Publication
Exploring respiratory enzymes from Staphylococcus aureus
| Summary: | "Staphylococcus aureus is an opportunistic pathogen that can cause various disease patterns due to its high adaptative capacity. Many aspects of the energy metabolism and its respiratory chain system are still poorly understood. Herein, we report our study of three respiratory enzymes: Membrane potential- generating system (MpsAB) and two malate:quinone oxidoreductases (MQO). The MpsAB enzyme is a membrane complex proposed to be involved in energy conservation in S. aureus due the sequence homology of the subunit MpsA with the proton-translocation subunit NuoL of complex I from Escherichia coli. To further investigate the complex a series of expression tests were made to achieve its heterologous expression in E. coli. We also tried to optimize protein purification for biochemical studies. (...)" |
|---|---|
| Main Authors: | Barbosa, Catarina |
| Subject: | Energy Metabolism Respiratory Chain Membrane Potential-Generating System AB Malate:quinone Oxidoreductases |
| Year: | 2018 |
| Country: | Portugal |
| Document type: | master thesis |
| Access type: | open access |
| Associated institution: | Universidade Nova de Lisboa |
| Language: | English |
| Origin: | Repositório Institucional da UNL |
| _version_ | 1868414815284756480 |
|---|---|
| author | Barbosa, Catarina |
| author_facet | Barbosa, Catarina |
| author_role | author |
| contributor_name_str_mv | Pereira, Manuela Refojo, Ana RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Barbosa, Catarina\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Pereira, Manuela Refojo, Ana RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Barbosa, Catarina |
| datacite.date.Accepted.fl_str_mv | 2018-12-06T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-01-01T22:41:42Z |
| datacite.date.embargoed.fl_str_mv | 2022-01-01T22:41:42Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Energy Metabolism Respiratory Chain Membrane Potential-Generating System AB Malate:quinone Oxidoreductases |
| datacite.titles.title.fl_str_mv | Exploring respiratory enzymes from Staphylococcus aureus |
| dc.contributor.none.fl_str_mv | Pereira, Manuela Refojo, Ana RUN |
| dc.creator.none.fl_str_mv | Barbosa, Catarina |
| dc.date.Accepted.fl_str_mv | 2018-12-06T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-01-01T22:41:42Z |
| dc.date.embargoed.fl_str_mv | 2022-01-01T22:41:42Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/130064 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Energy Metabolism Respiratory Chain Membrane Potential-Generating System AB Malate:quinone Oxidoreductases |
| dc.title.fl_str_mv | Exploring respiratory enzymes from Staphylococcus aureus |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | "Staphylococcus aureus is an opportunistic pathogen that can cause various disease patterns due to its high adaptative capacity. Many aspects of the energy metabolism and its respiratory chain system are still poorly understood. Herein, we report our study of three respiratory enzymes: Membrane potential- generating system (MpsAB) and two malate:quinone oxidoreductases (MQO). The MpsAB enzyme is a membrane complex proposed to be involved in energy conservation in S. aureus due the sequence homology of the subunit MpsA with the proton-translocation subunit NuoL of complex I from Escherichia coli. To further investigate the complex a series of expression tests were made to achieve its heterologous expression in E. coli. We also tried to optimize protein purification for biochemical studies. (...)" |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/d9fca6ac-4034-4c4d-94d2-7f82e7f91424/download |
| id | run_6b5903ed5c19de1feb98d92f8e8aa435 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/130064 |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/130064 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Barbosa, Catarina Barbosa, Catarina https://www.ciencia-id.pt/DA15-5D7E-D995 DA15-5D7E-D995 |
| publishDate | 2018 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engpt_PT"Staphylococcus aureus is an opportunistic pathogen that can cause various disease patterns due to its high adaptative capacity. Many aspects of the energy metabolism and its respiratory chain system are still poorly understood. Herein, we report our study of three respiratory enzymes: Membrane potential- generating system (MpsAB) and two malate:quinone oxidoreductases (MQO). The MpsAB enzyme is a membrane complex proposed to be involved in energy conservation in S. aureus due the sequence homology of the subunit MpsA with the proton-translocation subunit NuoL of complex I from Escherichia coli. To further investigate the complex a series of expression tests were made to achieve its heterologous expression in E. coli. We also tried to optimize protein purification for biochemical studies. (...)"application/pdfpt_PTExploring respiratory enzymes from Staphylococcus aureusPersonalBarbosa, CatarinaDSpacehttp://dspace.org/items/95334600-b7db-4362-8176-5a1c831773efDSpacehttp://dspace.org/items/95334600-b7db-4362-8176-5a1c831773efBoloto BarbosaCatarinaCiência IDhttps://www.ciencia-id.ptDA15-5D7E-D995Pereira, ManuelaRefojo, AnaHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.pt2022-01-01T22:41:42Z2018-12-062018-102018-12-06T00:00:00ZHandlehttp://hdl.handle.net/10362/130064http://purl.org/coar/access_right/c_abf2open accessEnergy MetabolismRespiratory ChainMembrane Potential-Generating System ABMalate:quinone Oxidoreductases4902141 bytesliteraturehttp://purl.org/coar/resource_type/c_bdccmaster thesis2018-12-06http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/d9fca6ac-4034-4c4d-94d2-7f82e7f91424/download |
| spellingShingle | Exploring respiratory enzymes from Staphylococcus aureus Barbosa, Catarina Energy Metabolism Respiratory Chain Membrane Potential-Generating System AB Malate:quinone Oxidoreductases |
| status | SINGLETON |
| subject.fl_str_mv | Energy Metabolism Respiratory Chain Membrane Potential-Generating System AB Malate:quinone Oxidoreductases |
| title | Exploring respiratory enzymes from Staphylococcus aureus |
| title_full | Exploring respiratory enzymes from Staphylococcus aureus |
| title_fullStr | Exploring respiratory enzymes from Staphylococcus aureus |
| title_full_unstemmed | Exploring respiratory enzymes from Staphylococcus aureus |
| title_short | Exploring respiratory enzymes from Staphylococcus aureus |
| title_sort | Exploring respiratory enzymes from Staphylococcus aureus |
| topic | Energy Metabolism Respiratory Chain Membrane Potential-Generating System AB Malate:quinone Oxidoreductases |
| topic_facet | Energy Metabolism Respiratory Chain Membrane Potential-Generating System AB Malate:quinone Oxidoreductases |
| url | http://hdl.handle.net/10362/130064 |
| visible | 1 |