Publicação
Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia
| Resumo: | Background: Chagas disease is an emergent tropical disease in the Brazilian Amazon Region, with an increasing number of cases in recent decades. In this region, the sylvatic cycle of Trypanosoma cruzi transmission, which constitutes a reservoir of parasites that might be associated with specific molecular, epidemiological and clinical traits, has been little explored. The objective of this work is to genetically characterize stocks of T. cruzi from human cases, triatomines and reservoir mammals in the State of Amazonas, in the Western Brazilian Amazon. Methodology/Principal Findings: We analyzed 96 T. cruzi samples from four municipalities in distant locations of the State of Amazonas. Molecular characterization of isolated parasites from cultures in LIT medium or directly from vectors or whole human blood was performed by PCR of the non-transcribed spacer of the mini-exon and of the 24 S alfa ribosomal RNA gene, RFLP and sequencing of the mitochondrial cytochrome c oxidase subunit II (COII) gene, and by sequencing of the glucose-phosphate isomerase gene. The T. cruzi parasites from two outbreaks of acute disease were all typed as TcIV. One of the outbreaks was triggered by several haplotypes of the same DTU. TcIV also occurred in isolated cases and in Rhodnius robustus. Incongruence between mitochondrial and nuclear phylogenies is likely to be indicative of historical genetic exchange events resulting in mitochondrial introgression between TcIII and TcIV DTUs from Western Brazilian Amazon. TcI predominated among triatomines and was the unique DTU infecting marsupials. Conclusion/Significance: DTU TcIV, rarely associated with human Chagas disease in other areas of the Amazon basin, is the major strain responsible for the human infections in the Western Brazilian Amazon, occurring in outbreaks as single or mixed infections by different haplotypes. |
|---|---|
| Autores principais: | Monteiro, Wuelton Marcelo |
| Outros Autores: | Magalhães, Laylah Kelre Costa; de Sá, Amanda Regina Nichi; Gomes, Mônica Lúcia; Toledo, Max Jean de Ornelas; Borges, Lara; Pires, Isa; Guerra, Jorge Augusto de Oliveira; Silveira, Henrique; Barbosa, Maria das Graças Vale |
| Assunto: | Parasitology Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983724419317760 |
|---|---|
| author | Monteiro, Wuelton Marcelo |
| author2 | Magalhães, Laylah Kelre Costa de Sá, Amanda Regina Nichi Gomes, Mônica Lúcia Toledo, Max Jean de Ornelas Borges, Lara Pires, Isa Guerra, Jorge Augusto de Oliveira Silveira, Henrique Barbosa, Maria das Graças Vale |
| author2_role | author author author author author author author author author |
| author_facet | Monteiro, Wuelton Marcelo Magalhães, Laylah Kelre Costa de Sá, Amanda Regina Nichi Gomes, Mônica Lúcia Toledo, Max Jean de Ornelas Borges, Lara Pires, Isa Guerra, Jorge Augusto de Oliveira Silveira, Henrique Barbosa, Maria das Graças Vale |
| author_role | author |
| contributor_name_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) PLOS - Public Library of Science RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Monteiro, Wuelton Marcelo\"},{\"Person.name\":\"Magalhães, Laylah Kelre Costa\"},{\"Person.name\":\"de Sá, Amanda Regina Nichi\"},{\"Person.name\":\"Gomes, Mônica Lúcia\"},{\"Person.name\":\"Toledo, Max Jean de Ornelas\"},{\"Person.name\":\"Borges, Lara\"},{\"Person.name\":\"Pires, Isa\"},{\"Person.name\":\"Guerra, Jorge Augusto de Oliveira\"},{\"Person.name\":\"Silveira, Henrique\"},{\"Person.name\":\"Barbosa, Maria das Graças Vale\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) PLOS - Public Library of Science RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Monteiro, Wuelton Marcelo Magalhães, Laylah Kelre Costa de Sá, Amanda Regina Nichi Gomes, Mônica Lúcia Toledo, Max Jean de Ornelas Borges, Lara Pires, Isa Guerra, Jorge Augusto de Oliveira Silveira, Henrique Barbosa, Maria das Graças Vale |
| datacite.date.Accepted.fl_str_mv | 2012-07-25T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-05-05T23:25:33Z |
| datacite.date.embargoed.fl_str_mv | 2021-05-05T23:25:33Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Parasitology Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| dc.contributor.none.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) PLOS - Public Library of Science RUN |
| dc.creator.none.fl_str_mv | Monteiro, Wuelton Marcelo Magalhães, Laylah Kelre Costa de Sá, Amanda Regina Nichi Gomes, Mônica Lúcia Toledo, Max Jean de Ornelas Borges, Lara Pires, Isa Guerra, Jorge Augusto de Oliveira Silveira, Henrique Barbosa, Maria das Graças Vale |
| dc.date.Accepted.fl_str_mv | 2012-07-25T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-05-05T23:25:33Z |
| dc.date.embargoed.fl_str_mv | 2021-05-05T23:25:33Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/117143 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Parasitology Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: Chagas disease is an emergent tropical disease in the Brazilian Amazon Region, with an increasing number of cases in recent decades. In this region, the sylvatic cycle of Trypanosoma cruzi transmission, which constitutes a reservoir of parasites that might be associated with specific molecular, epidemiological and clinical traits, has been little explored. The objective of this work is to genetically characterize stocks of T. cruzi from human cases, triatomines and reservoir mammals in the State of Amazonas, in the Western Brazilian Amazon. Methodology/Principal Findings: We analyzed 96 T. cruzi samples from four municipalities in distant locations of the State of Amazonas. Molecular characterization of isolated