Publicação
Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018
| Resumo: | The prevalence and distribution of African alphaviruses such as chikungunya have increased in recent years. Therefore, a better understanding of the local distribution of alphaviruses in vectors across the African continent is important. Here, entomological surveillance was performed from 2014 to 2018 at selected sites in north-eastern parts of South Africa where alphaviruses have been identified during outbreaks in humans and animals in the past. Mosquitoes were collected using a net, CDC-light, and BG-traps. An alphavirus genus-specific nested RT-PCR was used for screening, and positive pools were confirmed by sequencing and phylogenetic analysis. We collected 64,603 mosquitoes from 11 genera, of which 39,035 females were tested. Overall, 1462 mosquito pools were tested, of which 21 were positive for alphaviruses. Sindbis (61.9%, N = 13) and Middelburg (28.6%, N = 6) viruses were the most prevalent. Ndumu virus was detected in two pools (9.5%, N = 2). No chikungunya positive pools were identified. Arboviral activity was concentrated in peri-urban, rural, and conservation areas. A range of Culicidae species, including Culex univittatus, Cx. pipiens s.l., Aedes durbanensis, and the Ae. dentatus group, were identified as potential vectors. These findings confirm the active circulation and distribution of alphaviruses in regions where human or animal infections were identified in South Africa. |
|---|---|
| Autores principais: | Guarido, Milehna Mara |
| Outros Autores: | Fourie, Isabel; Meno, Kgothatso; Mendes, Adriano; Riddin, Megan A.; MacIntyre, Caitlin; Manyana, Sontaga Cris; Johnson, Todd; Schrama, Maarten; Gorsich, Erin E.; Brooke, Basil D.; Almeida, A.P.G.; Venter, Marietjie |
| Assunto: | alphaviruses Middelburg Sindbis Ndumu Mosquitoes Aedes Culex Africa region QR355 Virology RA0421 Public health. Hygiene. Preventive Medicine T Technology Molecular Biology Virology Epidemiology Insect Science SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 15 - Life on Land |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868982784334233600 |
|---|---|
| author | Guarido, Milehna Mara |
| author2 | Fourie, Isabel Meno, Kgothatso Mendes, Adriano Riddin, Megan A. MacIntyre, Caitlin Manyana, Sontaga Cris Johnson, Todd Schrama, Maarten Gorsich, Erin E. Brooke, Basil D. Almeida, A.P.G. Venter, Marietjie |
| author2_role | author author author author author author author author author author author author |
| author_facet | Guarido, Milehna Mara Fourie, Isabel Meno, Kgothatso Mendes, Adriano Riddin, Megan A. MacIntyre, Caitlin Manyana, Sontaga Cris Johnson, Todd Schrama, Maarten Gorsich, Erin E. Brooke, Basil D. Almeida, A.P.G. Venter, Marietjie |
| author_role | author |
| contributor_name_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Guarido, Milehna Mara\"},{\"Person.name\":\"Fourie, Isabel\"},{\"Person.name\":\"Meno, Kgothatso\"},{\"Person.name\":\"Mendes, Adriano\"},{\"Person.name\":\"Riddin, Megan A.\"},{\"Person.name\":\"MacIntyre, Caitlin\"},{\"Person.name\":\"Manyana, Sontaga Cris\"},{\"Person.name\":\"Johnson, Todd\"},{\"Person.name\":\"Schrama, Maarten\"},{\"Person.name\":\"Gorsich, Erin E.\"},{\"Person.name\":\"Brooke, Basil D.\"},{\"Person.name\":\"Almeida, A.P.G.\"},{\"Person.name\":\"Venter, Marietjie\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Guarido, Milehna Mara Fourie, Isabel Meno, Kgothatso Mendes, Adriano Riddin, Megan A. MacIntyre, Caitlin Manyana, Sontaga Cris Johnson, Todd Schrama, Maarten Gorsich, Erin E. Brooke, Basil D. Almeida, A.P.G. Venter, Marietjie |
| datacite.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-03T22:25:05Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-03T22:25:05Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | alphaviruses Middelburg Sindbis Ndumu Mosquitoes Aedes Culex Africa region QR355 Virology RA0421 Public health. Hygiene. Preventive Medicine T Technology Molecular Biology Virology Epidemiology Insect Science SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 15 - Life on Land |
| datacite.titles.title.fl_str_mv | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| dc.contributor.none.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) MDPI - Multidisciplinary Digital Publishing Institute RUN |
| dc.creator.none.fl_str_mv | Guarido, Milehna Mara Fourie, Isabel Meno, Kgothatso Mendes, Adriano Riddin, Megan A. MacIntyre, Caitlin Manyana, Sontaga Cris Johnson, Todd Schrama, Maarten Gorsich, Erin E. Brooke, Basil D. Almeida, A.P.G. Venter, Marietjie |
| dc.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-03T22:25:05Z |
| dc.date.embargoed.fl_str_mv | 2023-03-03T22:25:05Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/149981 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | alphaviruses Middelburg Sindbis Ndumu Mosquitoes Aedes Culex Africa region QR355 Virology RA0421 Public health. Hygiene. Preventive Medicine T Technology Molecular Biology Virology Epidemiology Insect Science SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 15 - Life on Land |
| dc.title.fl_str_mv | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The prevalence and distribution of African alphaviruses such as chikungunya have increased in recent years. Therefore, a better understanding of the local distribution of alphaviruses in vectors across the African continent is important. Here, entomological surveillance was performed from 2014 to 2018 at selected sites in north-eastern parts of South Africa where alphaviruses have been identified during outbreaks in humans and animals in the past. Mosquitoes were collected using a net, CDC-light, and BG-traps. An alphavirus genus-specific nested RT-PCR was used for screening, and positive pools were confirmed by sequencing and phylogenetic analysis. We collected 64,603 mosquitoes from 11 genera, of which 39,035 females were tested. Overall, 1462 mosquito pools were tested, of which 21 were positive for alphaviruses. Sindbis (61.9%, N = 13) and Middelburg (28.6%, N = 6) viruses were the most prevalent. Ndumu virus was detected in two pools (9.5%, N = 2). No chikungunya positive pools were identified. Arboviral activity was concentrated in peri-urban, rural, and conservation areas. A range of Culicidae species, including Culex univittatus, Cx. pipiens s.l., Aedes durbanensis, and the Ae. dentatus group, were identified as potential vectors. These findings confirm the active circulation and distribution of alphaviruses in regions where human or animal infections were identified in South Africa. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/f07ecf15-8a46-47d8-9abb-9c801ab1b20b/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10362/149981 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
