Publicação
Adaptive potential of hybridization among malaria vectors
| Resumo: | "Far-West" Africa is known to be a secondary contact zone between the two major malaria vectors Anopheles coluzzii and A. gambiae. We investigated gene-flow and potentially adaptive introgression between these species along a west-to-east transect in Guinea Bissau, the putative core of this hybrid zone. To evaluate the extent and direction of gene flow, we genotyped site 702 in Intron-1 of the para Voltage-Gated Sodium Channel gene, a species-diagnostic nucleotide position throughout most of A. coluzzii and A. gambiae sympatric range. We also analyzed polymorphism in the thioester-binding domain (TED) of the innate immunity-linked thioester-containing protein 1 (TEP1) to investigate whether elevated hybridization might facilitate the exchange of variants linked to adaptive immunity and Plasmodium refractoriness. Our results confirm asymmetric introgression of genetic material from A. coluzzii to A. gambiae and disruption of linkage between the centromeric "genomic islands" of inter-specific divergence. We report that A. gambiae from the Guinean hybrid zone possesses an introgressed TEP1 resistant allelic class, found exclusively in A. coluzzii elsewhere and apparently swept to fixation in West Africa (i.e. Mali and Burkina Faso). However, no detectable fixation of this allele was found in Guinea Bissau, which may suggest that ecological pressures driving segregation between the two species in larval habitats in this region may be different from those experienced in northern and more arid parts of the species' range. Finally, our results also suggest a genetic subdivision between coastal and inland A. gambiae Guinean populations and provide clues on the importance of ecological factors in intra-specific differentiation processes. |
|---|---|
| Autores principais: | Mancini, Emiliano |
| Outros Autores: | Spinaci, Maria Ida; Gordicho, Vasco; Caputo, Beniamino; Pombi, Marco; Vicente, José Luis; Dinis, João; Rodrigues, Amabélia; Petrarca, Vincenzo; Weetman, David; Pinto, João; Torre, Alessandra Della |
| Assunto: | Ecology, Evolution, Behavior and Systematics Genetics Insect Science Infectious Diseases Immunology SDG 3 - Good Health and Well-being |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983702883663872 |
|---|---|
| author | Mancini, Emiliano |
| author2 | Spinaci, Maria Ida Gordicho, Vasco Caputo, Beniamino Pombi, Marco Vicente, José Luis Dinis, João Rodrigues, Amabélia Petrarca, Vincenzo Weetman, David Pinto, João Torre, Alessandra Della |
| author2_role | author author author author author author author author author author author |
| author_facet | Mancini, Emiliano Spinaci, Maria Ida Gordicho, Vasco Caputo, Beniamino Pombi, Marco Vicente, José Luis Dinis, João Rodrigues, Amabélia Petrarca, Vincenzo Weetman, David Pinto, João Torre, Alessandra Della |
| author_role | author |
| contributor_name_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) PLOS - Public Library of Science RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Mancini, Emiliano\"},{\"Person.name\":\"Spinaci, Maria Ida\"},{\"Person.name\":\"Gordicho, Vasco\"},{\"Person.name\":\"Caputo, Beniamino\"},{\"Person.name\":\"Pombi, Marco\"},{\"Person.name\":\"Vicente, José Luis\"},{\"Person.name\":\"Dinis, João\"},{\"Person.name\":\"Rodrigues, Amabélia\"},{\"Person.name\":\"Petrarca, Vincenzo\"},{\"Person.name\":\"Weetman, David\"},{\"Person.name\":\"Pinto, João\"},{\"Person.name\":\"Torre, Alessandra Della\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) PLOS - Public Library of Science RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Mancini, Emiliano Spinaci, Maria Ida Gordicho, Vasco Caputo, Beniamino Pombi, Marco Vicente, José Luis Dinis, João Rodrigues, Amabélia Petrarca, Vincenzo Weetman, David Pinto, João Torre, Alessandra Della |
| datacite.date.Accepted.fl_str_mv | 2015-06-05T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-05-14T22:02:06Z |
| datacite.date.embargoed.fl_str_mv | 2018-05-14T22:02:06Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ecology, Evolution, Behavior and Systematics Genetics Insect Science Infectious Diseases Immunology SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Adaptive potential of hybridization among malaria vectors Introgression at the immune locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa |
| dc.contributor.none.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) PLOS - Public Library of Science RUN |
| dc.creator.none.fl_str_mv | Mancini, Emiliano Spinaci, Maria Ida Gordicho, Vasco Caputo, Beniamino Pombi, Marco Vicente, José Luis Dinis, João Rodrigues, Amabélia Petrarca, Vincenzo Weetman, David Pinto, João Torre, Alessandra Della |
| dc.date.Accepted.fl_str_mv | 2015-06-05T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-05-14T22:02:06Z |
| dc.date.embargoed.fl_str_mv | 2018-05-14T22:02:06Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://www.scopus.com/inward/record.url?scp=84934942312&partnerID=8YFLogxK |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Ecology, Evolution, Behavior and Systematics Genetics Insect Science Infectious Diseases Immunology SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Adaptive potential of hybridization among malaria vectors Introgression at the immune locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | "Far-West" Africa is known to be a secondary contact zone between the two major malaria vectors Anopheles coluzzii and A. gambiae. We investigated gene-flow and potentially adaptive introgression between these species along a west-to-east transect in Guinea Bissau, the putative core of this hybrid zone. To evaluate the extent and direction of gene flow, we genotyped site 702 in Intron-1 of the para Voltage-Gated Sodium Channel gene, a species-diagnostic nucleotide position throughout most of A. coluzzii and A. gambiae sympatric range. We also analyzed polymorphism in the thioester-binding domain (TED) of the innate