parasites from cultures in LIT medium or directly from vectors or whole human blood was performed by PCR of the non-transcribed spacer of the mini-exon and of the 24 S alfa ribosomal RNA gene, RFLP and sequencing of the mitochondrial cytochrome c oxidase subunit II (COII) gene, and by sequencing of the glucose-phosphate isomerase gene. The T. cruzi parasites from two outbreaks of acute disease were all typed as TcIV. One of the outbreaks was triggered by several haplotypes of the same DTU. TcIV also occurred in isolated cases and in Rhodnius robustus. Incongruence between mitochondrial and nuclear phylogenies is likely to be indicative of historical genetic exchange events resulting in mitochondrial introgression between TcIII and TcIV DTUs from Western Brazilian Amazon. TcI predominated among triatomines and was the unique DTU infecting marsupials. Conclusion/Significance: DTU TcIV, rarely associated with human Chagas disease in other areas of the Amazon basin, is the major strain responsible for the human infections in the Western Brazilian Amazon, occurring in outbreaks as single or mixed infections by different haplotypes. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/21c70381-528a-4712-a42e-581c360398ac/download |
| id | run_9356cf103b8d6ef83523d4e2f4ef3b82 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/117143 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/117143 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Monteiro, Wuelton Marcelo Magalhães, Laylah Kelre Costa de Sá, Amanda Regina Nichi Gomes, Mônica Lúcia Toledo, Max Jean de Ornelas Borges, Lara Pires, Isa Guerra, Jorge Augusto de Oliveira Silveira, Henrique Barbosa, Maria das Graças Vale |
| publishDate | 2012 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
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| spelling | engenBackground: Chagas disease is an emergent tropical disease in the Brazilian Amazon Region, with an increasing number of cases in recent decades. In this region, the sylvatic cycle of Trypanosoma cruzi transmission, which constitutes a reservoir of parasites that might be associated with specific molecular, epidemiological and clinical traits, has been little explored. The objective of this work is to genetically characterize stocks of T. cruzi from human cases, triatomines and reservoir mammals in the State of Amazonas, in the Western Brazilian Amazon. Methodology/Principal Findings: We analyzed 96 T. cruzi samples from four municipalities in distant locations of the State of Amazonas. Molecular characterization of isolated parasites from cultures in LIT medium or directly from vectors or whole human blood was performed by PCR of the non-transcribed spacer of the mini-exon and of the 24 S alfa ribosomal RNA gene, RFLP and sequencing of the mitochondrial cytochrome c oxidase subunit II (COII) gene, and by sequencing of the glucose-phosphate isomerase gene. The T. cruzi parasites from two outbreaks of acute disease were all typed as TcIV. One of the outbreaks was triggered by several haplotypes of the same DTU. TcIV also occurred in isolated cases and in Rhodnius robustus. Incongruence between mitochondrial and nuclear phylogenies is likely to be indicative of historical genetic exchange events resulting in mitochondrial introgression between TcIII and TcIV DTUs from Western Brazilian Amazon. TcI predominated among triatomines and was the unique DTU infecting marsupials. Conclusion/Significance: DTU TcIV, rarely associated with human Chagas disease in other areas of the Amazon basin, is the major strain responsible for the human infections in the Western Brazilian Amazon, occurring in outbreaks as single or mixed infections by different haplotypes.application/pdfenTrypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian AmazoniaMonteiro, Wuelton MarceloMagalhães, Laylah Kelre Costade Sá, Amanda Regina NichiGomes, Mônica LúciaToledo, Max Jean de OrnelasBorges, LaraPires, IsaGuerra, Jorge Augusto de OliveiraSilveira, HenriqueBarbosa, Maria das Graças ValeInstituto de Higiene e Medicina Tropical (IHMT)Centro de Malária e outras Doenças Tropicais (CMDT)PLOS - Public Library of ScienceHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1932-6203URNIsPartOfPURE: 26755663URNIsPartOfPURE UUID: f60faf18-1445-4ed0-9506-55dd0ef236edURNIsPartOfScopus: 84864488817URNIsPartOfPubMed: 22848457URNIsPartOfWOS: 000306806600049URNIsPartOfORCID: /0000-0002-7939-772X/work/84883116DOIIsPartOf10.1371/journal.pone.00412842021-05-05T23:25:33Z2012-07-252012-07-25T00:00:00ZHandlehttp://hdl.handle.net/10362/117143http://purl.org/coar/access_right/c_abf2open accessParasitologyInfectious DiseasesEpidemiologySDG 3 - Good Health and Well-being433117 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/21c70381-528a-4712-a42e-581c360398ac/download |
| spellingShingle | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia Monteiro, Wuelton Marcelo Parasitology Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | Parasitology Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
| title | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| title_full | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| title_fullStr | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| title_full_unstemmed | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| title_short | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| title_sort | Trypanosoma cruzi IV causing outbreaks of acute chagas disease and infections by different haplotypes in the Western Brazilian Amazonia |
| topic | Parasitology Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
| topic_facet | Parasitology Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/117143 |
| visible | 1 |