Publication
PD-1 Expression in Endometriosis
| Summary: | Background: Endometriosis, believed by many to be rooted in immunology, is a chronic disease. Upregulation of programmed cell death protein 1 (PD-1) in immune cells may compromise their defensive function, a mechanism demonstrated in the context of cancer spread. This study aims to explore the potential involvement of PD-1 in the pathophysiology and progression of endometriosis. A total of 62 patients who underwent laparoscopic surgery were analyzed, with 47 diagnosed with endometriosis and 15 serving as controls. We collected peritoneal fluid and peripheral blood samples during surgery and examined them using flow cytometry. Using a panel of monoclonal antibodies, the samples were stained and the expression of PD-1 in immune cells was evaluated. Results: We observed a statistically significant rise in the percentage of the CD56+ CD16+ NK cell subset expressing PD-1 within the peritoneal fluid of endometriosis patients compared to the control group (p = 0.021). Similarly, we found that PD-1 expression on immune cells significantly differed based on factors such as body mass index and smoking habits. Moreover, peritoneal subsets of PD-1+ T and NK cells showed an increase in patients presenting symptomatic endometriosis and those with more widespread disease. Conclusions: Our evaluation of the inhibitory PD-1 receptor has strengthened the potential connection between immune escape mechanisms often seen in cancer cells and those in endometriotic cells. This concept could pave the way for future research in the field of immunomodulation and endometriosis. |
|---|---|
| Main Authors: | Reis, José Lourenço |
| Other Authors: | Martins, Catarina; Ângelo-Dias, Miguel; Rosa, Natacha Nurdine; Borrego, Luís Miguel; Lima, Jorge |
| Subject: | endometriosis immune receptors NK cells PD-1 T cells Medicine (miscellaneous) Immunology Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| Year: | 2025 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade Nova de Lisboa |
| Language: | English |
| Origin: | Repositório Institucional da UNL |
| _version_ | 1868983222839279616 |
|---|---|
| author | Reis, José Lourenço |
| author2 | Martins, Catarina Ângelo-Dias, Miguel Rosa, Natacha Nurdine Borrego, Luís Miguel Lima, Jorge |
| author2_role | author author author author author |
| author_facet | Reis, José Lourenço Martins, Catarina Ângelo-Dias, Miguel Rosa, Natacha Nurdine Borrego, Luís Miguel Lima, Jorge |
| author_role | author |
| contributor_name_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Comprehensive Health Research Centre (CHRC) - pólo NMS MDPI - Multidisciplinary Digital Publishing Institute RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Reis, José Lourenço\"},{\"Person.name\":\"Martins, Catarina\"},{\"Person.name\":\"Ângelo-Dias, Miguel\"},{\"Person.name\":\"Rosa, Natacha Nurdine\"},{\"Person.name\":\"Borrego, Luís Miguel\"},{\"Person.name\":\"Lima, Jorge\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Comprehensive Health Research Centre (CHRC) - pólo NMS MDPI - Multidisciplinary Digital Publishing Institute RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Reis, José Lourenço Martins, Catarina Ângelo-Dias, Miguel Rosa, Natacha Nurdine Borrego, Luís Miguel Lima, Jorge |
| datacite.date.Accepted.fl_str_mv | 2025-12-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-01-14T15:55:04Z |
| datacite.date.embargoed.fl_str_mv | 2026-01-14T15:55:04Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | endometriosis immune receptors NK cells PD-1 T cells Medicine (miscellaneous) Immunology Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | PD-1 Expression in Endometriosis |
| dc.contributor.none.fl_str_mv | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Comprehensive Health Research Centre (CHRC) - pólo NMS MDPI - Multidisciplinary Digital Publishing Institute RUN |
| dc.creator.none.fl_str_mv | Reis, José Lourenço Martins, Catarina Ângelo-Dias, Miguel Rosa, Natacha Nurdine Borrego, Luís Miguel Lima, Jorge |
| dc.date.Accepted.fl_str_mv | 2025-12-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-01-14T15:55:04Z |
| dc.date.embargoed.fl_str_mv | 2026-01-14T15:55:04Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/199055 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | endometriosis immune receptors NK cells PD-1 T cells Medicine (miscellaneous) Immunology Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | PD-1 Expression in Endometriosis |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: Endometriosis, believed by many to be rooted in immunology, is a chronic disease. Upregulation of programmed cell death protein 1 (PD-1) in immune cells may compromise their defensive function, a mechanism demonstrated in the context of cancer spread. This study aims to explore the potential involvement of PD-1 in the pathophysiology and progression of endometriosis. A total of 62 patients who underwent laparoscopic surgery were analyzed, with 47 diagnosed with endometriosis and 15 serving as controls. We collected peritoneal fluid and peripheral blood samples during surgery and examined them using flow cytometry. Using a panel of monoclonal antibodies, the