immunity-linked thioester-containing protein 1 (TEP1) to investigate whether elevated hybridization might facilitate the exchange of variants linked to adaptive immunity and Plasmodium refractoriness. Our results confirm asymmetric introgression of genetic material from A. coluzzii to A. gambiae and disruption of linkage between the centromeric "genomic islands" of inter-specific divergence. We report that A. gambiae from the Guinean hybrid zone possesses an introgressed TEP1 resistant allelic class, found exclusively in A. coluzzii elsewhere and apparently swept to fixation in West Africa (i.e. Mali and Burkina Faso). However, no detectable fixation of this allele was found in Guinea Bissau, which may suggest that ecological pressures driving segregation between the two species in larval habitats in this region may be different from those experienced in northern and more arid parts of the species' range. Finally, our results also suggest a genetic subdivision between coastal and inland A. gambiae Guinean populations and provide clues on the importance of ecological factors in intra-specific differentiation processes. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/64dfe451-2f17-4d02-bf6f-46e714a6f40a/download |
| id | run_77d3fadc8b2c17fbb9fcc5a3a4b93fc6 |
| identifier.url.fl_str_mv | http://www.scopus.com/inward/record.url?scp=84934942312&partnerID=8YFLogxK |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/36939 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Mancini, Emiliano Spinaci, Maria Ida Gordicho, Vasco Caputo, Beniamino Pombi, Marco Vicente, José Luis Dinis, João Rodrigues, Amabélia Petrarca, Vincenzo Weetman, David Pinto, João Torre, Alessandra Della |
| publishDate | 2015 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engen"Far-West" Africa is known to be a secondary contact zone between the two major malaria vectors Anopheles coluzzii and A. gambiae. We investigated gene-flow and potentially adaptive introgression between these species along a west-to-east transect in Guinea Bissau, the putative core of this hybrid zone. To evaluate the extent and direction of gene flow, we genotyped site 702 in Intron-1 of the para Voltage-Gated Sodium Channel gene, a species-diagnostic nucleotide position throughout most of A. coluzzii and A. gambiae sympatric range. We also analyzed polymorphism in the thioester-binding domain (TED) of the innate immunity-linked thioester-containing protein 1 (TEP1) to investigate whether elevated hybridization might facilitate the exchange of variants linked to adaptive immunity and Plasmodium refractoriness. Our results confirm asymmetric introgression of genetic material from A. coluzzii to A. gambiae and disruption of linkage between the centromeric "genomic islands" of inter-specific divergence. We report that A. gambiae from the Guinean hybrid zone possesses an introgressed TEP1 resistant allelic class, found exclusively in A. coluzzii elsewhere and apparently swept to fixation in West Africa (i.e. Mali and Burkina Faso). However, no detectable fixation of this allele was found in Guinea Bissau, which may suggest that ecological pressures driving segregation between the two species in larval habitats in this region may be different from those experienced in northern and more arid parts of the species' range. Finally, our results also suggest a genetic subdivision between coastal and inland A. gambiae Guinean populations and provide clues on the importance of ecological factors in intra-specific differentiation processes.application/pdfenAdaptive potential of hybridization among malaria vectorsSubtitleenIntrogression at the immune locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' AfricaMancini, EmilianoSpinaci, Maria IdaGordicho, VascoCaputo, BeniaminoPombi, MarcoVicente, José LuisDinis, JoãoRodrigues, AmabéliaPetrarca, VincenzoWeetman, DavidPinto, JoãoTorre, Alessandra DellaInstituto de Higiene e Medicina Tropical (IHMT)Centro de Malária e outras Doenças Tropicais (CMDT)PLOS - Public Library of ScienceHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1932-6203URNIsPartOfPURE: 1728870URNIsPartOfPURE UUID: bf07dd9f-c718-467c-b108-d03f78e1514eURNIsPartOfScopus: 84934942312URNIsPartOfWOS: 000355652200031URNIsPartOfPubMed: 26047479URNIsPartOfORCID: /0000-0001-8572-7708/work/81722265DOIIsPartOf10.1371/journal.pone.01278042018-05-14T22:02:06Z2015-06-052015-06-05T00:00:00ZURLhttp://www.scopus.com/inward/record.url?scp=84934942312&partnerID=8YFLogxKhttp://purl.org/coar/access_right/c_abf2open accessEcology, Evolution, Behavior and SystematicsGeneticsInsect ScienceInfectious DiseasesImmunologySDG 3 - Good Health and Well-being1383379 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/64dfe451-2f17-4d02-bf6f-46e714a6f40a/download |
| spellingShingle | Adaptive potential of hybridization among malaria vectors Mancini, Emiliano Ecology, Evolution, Behavior and Systematics Genetics Insect Science Infectious Diseases Immunology SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | Ecology, Evolution, Behavior and Systematics Genetics Insect Science Infectious Diseases Immunology SDG 3 - Good Health and Well-being |
| title | Adaptive potential of hybridization among malaria vectors |
| title_full | Adaptive potential of hybridization among malaria vectors |
| title_fullStr | Adaptive potential of hybridization among malaria vectors |
| title_full_unstemmed | Adaptive potential of hybridization among malaria vectors |
| title_short | Adaptive potential of hybridization among malaria vectors |
| title_sort | Adaptive potential of hybridization among malaria vectors |
| topic | Ecology, Evolution, Behavior and Systematics Genetics Insect Science Infectious Diseases Immunology SDG 3 - Good Health and Well-being |
| topic_facet | Ecology, Evolution, Behavior and Systematics Genetics Insect Science Infectious Diseases Immunology SDG 3 - Good Health and Well-being |
| url | http://www.scopus.com/inward/record.url?scp=84934942312&partnerID=8YFLogxK |
| visible | 1 |