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Toxicogenic fungi and mycotoxins seasonality in poultry farms

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Resumo:Poultry farms are hostpost for fungi and mycotoxins proliferation. Still, seasonal dynamics on of these hazards on farm and their impacts on animals/workers health, and lastly on food safety remain unexplored. This study provides the first comprehensive assessment of seasonal (summer/winter) fungal/mycotoxins exposure in poultry farms environment (Electrostatic dust cloths (EDC), feed, bed) and broiler chicken tissues (breast, liver) to identify potential exposure risks for broiler chickens/workers health and food safety. Viable mycotoxigenic fungi and mycotoxins profile (LC-MS/MS) was characterized and cytotoxicity assessment was performed using A549 (human alveolar epithelial) and SK (swine kidney) cells. Zootechnical parameters were also measured to evaluate broiler chickens’ growth performance. Summer conditions favour the proliferation of mycotoxigenic fungi belonging to Penicillium and Aspergillus genera, where co-contamination of hazardous mycotoxins (ZEN/TRCs/FBs) was prevalent. Considering cytotoxicity assessment, EDC samples induce high toxicity in 47 % of A549 cells during summer, whereas on bed samples, high toxicity was obtained during winter on both cell lines (100 % SK cells; 83 % A549 cells). Our results evidence diverse exposure pathways (inhalation, dermal contact) and seasonal health risks. Regarding mycotoxins in biological samples, ZEN was detected in 53.3 % of liver samples, highlighting chronic exposure risks. Thus, future research should focus on toxicokinetics/toxicodynamics of co-occurring mycotoxins in animal production environments and their subsequent human exposure through the food chain. Given that climate change may exacerbate seasonal fungal/mycotoxin contamination, understanding these interactions is crucial for improving risk assessment frameworks and to implement protection measures on the farm level and along the food chain.
Autores principais:Gomes, Bianca
Outros Autores:Dias, Marta; Cervantes, Renata; Pena, Pedro; Twarużek, Magdalena; Kosicki, Robert; Grajewski, Jan; Carolino, Elisabete; Viegas, Susana; Viegas, Carla
Assunto:Food safety Growth performance Mycotoxins Poultry production Toxigenic fungi Food Science Agronomy and Crop Science Agricultural and Biological Sciences (miscellaneous) SDG 3 - Good Health and Well-being SDG 13 - Climate Action
Ano:2025
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade Nova de Lisboa
Idioma:inglês
Origem:Repositório Institucional da UNL
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author Gomes, Bianca
author2 Dias, Marta
Cervantes, Renata
Pena, Pedro
Twarużek, Magdalena
Kosicki, Robert
Grajewski, Jan
Carolino, Elisabete
Viegas, Susana
Viegas, Carla
author2_role author
author
author
author
author
author
author
author
author
author_facet Gomes, Bianca
Dias, Marta
Cervantes, Renata
Pena, Pedro
Twarużek, Magdalena
Kosicki, Robert
Grajewski, Jan
Carolino, Elisabete
Viegas, Susana
Viegas, Carla
author_role author
contributor_name_str_mv Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP)
Centro de Investigação em Saúde Pública (CISP/PHRC)
Comprehensive Health Research Centre (CHRC) - Pólo ENSP
Escola Nacional de Saúde Pública (ENSP)
Elsevier BV
RUN
country_str PT
creators_json_txt [{\"Person.name\":\"Gomes, Bianca\"},{\"Person.name\":\"Dias, Marta\"},{\"Person.name\":\"Cervantes, Renata\"},{\"Person.name\":\"Pena, Pedro\"},{\"Person.name\":\"Twarużek, Magdalena\"},{\"Person.name\":\"Kosicki, Robert\"},{\"Person.name\":\"Grajewski, Jan\"},{\"Person.name\":\"Carolino, Elisabete\"},{\"Person.name\":\"Viegas, Susana\"},{\"Person.name\":\"Viegas, Carla\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP)
Centro de Investigação em Saúde Pública (CISP/PHRC)
Comprehensive Health Research Centre (CHRC) - Pólo ENSP
Escola Nacional de Saúde Pública (ENSP)
Elsevier BV
RUN
datacite.creators.creator.creatorName.fl_str_mv Gomes, Bianca
Dias, Marta
Cervantes, Renata
Pena, Pedro
Twarużek, Magdalena
Kosicki, Robert
Grajewski, Jan
Carolino, Elisabete
Viegas, Susana
Viegas, Carla
datacite.date.Accepted.fl_str_mv 2025-12-01T00:00:00Z
datacite.date.available.fl_str_mv 2025-10-23T21:58:12Z
datacite.date.embargoed.fl_str_mv 2025-10-23T21:58:12Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Food safety
Growth performance
Mycotoxins