samples were stained and the expression of PD-1 in immune cells was evaluated. Results: We observed a statistically significant rise in the percentage of the CD56+ CD16+ NK cell subset expressing PD-1 within the peritoneal fluid of endometriosis patients compared to the control group (p = 0.021). Similarly, we found that PD-1 expression on immune cells significantly differed based on factors such as body mass index and smoking habits. Moreover, peritoneal subsets of PD-1+ T and NK cells showed an increase in patients presenting symptomatic endometriosis and those with more widespread disease. Conclusions: Our evaluation of the inhibitory PD-1 receptor has strengthened the potential connection between immune escape mechanisms often seen in cancer cells and those in endometriotic cells. This concept could pave the way for future research in the field of immunomodulation and endometriosis. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/598684cb-5404-44a2-874e-5fff90663df5/download |
| id | run_bc070fbc25d12166cd835dba3f80e2fd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/199055 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/199055 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Reis, José Lourenço Martins, Catarina Ângelo-Dias, Miguel Rosa, Natacha Nurdine Borrego, Luís Miguel Lima, Jorge |
| publishDate | 2025 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
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| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenBackground: Endometriosis, believed by many to be rooted in immunology, is a chronic disease. Upregulation of programmed cell death protein 1 (PD-1) in immune cells may compromise their defensive function, a mechanism demonstrated in the context of cancer spread. This study aims to explore the potential involvement of PD-1 in the pathophysiology and progression of endometriosis. A total of 62 patients who underwent laparoscopic surgery were analyzed, with 47 diagnosed with endometriosis and 15 serving as controls. We collected peritoneal fluid and peripheral blood samples during surgery and examined them using flow cytometry. Using a panel of monoclonal antibodies, the samples were stained and the expression of PD-1 in immune cells was evaluated. Results: We observed a statistically significant rise in the percentage of the CD56+ CD16+ NK cell subset expressing PD-1 within the peritoneal fluid of endometriosis patients compared to the control group (p = 0.021). Similarly, we found that PD-1 expression on immune cells significantly differed based on factors such as body mass index and smoking habits. Moreover, peritoneal subsets of PD-1+ T and NK cells showed an increase in patients presenting symptomatic endometriosis and those with more widespread disease. Conclusions: Our evaluation of the inhibitory PD-1 receptor has strengthened the potential connection between immune escape mechanisms often seen in cancer cells and those in endometriotic cells. This concept could pave the way for future research in the field of immunomodulation and endometriosis.application/pdfenPD-1 Expression in EndometriosisReis, José LourençoMartins, CatarinaÂngelo-Dias, MiguelRosa, Natacha NurdineBorrego, Luís MiguelLima, JorgeNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Comprehensive Health Research Centre (CHRC) - pólo NMSMDPI - Multidisciplinary Digital Publishing InstituteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2673-5601URNIsPartOfPURE: 148988362URNIsPartOfPURE UUID: 2f1a244b-5d2a-461a-a859-da1e2c71f840URNIsPartOfScopus: 105025714360URNIsPartOfORCID: /0000-0003-0353-0421/work/202237336URNIsPartOfORCID: /0000-0003-4708-438X/work/202237738URNIsPartOfORCID: /0000-0001-9933-4075/work/206216936URNIsPartOfWOS: 001646673300001DOIIsPartOf10.3390/immuno50400492026-01-14T15:55:04Z2025-122025-12-01T00:00:00ZHandlehttp://hdl.handle.net/10362/199055http://purl.org/coar/access_right/c_abf2open accessendometriosisimmune receptorsNK cellsPD-1T cellsMedicine (miscellaneous)ImmunologyBiochemistry, Genetics and Molecular Biology (miscellaneous)SDG 3 - Good Health and Well-being2893600 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/598684cb-5404-44a2-874e-5fff90663df5/download |
| spellingShingle | PD-1 Expression in Endometriosis Reis, José Lourenço endometriosis immune receptors NK cells PD-1 T cells Medicine (miscellaneous) Immunology Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | endometriosis immune receptors NK cells PD-1 T cells Medicine (miscellaneous) Immunology Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| title | PD-1 Expression in Endometriosis |
| title_full | PD-1 Expression in Endometriosis |
| title_fullStr | PD-1 Expression in Endometriosis |
| title_full_unstemmed | PD-1 Expression in Endometriosis |
| title_short | PD-1 Expression in Endometriosis |
| title_sort | PD-1 Expression in Endometriosis |
| topic | endometriosis immune receptors NK cells PD-1 T cells Medicine (miscellaneous) Immunology Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| topic_facet | endometriosis immune receptors NK cells PD-1 T cells Medicine (miscellaneous) Immunology Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/199055 |
| visible | 1 |