Publicação
Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure
| Resumo: | High Production Volume Chemicals (HPVCs) are contaminants that pose serious threats to aquatic environments and species that inhabit them, given their massive production and ubiquitous distribution across biological compartments. Among them, organophosphate esters (OPEs) are of particular concern, as they are widely used as plasticizers and flame-retardants, and linked to various forms of toxicity in marine organisms. In this study, we investigated the ecotoxicological response of juvenile gilthead seabream Sparus aurata to the OPE tris (2-chloroisopropyl) phosphate (TCPP) following chronic dietary exposure to three different concentrations (low, D1: 0.2 mg kg−1; ecologically relevant, D2: 2 mg kg−1; and high, D3: 10 mg kg−1). Different biomarkers indicative of antioxidant defence mechanisms (catalase, CAT, glutathione S-transferase, GST, activities), metabolism (citrate synthase, CS, lactate dehydrogenase, LDH, activities) and endocrine disruption (vitellogenin content, VTG), as well as cell (lipid peroxidation levels, LPO) and protein damage (ubiquitin content, UBI) were analyzed in liver and muscle to assess TCPP toxicity. High concentrations of TCPP affected S. aurata growth, but not overall fitness condition. Furthermore, metabolic disruption and severe oxidative damages were observed, regardless of exposure dose. VTG content significantly decreased after exposure to all TCPP dosages, indicating a possible masculinization effect. These findings provide new insights to the scientific knowledge on TCPP ecotoxicological attributes and impacts on marine ichthyofauna. In addition, our results confirm the relevance of conducting integrated multi-biomarker approaches to disclose the ecotoxicological effects of poorly studied chemical contaminants and, ultimately, implement wastewater treatment strategies and legislation to protect marine ecosystems from pollution. |
|---|---|
| Autores principais: | Gomes, Rita V. C. |
| Outros Autores: | Borrull, Sílvia; Pereira, Alícia; Dias, Marta; Cereja, Rui; Barata, Marisa; Pousão-Ferreira, Pedro; Faria, Ana M.; Pocurull, Eva; Marcé, Rosa Maria; Marques, António; Maulvault, Ana Luísa |
| Assunto: | Chronic exposure Ecotoxicity Emerging contaminants High production volume chemicals Organophosphate esters Oceanography Aquatic Science Pollution SDG 14 - Life Below Water |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868982887344242688 |
|---|---|
| author | Gomes, Rita V. C. |
| author2 | Borrull, Sílvia Pereira, Alícia Dias, Marta Cereja, Rui Barata, Marisa Pousão-Ferreira, Pedro Faria, Ana M. Pocurull, Eva Marcé, Rosa Maria Marques, António Maulvault, Ana Luísa |
| author2_role | author author author author author author author author author author author |
| author_facet | Gomes, Rita V. C. Borrull, Sílvia Pereira, Alícia Dias, Marta Cereja, Rui Barata, Marisa Pousão-Ferreira, Pedro Faria, Ana M. Pocurull, Eva Marcé, Rosa Maria Marques, António Maulvault, Ana Luísa |
| author_role | author |
| contributor_name_str_mv | UCIBIO - Applied Molecular Biosciences Unit Elsevier RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Gomes, Rita V. C.\"},{\"Person.name\":\"Borrull, Sílvia\"},{\"Person.name\":\"Pereira, Alícia\"},{\"Person.name\":\"Dias, Marta\"},{\"Person.name\":\"Cereja, Rui\"},{\"Person.name\":\"Barata, Marisa\"},{\"Person.name\":\"Pousão-Ferreira, Pedro\"},{\"Person.name\":\"Faria, Ana M.\"},{\"Person.name\":\"Pocurull, Eva\"},{\"Person.name\":\"Marcé, Rosa Maria\"},{\"Person.name\":\"Marques, António\"},{\"Person.name\":\"Maulvault, Ana Luísa\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | UCIBIO - Applied Molecular Biosciences Unit Elsevier RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Gomes, Rita V. C. Borrull, Sílvia Pereira, Alícia Dias, Marta Cereja, Rui Barata, Marisa Pousão-Ferreira, Pedro Faria, Ana M. Pocurull, Eva Marcé, Rosa Maria Marques, António Maulvault, Ana Luísa |
| datacite.date.Accepted.fl_str_mv | 2025-04-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-06-24T21:19:00Z |
| datacite.date.embargoed.fl_str_mv | 2025-06-24T21:19:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Chronic exposure Ecotoxicity Emerging contaminants High production volume chemicals Organophosphate esters Oceanography Aquatic Science Pollution SDG 14 - Life Below Water |