Poultry production
Toxigenic fungi
Food Science
Agronomy and Crop Science
Agricultural and Biological Sciences (miscellaneous)
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
datacite.titles.title.fl_str_mv Toxicogenic fungi and mycotoxins seasonality in poultry farms
implications for animal health and food safety
dc.contributor.none.fl_str_mv Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP)
Centro de Investigação em Saúde Pública (CISP/PHRC)
Comprehensive Health Research Centre (CHRC) - Pólo ENSP
Escola Nacional de Saúde Pública (ENSP)
Elsevier BV
RUN
dc.creator.none.fl_str_mv Gomes, Bianca
Dias, Marta
Cervantes, Renata
Pena, Pedro
Twarużek, Magdalena
Kosicki, Robert
Grajewski, Jan
Carolino, Elisabete
Viegas, Susana
Viegas, Carla
dc.date.Accepted.fl_str_mv 2025-12-01T00:00:00Z
dc.date.available.fl_str_mv 2025-10-23T21:58:12Z
dc.date.embargoed.fl_str_mv 2025-10-23T21:58:12Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10362/189670
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Food safety
Growth performance
Mycotoxins
Poultry production
Toxigenic fungi
Food Science
Agronomy and Crop Science
Agricultural and Biological Sciences (miscellaneous)
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
dc.title.fl_str_mv Toxicogenic fungi and mycotoxins seasonality in poultry farms
implications for animal health and food safety
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Poultry farms are hostpost for fungi and mycotoxins proliferation. Still, seasonal dynamics on of these hazards on farm and their impacts on animals/workers health, and lastly on food safety remain unexplored. This study provides the first comprehensive assessment of seasonal (summer/winter) fungal/mycotoxins exposure in poultry farms environment (Electrostatic dust cloths (EDC), feed, bed) and broiler chicken tissues (breast, liver) to identify potential exposure risks for broiler chickens/workers health and food safety. Viable mycotoxigenic fungi and mycotoxins profile (LC-MS/MS) was characterized and cytotoxicity assessment was performed using A549 (human alveolar epithelial) and SK (swine kidney) cells. Zootechnical parameters were also measured to evaluate broiler chickens’ growth performance. Summer conditions favour the proliferation of mycotoxigenic fungi belonging to Penicillium and Aspergillus genera, where co-contamination of hazardous mycotoxins (ZEN/TRCs/FBs) was prevalent. Considering cytotoxicity assessment, EDC samples induce high toxicity in 47 % of A549 cells during summer, whereas on bed samples, high toxicity was obtained during winter on both cell lines (100 % SK cells; 83 % A549 cells). Our results evidence diverse exposure pathways (inhalation, dermal contact) and seasonal health risks. Regarding mycotoxins in biological samples, ZEN was detected in 53.3 % of liver samples, highlighting chronic exposure risks. Thus, future research should focus on toxicokinetics/toxicodynamics of co-occurring mycotoxins in animal production environments and their subsequent human exposure through the food chain. Given that climate change may exacerbate seasonal fungal/mycotoxin contamination, understanding these interactions is crucial for improving risk assessment frameworks and to implement protection measures on the farm level and along the food chain.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://run.unl.pt/bitstreams/affcf702-ed61-4a13-a138-b441345ee68d/download
id run_8e51e93db3f95ee87f202fc3e21bd3fc
identifier.url.fl_str_mv http://hdl.handle.net/10362/189670
inst_facet_str urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa
instacron_str unl
institution Universidade Nova de Lisboa
instname_str Universidade Nova de Lisboa
language eng
network_acronym_str run
network_name_str Repositório Institucional da UNL
oai_identifier_str oai:run.unl.pt:10362/189670
organization_str_mv urn:organizationAcronym:unl
person_str_mv Gomes, Bianca
Dias, Marta
Cervantes, Renata
Pena, Pedro
Twarużek, Magdalena
Kosicki, Robert
Grajewski, Jan
Carolino, Elisabete
Viegas, Susana
Viegas, Carla
publishDate 2025
repo_facet_str urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL
reponame_str Repositório Institucional da UNL
repository_id_str urn:repositoryAcronym:run
service_str_mv urn:repositoryAcronym:run
