Publicação
Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1
| Resumo: | The contribution of Fe(II)-oxidizing bacteria to iron cycling in freshwater, groundwater, and marine environments has been widely recognized in recent years. These organisms perform extracellular electron transfer (EET), which constitutes the foundations of bioelectrochemical systems for the production of biofuels and bioenergy. It was proposed that the Gram-negative bacterium Sideroxydans lithotrophicus ES-1 oxidizes soluble ferrous Fe(II) at the surface of the cell and performs EET through the Mto redox pathway. This pathway is composed by the periplasmic monoheme cytochrome MtoD that is proposed to bridge electron transfer between the cell exterior and the cytoplasm. This makes its functional and structural characterization, as well as evaluating the interaction process with its physiological partners, essential for understanding the mechanisms underlying EET. Here, we report the complete assignment of the heme proton and carbon signals together with a near-complete assignment of 1H, 13C and 15N backbone and side chain resonances for the reduced, diamagnetic form of the protein. These data pave the way to identify and structurally map the molecular interaction regions between the cytochrome MtoD and its physiological redox partners, to explore the EET processes of S. lithotrophicus ES-1. |
|---|---|
| Autores principais: | Coelho, Anaísa |
| Outros Autores: | Silva, José M.; Cantini, Francesca; Piccioli, Mario; Louro, Ricardo O.; Paquete, Catarina M. |
| Assunto: | Cytochromes Extracellular electron transfer MtoD NMR resonance assignment Ring current shifts Structural Biology Biochemistry SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868984103356858368 |
|---|---|
| author | Coelho, Anaísa |
| author2 | Silva, José M. Cantini, Francesca Piccioli, Mario Louro, Ricardo O. Paquete, Catarina M. |
| author2_role | author author author author author |
| author_facet | Coelho, Anaísa Silva, José M. Cantini, Francesca Piccioli, Mario Louro, Ricardo O. Paquete, Catarina M. |
| author_role | author |
| contributor_name_str_mv | Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Springer RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Coelho, Anaísa\"},{\"Person.name\":\"Silva, José M.\"},{\"Person.name\":\"Cantini, Francesca\"},{\"Person.name\":\"Piccioli, Mario\"},{\"Person.name\":\"Louro, Ricardo O.\"},{\"Person.name\":\"Paquete, Catarina M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Springer RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Coelho, Anaísa Silva, José M. Cantini, Francesca Piccioli, Mario Louro, Ricardo O. Paquete, Catarina M. |
| datacite.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-05-07T21:19:24Z |
| datacite.date.embargoed.fl_str_mv | 2025-05-07T21:19:24Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cytochromes Extracellular electron transfer MtoD NMR resonance assignment Ring current shifts Structural Biology Biochemistry SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| datacite.titles.title.fl_str_mv | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| dc.contributor.none.fl_str_mv | Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Springer RUN |
| dc.creator.none.fl_str_mv | Coelho, Anaísa Silva, José M. Cantini, Francesca Piccioli, Mario Louro, Ricardo O. Paquete, Catarina M. |
| dc.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-05-07T21:19:24Z |
| dc.date.embargoed.fl_str_mv | 2025-05-07T21:19:24Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/182746 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cytochromes Extracellular electron transfer MtoD NMR resonance assignment Ring current shifts Structural Biology Biochemistry SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| dc.title.fl_str_mv | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The contribution of Fe(II)-oxidizing bacteria to iron cycling in freshwater, groundwater, and marine environments has been widely recognized in recent years. These organisms perform extracellular electron transfer (EET), which constitutes the foundations of bioelectrochemical systems for the production of biofuels and bioenergy. It was proposed that the Gram-negative bacterium Sideroxydans lithotrophicus ES-1 oxidizes soluble ferrous Fe(II) at the surface of the cell and performs EET through the Mto redox pathway. This pathway is composed by the periplasmic monoheme cytochrome MtoD that is proposed to bridge electron transfer between the cell exterior and the cytoplasm. This makes its functional and structural characterization, as well as evaluating the interaction process with its physiological partners, essential for understanding the mechanisms underlying EET. Here, we report the complete assignment of the heme proton and carbon signals together with a near-complete assignment of 1H, 13C and 15N backbone and side chain resonances for the reduced, diamagnetic form of the protein. These data pave the way to identify and structurally map the molecular interaction regions between the cytochrome MtoD and its physiological redox partners, to explore the EET processes of S. lithotrophicus ES-1. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/417594bd-0ed6-4db2-88e4-75aa75605780/download |
| id | run_c1bf455ea3279ced0232dd4e3d064eb9 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/182746 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/182746 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Coelho, Anaísa Silva, José M. Cantini, Francesca Piccioli, Mario Louro, Ricardo O. Paquete, Catarina M. |
| publishDate | 2024 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenThe contribution of Fe(II)-oxidizing bacteria to iron cycling in freshwater, groundwater, and marine environments has been widely recognized in recent years. These organisms perform extracellular electron transfer (EET), which constitutes the foundations of bioelectrochemical systems for the production of biofuels and bioenergy. It was proposed that the Gram-negative bacterium Sideroxydans lithotrophicus ES-1 oxidizes soluble ferrous Fe(II) at the surface of the cell and performs EET through the Mto redox pathway. This pathway is composed by the periplasmic monoheme cytochrome MtoD that is proposed to bridge electron transfer between the cell exterior and the cytoplasm. This makes its functional and structural characterization, as well as evaluating the interaction process with its physiological partners, essential for understanding the mechanisms underlying EET. Here, we report the complete assignment of the heme proton and carbon signals together with a near-complete assignment of 1H, 13C and 15N backbone and side chain resonances for the reduced, diamagnetic form of the protein. These data pave the way to identify and structurally map the molecular interaction regions between the cytochrome MtoD and its physiological redox partners, to explore the EET processes of S. lithotrophicus ES-1.application/pdfenResonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1Coelho, AnaísaSilva, José M.Cantini, FrancescaPiccioli, MarioLouro, Ricardo O.Paquete, Catarina M.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)SpringerHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1874-2718URNIsPartOfPURE: 99908831URNIsPartOfPURE UUID: 3908f254-7bb1-4e4a-963a-edd52c59a601URNIsPartOfScopus: 85195255574DOIIsPartOf10.1007/s12104-024-10180-82025-05-07T21:19:24Z20242024-01-01T00:00:00ZHandlehttp://hdl.handle.net/10362/182746http://purl.org/coar/access_right/c_abf2open accessCytochromesExtracellular electron transferMtoDNMR resonance assignmentRing current shiftsStructural BiologyBiochemistrySDG 6 - Clean Water and SanitationSDG 14 - Life Below Water1468792 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/417594bd-0ed6-4db2-88e4-75aa75605780/download |
| spellingShingle | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 Coelho, Anaísa Cytochromes Extracellular electron transfer MtoD NMR resonance assignment Ring current shifts Structural Biology Biochemistry SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| status | SINGLETON |
| subject.fl_str_mv | Cytochromes Extracellular electron transfer MtoD NMR resonance assignment Ring current shifts Structural Biology Biochemistry SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| title | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| title_full | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| title_fullStr | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| title_full_unstemmed | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| title_short | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| title_sort | Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1 |
| topic | Cytochromes Extracellular electron transfer MtoD NMR resonance assignment Ring current shifts Structural Biology Biochemistry SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| topic_facet | Cytochromes Extracellular electron transfer MtoD NMR resonance assignment Ring current shifts Structural Biology Biochemistry SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| url | http://hdl.handle.net/10362/182746 |
| visible | 1 |