIsPartOf10.3390/ijms240324642023-02-23T22:21:15Z2023-022023-02-01T00:00:00ZHandlehttp://hdl.handle.net/10362/149626http://purl.org/coar/access_right/c_abf2open accessdopamineGLP-1white adipose tissueCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistrySDG 3 - Good Health and Well-being4208200 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/5289ad3b-db5e-432a-b399-cfc85062ed4d/download |
| spellingShingle | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue Tavares, Gabriela dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being Tavares, Gabriela dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| status | NEW |
| subject.fl_str_mv | dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| title | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| title_full | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| title_fullStr | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| title_full_unstemmed | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| title_short | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| title_sort | Circulating Dopamine Is Regulated by Dietary Glucose and Controls Glucagon-like 1 Peptide Action in White Adipose Tissue |
| topic | dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| topic_facet | dopamine GLP-1 white adipose tissue Catalysis Molecular Biology Spectroscopy Computer Science Applications Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/149626 |
| visible | 1 |