Publicação
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy
| Resumo: | Early-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who underwent intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. Initial effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria number (+21%, p = 0.026), a decrease in Staphylococcus (−21%, p = 0.002) and Candida spp. (−16%, p = 0.045), and an increase in Lactobacillus (+3%, p = 0.045) and in less abundant genera belonging to the Enterobacteriales family were found. An increase in alpha diversity (Shannon’s and Simpson’s indexes) and significant alterations in beta diversity were observed. A correlation of necrotizing enterocolitis with higher Staphylococcus abundance and higher alpha diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria number. In conclusion, these results suggest that positive changes occur in the gut microbiota profile of infants three weeks after intestinal surgery. |
|---|---|
| Autores principais: | Barreiros-Mota, Inês |
| Outros Autores: | R. Araújo, João; Marques, Cláudia; Sousa, Laura; Morais, Juliana; Castela, Inês; Faria, Ana; Neto, Maria Teresa; Cordeiro-Ferreira, Gonçalo; Virella, Daniel; Pita, Ana; Pereira-da-Silva, Luís; Pereira-da-Silva, Luís; Calhau, Conceição; Calhau, Conceição |
| Assunto: | enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983997494722560 |
|---|---|
| author | Barreiros-Mota, Inês |
| author2 | R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Pereira-da-Silva, Luís Calhau, Conceição Calhau, Conceição |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Barreiros-Mota, Inês R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Pereira-da-Silva, Luís Calhau, Conceição Calhau, Conceição |
| author_role | author |
| contributor_name_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Comprehensive Health Research Centre (CHRC) - pólo NMS Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS) MDPI AG RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Barreiros-Mota, Inês\"},{\"Person.name\":\"R. Araújo, João\"},{\"Person.name\":\"Marques, Cláudia\"},{\"Person.name\":\"Sousa, Laura\"},{\"Person.name\":\"Morais, Juliana\"},{\"Person.name\":\"Castela, Inês\"},{\"Person.name\":\"Faria, Ana\"},{\"Person.name\":\"Neto, Maria Teresa\"},{\"Person.name\":\"Cordeiro-Ferreira, Gonçalo\"},{\"Person.name\":\"Virella, Daniel\"},{\"Person.name\":\"Pita, Ana\"},{\"Person.name\":\"Pereira-da-Silva, Luís\"},{\"Person.name\":\"Pereira-da-Silva, Luís\",\"Person.identifier.orcid\":\"0000-0002-6480-1048\"},{\"Person.name\":\"Calhau, Conceição\"},{\"Person.name\":\"Calhau, Conceição\",\"Person.identifier.orcid\":\"0000-0001-9567-3379\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Comprehensive Health Research Centre (CHRC) - pólo NMS Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS) MDPI AG RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Barreiros-Mota, Inês R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Pereira-da-Silva, Luís Calhau, Conceição Calhau, Conceição |
| datacite.date.Accepted.fl_str_mv | 2023-10-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-11-30T22:19:57Z |
| datacite.date.embargoed.fl_str_mv | 2023-11-30T22:19:57Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| datacite.titles.title.fl_str_mv | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| dc.contributor.none.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Comprehensive Health Research Centre (CHRC) - pólo NMS Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS) MDPI AG RUN |
| dc.creator.none.fl_str_mv | Barreiros-Mota, Inês R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Pereira-da-Silva, Luís Calhau, Conceição Calhau, Conceição |
| dc.date.Accepted.fl_str_mv | 2023-10-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-11-30T22:19:57Z |
| dc.date.embargoed.fl_str_mv | 2023-11-30T22:19:57Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/160765 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| dc.title.fl_str_mv | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Early-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who underwent intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. Initial effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria number (+21%, p = 0.026), a decrease in Staphylococcus (−21%, p = 0.002) and Candida spp. (−16%, p = 0.045), and an increase in Lactobacillus (+3%, p = 0.045) and in less abundant genera belonging to the Enterobacteriales family were found. An increase in alpha diversity (Shannon’s and Simpson’s indexes) and significant alterations in beta diversity were observed. A correlation of necrotizing enterocolitis with higher Staphylococcus abundance and higher alpha diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria number. In conclusion, these results suggest that positive changes occur in the gut microbiota profile of infants three weeks after intestinal surgery. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/504e33be-6ff9-4047-8336-7e50b28e449c/download |