spelling engenPoultry farms are hostpost for fungi and mycotoxins proliferation. Still, seasonal dynamics on of these hazards on farm and their impacts on animals/workers health, and lastly on food safety remain unexplored. This study provides the first comprehensive assessment of seasonal (summer/winter) fungal/mycotoxins exposure in poultry farms environment (Electrostatic dust cloths (EDC), feed, bed) and broiler chicken tissues (breast, liver) to identify potential exposure risks for broiler chickens/workers health and food safety. Viable mycotoxigenic fungi and mycotoxins profile (LC-MS/MS) was characterized and cytotoxicity assessment was performed using A549 (human alveolar epithelial) and SK (swine kidney) cells. Zootechnical parameters were also measured to evaluate broiler chickens’ growth performance. Summer conditions favour the proliferation of mycotoxigenic fungi belonging to Penicillium and Aspergillus genera, where co-contamination of hazardous mycotoxins (ZEN/TRCs/FBs) was prevalent. Considering cytotoxicity assessment, EDC samples induce high toxicity in 47 % of A549 cells during summer, whereas on bed samples, high toxicity was obtained during winter on both cell lines (100 % SK cells; 83 % A549 cells). Our results evidence diverse exposure pathways (inhalation, dermal contact) and seasonal health risks. Regarding mycotoxins in biological samples, ZEN was detected in 53.3 % of liver samples, highlighting chronic exposure risks. Thus, future research should focus on toxicokinetics/toxicodynamics of co-occurring mycotoxins in animal production environments and their subsequent human exposure through the food chain. Given that climate change may exacerbate seasonal fungal/mycotoxin contamination, understanding these interactions is crucial for improving risk assessment frameworks and to implement protection measures on the farm level and along the food chain.application/pdfenToxicogenic fungi and mycotoxins seasonality in poultry farmsSubtitleenimplications for animal health and food safetyGomes, BiancaDias, MartaCervantes, RenataPena, PedroTwarużek, MagdalenaKosicki, RobertGrajewski, JanCarolino, ElisabeteViegas, SusanaViegas, CarlaLaboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP)Centro de Investigação em Saúde Pública (CISP/PHRC)Comprehensive Health Research Centre (CHRC) - Pólo ENSPEscola Nacional de Saúde Pública (ENSP)Elsevier BVHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2666-1543URNIsPartOfPURE: 132905624URNIsPartOfPURE UUID: 2a13bd9f-9a14-4141-893e-5f9f98ec1949URNIsPartOfScopus: 105017263860URNIsPartOfWOS: 001588128700002DOIIsPartOf10.1016/j.jafr.2025.1023992025-10-23T21:58:12Z2025-122025-12-01T00:00:00ZHandlehttp://hdl.handle.net/10362/189670http://purl.org/coar/access_right/c_abf2open accessFood safetyGrowth performanceMycotoxinsPoultry productionToxigenic fungiFood ScienceAgronomy and Crop ScienceAgricultural and Biological Sciences (miscellaneous)SDG 3 - Good Health and Well-beingSDG 13 - Climate Action1676768 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/affcf702-ed61-4a13-a138-b441345ee68d/download
spellingShingle Toxicogenic fungi and mycotoxins seasonality in poultry farms
Gomes, Bianca
Food safety
Growth performance
Mycotoxins
Poultry production
Toxigenic fungi
Food Science
Agronomy and Crop Science
Agricultural and Biological Sciences (miscellaneous)
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
status SINGLETON
subject.fl_str_mv Food safety
Growth performance
Mycotoxins
Poultry production
Toxigenic fungi
Food Science
Agronomy and Crop Science
Agricultural and Biological Sciences (miscellaneous)
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
title Toxicogenic fungi and mycotoxins seasonality in poultry farms
title_full Toxicogenic fungi and mycotoxins seasonality in poultry farms
title_fullStr Toxicogenic fungi and mycotoxins seasonality in poultry farms
title_full_unstemmed Toxicogenic fungi and mycotoxins seasonality in poultry farms
title_short Toxicogenic fungi and mycotoxins seasonality in poultry farms
title_sort Toxicogenic fungi and mycotoxins seasonality in poultry farms
topic Food safety
Growth performance
Mycotoxins
Poultry production
Toxigenic fungi
Food Science
Agronomy and Crop Science
Agricultural and Biological Sciences (miscellaneous)
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
topic_facet Food safety
Growth performance
Mycotoxins
Poultry production
Toxigenic fungi
Food Science
Agronomy and Crop Science
Agricultural and Biological Sciences (miscellaneous)
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
url http://hdl.handle.net/10362/189670
visible 1