Publicação
Sperm telomere length in patients undergoing infertility treatments: prospective study
| Resumo: | In recent years, studies have demonstrated the shortening of sperm telomeres (STL) in infertile patients, which has been associated with decreased chromatin concentration and compaction, increased DNA fragmentation (sDNAfrag) and aneuploidy in sperm. However, there are studies that contradict these observations. The main purpose of this project was to clarify whether STL is related to sperm quality and its clinical outcomes. For that, we evaluated the STL of patients undergoing infertility treatments and analyzed its relationship with all the seminal parameters, chromatin maturity, sDNAfrag, aneuploidies and oxidative profile, as well as the clinical parameters after the treatments, using swim-up purified spermatozoa. No statistically significant differences were observed in relation to the seminal parameters in the population of purified spermatozoa, suggesting the importance of this method of selection for clinical use. Another original aspect was the observation that the population with the highest STL is reduced, but it has a higher rate of pregnancy (40%), and that the majority of the population is located in an STL range in which the rate of pregnancy is lower (29 %). In future studies we intend to increase the sample size to consolidate the obtained results and to complement them with additional methods. |
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| Autores principais: | Lopes, Ana Catarina Vieira |
| Assunto: | sperm telomeres human reproduction male infertility assisted reproductive technology |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| Resumo: | In recent years, studies have demonstrated the shortening of sperm telomeres (STL) in infertile patients, which has been associated with decreased chromatin concentration and compaction, increased DNA fragmentation (sDNAfrag) and aneuploidy in sperm. However, there are studies that contradict these observations. The main purpose of this project was to clarify whether STL is related to sperm quality and its clinical outcomes. For that, we evaluated the STL of patients undergoing infertility treatments and analyzed its relationship with all the seminal parameters, chromatin maturity, sDNAfrag, aneuploidies and oxidative profile, as well as the clinical parameters after the treatments, using swim-up purified spermatozoa. No statistically significant differences were observed in relation to the seminal parameters in the population of purified spermatozoa, suggesting the importance of this method of selection for clinical use. Another original aspect was the observation that the population with the highest STL is reduced, but it has a higher rate of pregnancy (40%), and that the majority of the population is located in an STL range in which the rate of pregnancy is lower (29 %). In future studies we intend to increase the sample size to consolidate the obtained results and to complement them with additional methods. |
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