| id | run_aa9f4bdc4339a5c08e956c1c1934b5d4 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/160765 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/160765 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Barreiros-Mota, Inês R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Pereira-da-Silva, Luís Pereira-da-Silva, Luís https://www.ciencia-id.pt/001E-3D9B-A4CF 001E-3D9B-A4CF http://orcid.org/0000-0002-6480-1048 0000-0002-6480-1048 Calhau, Conceição Calhau, Conceição Calhau, Conceição https://www.ciencia-id.pt/2110-0420-1717 2110-0420-1717 http://orcid.org/0000-0001-9567-3379 0000-0001-9567-3379 |
| publishDate | 2023 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
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| spelling | engenEarly-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who underwent intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. Initial effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria number (+21%, p = 0.026), a decrease in Staphylococcus (−21%, p = 0.002) and Candida spp. (−16%, p = 0.045), and an increase in Lactobacillus (+3%, p = 0.045) and in less abundant genera belonging to the Enterobacteriales family were found. An increase in alpha diversity (Shannon’s and Simpson’s indexes) and significant alterations in beta diversity were observed. A correlation of necrotizing enterocolitis with higher Staphylococcus abundance and higher alpha diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria number. In conclusion, these results suggest that positive changes occur in the gut microbiota profile of infants three weeks after intestinal surgery.application/pdfenChanges in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring EnterostomyBarreiros-Mota, InêsR. Araújo, JoãoMarques, CláudiaSousa, LauraMorais, JulianaCastela, InêsFaria, AnaNeto, Maria TeresaCordeiro-Ferreira, GonçaloVirella, DanielPita, AnaPereira-da-Silva, LuísPersonalPereira-da-Silva, LuísDSpacehttp://dspace.org/items/69bc8408-2804-4ca3-a938-90b72cadda88DSpacehttp://dspace.org/items/69bc8408-2804-4ca3-a938-90b72cadda88Pereira-da-SilvaLuísCiência IDhttps://www.ciencia-id.pt001E-3D9B-A4CFORCIDhttp://orcid.org0000-0002-6480-1048Researcher IDhttps://www.researcherid.comA-3469-2008Calhau, ConceiçãoPersonalCalhau, ConceiçãoDSpacehttp://dspace.org/items/32b016c8-810a-40be-b9ee-ddfb07668a78DSpacehttp://dspace.org/items/32b016c8-810a-40be-b9ee-ddfb07668a78CalhauConceiçãoCiência IDhttps://www.ciencia-id.pt2110-0420-1717ORCIDhttp://orcid.org0000-0001-9567-3379Scopus Author IDhttps://www.scopus.com6701578877NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Comprehensive Health Research Centre (CHRC) - pólo NMSCentro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS)MDPI AGHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2076-2607URNIsPartOfPURE: 75293223URNIsPartOfPURE UUID: 774ba4d6-cfa6-4902-88c7-b340a835acf4URNIsPartOfScopus: 85175096102URNIsPartOfORCID: /0000-0003-2776-664X/work/151417101URNIsPartOfPubMed: 37894140DOIIsPartOf10.3390/microorganisms111024822023-11-30T22:19:57Z2023-102023-10-01T00:00:00ZHandlehttp://hdl.handle.net/10362/160765http://purl.org/coar/access_right/c_abf2open accessenterostomyinfantsmicrobiotanecrotizing enterocolitisprematurityMicrobiologyMicrobiology (medical)Virology2057466 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/504e33be-6ff9-4047-8336-7e50b28e449c/download |
| spellingShingle | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy Barreiros-Mota, Inês enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| status | SINGLETON |
| subject.fl_str_mv | enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| title | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_full | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_fullStr | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_full_unstemmed | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_short | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_sort | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| topic | enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| topic_facet | enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| url | http://hdl.handle.net/10362/160765 |
| visible | 1 |