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| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
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| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/149981 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Guarido, Milehna Mara Fourie, Isabel Meno, Kgothatso Mendes, Adriano Riddin, Megan A. MacIntyre, Caitlin Manyana, Sontaga Cris Johnson, Todd Schrama, Maarten Gorsich, Erin E. Brooke, Basil D. Almeida, A.P.G. Venter, Marietjie |
| publishDate | 2023 |
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| reponame_str | Repositório Institucional da UNL |
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| spelling | engenThe prevalence and distribution of African alphaviruses such as chikungunya have increased in recent years. Therefore, a better understanding of the local distribution of alphaviruses in vectors across the African continent is important. Here, entomological surveillance was performed from 2014 to 2018 at selected sites in north-eastern parts of South Africa where alphaviruses have been identified during outbreaks in humans and animals in the past. Mosquitoes were collected using a net, CDC-light, and BG-traps. An alphavirus genus-specific nested RT-PCR was used for screening, and positive pools were confirmed by sequencing and phylogenetic analysis. We collected 64,603 mosquitoes from 11 genera, of which 39,035 females were tested. Overall, 1462 mosquito pools were tested, of which 21 were positive for alphaviruses. Sindbis (61.9%, N = 13) and Middelburg (28.6%, N = 6) viruses were the most prevalent. Ndumu virus was detected in two pools (9.5%, N = 2). No chikungunya positive pools were identified. Arboviral activity was concentrated in peri-urban, rural, and conservation areas. A range of Culicidae species, including Culex univittatus, Cx. pipiens s.l., Aedes durbanensis, and the Ae. dentatus group, were identified as potential vectors. These findings confirm the active circulation and distribution of alphaviruses in regions where human or animal infections were identified in South Africa.application/pdfenAlphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018Guarido, Milehna MaraFourie, IsabelMeno, KgothatsoMendes, AdrianoRiddin, Megan A.MacIntyre, CaitlinManyana, Sontaga CrisJohnson, ToddSchrama, MaartenGorsich, Erin E.Brooke, Basil D.Almeida, A.P.G.Venter, MarietjieInstituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)MDPI - Multidisciplinary Digital Publishing InstituteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1999-4915URNIsPartOfPURE: 52764762URNIsPartOfPURE UUID: 6cd506a7-9615-4d33-8d5a-c219445c5803URNIsPartOfPubMedCentral: PMC9965626URNIsPartOfPubMed: 36851627URNIsPartOfORCID: /0000-0003-0751-4488/work/130153597URNIsPartOfWOS: 000941994200001URNIsPartOfScopus: 85149202041DOIIsPartOf10.3390/v150204142023-03-03T22:25:05Z20232023-01-01T00:00:00ZHandlehttp://hdl.handle.net/10362/149981http://purl.org/coar/access_right/c_abf2open accessalphavirusesMiddelburgSindbisNdumuMosquitoesAedesCulexAfrica regionQR355 VirologyRA0421 Public health. Hygiene. Preventive MedicineT TechnologyMolecular BiologyVirologyEpidemiologyInsect ScienceSDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureSDG 15 - Life on Land3449980 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/f07ecf15-8a46-47d8-9abb-9c801ab1b20b/download |
| spellingShingle | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 Guarido, Milehna Mara alphaviruses Middelburg Sindbis Ndumu Mosquitoes Aedes Culex Africa region QR355 Virology RA0421 Public health. Hygiene. Preventive Medicine T Technology Molecular Biology Virology Epidemiology Insect Science SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 15 - Life on Land |
| status | SINGLETON |
| subject.fl_str_mv | alphaviruses Middelburg Sindbis Ndumu Mosquitoes Aedes Culex Africa region QR355 Virology RA0421 Public health. Hygiene. Preventive Medicine T Technology Molecular Biology Virology Epidemiology Insect Science SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 15 - Life on Land |
| title | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| title_full | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| title_fullStr | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| title_full_unstemmed | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| title_short | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| title_sort | Alphaviruses Detected in Mosquitoes in the North-Eastern Regions of South Africa, 2014 to 2018 |
| topic | alphaviruses Middelburg Sindbis Ndumu Mosquitoes Aedes Culex Africa region QR355 Virology RA0421 Public health. Hygiene. Preventive Medicine T Technology Molecular Biology Virology Epidemiology Insect Science SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 15 - Life on Land |
| topic_facet | alphaviruses Middelburg Sindbis Ndumu Mosquitoes Aedes Culex Africa region QR355 Virology RA0421 Public health. Hygiene. Preventive Medicine T Technology Molecular Biology Virology Epidemiology Insect Science SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 15 - Life on Land |
| url | http://hdl.handle.net/10362/149981 |
| visible | 1 |