| datacite.titles.title.fl_str_mv | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure Juvenile gilthead seabream (Sparus aurata) as a case study |
| dc.contributor.none.fl_str_mv | UCIBIO - Applied Molecular Biosciences Unit Elsevier RUN |
| dc.creator.none.fl_str_mv | Gomes, Rita V. C. Borrull, Sílvia Pereira, Alícia Dias, Marta Cereja, Rui Barata, Marisa Pousão-Ferreira, Pedro Faria, Ana M. Pocurull, Eva Marcé, Rosa Maria Marques, António Maulvault, Ana Luísa |
| dc.date.Accepted.fl_str_mv | 2025-04-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-06-24T21:19:00Z |
| dc.date.embargoed.fl_str_mv | 2025-06-24T21:19:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/184407 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Chronic exposure Ecotoxicity Emerging contaminants High production volume chemicals Organophosphate esters Oceanography Aquatic Science Pollution SDG 14 - Life Below Water |
| dc.title.fl_str_mv | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure Juvenile gilthead seabream (Sparus aurata) as a case study |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | High Production Volume Chemicals (HPVCs) are contaminants that pose serious threats to aquatic environments and species that inhabit them, given their massive production and ubiquitous distribution across biological compartments. Among them, organophosphate esters (OPEs) are of particular concern, as they are widely used as plasticizers and flame-retardants, and linked to various forms of toxicity in marine organisms. In this study, we investigated the ecotoxicological response of juvenile gilthead seabream Sparus aurata to the OPE tris (2-chloroisopropyl) phosphate (TCPP) following chronic dietary exposure to three different concentrations (low, D1: 0.2 mg kg−1; ecologically relevant, D2: 2 mg kg−1; and high, D3: 10 mg kg−1). Different biomarkers indicative of antioxidant defence mechanisms (catalase, CAT, glutathione S-transferase, GST, activities), metabolism (citrate synthase, CS, lactate dehydrogenase, LDH, activities) and endocrine disruption (vitellogenin content, VTG), as well as cell (lipid peroxidation levels, LPO) and protein damage (ubiquitin content, UBI) were analyzed in liver and muscle to assess TCPP toxicity. High concentrations of TCPP affected S. aurata growth, but not overall fitness condition. Furthermore, metabolic disruption and severe oxidative damages were observed, regardless of exposure dose. VTG content significantly decreased after exposure to all TCPP dosages, indicating a possible masculinization effect. These findings provide new insights to the scientific knowledge on TCPP ecotoxicological attributes and impacts on marine ichthyofauna. In addition, our results confirm the relevance of conducting integrated multi-biomarker approaches to disclose the ecotoxicological effects of poorly studied chemical contaminants and, ultimately, implement wastewater treatment strategies and legislation to protect marine ecosystems from pollution. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/1595743f-6f57-4497-ab18-1eb38891bbc3/download |
| funder_facet_str_mv | FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 3599-PPCDT |
| id | run_cdd490dddcef73428f05d3cd3e7bbf9c |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/184407 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/184407 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Gomes, Rita V. C. Borrull, Sílvia Pereira, Alícia Dias, Marta Cereja, Rui Barata, Marisa Pousão-Ferreira, Pedro Faria, Ana M. Pocurull, Eva Marcé, Rosa Maria Marques, António Maulvault, Ana Luísa |
| publishDate | 2025 |
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| reponame_str | Repositório Institucional da UNL |
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| spelling | engenHigh Production Volume Chemicals (HPVCs) are contaminants that pose serious threats to aquatic environments and species that inhabit them, given their massive production and ubiquitous distribution across biological compartments. Among them, organophosphate esters (OPEs) are of particular concern, as they are widely used as plasticizers and flame-retardants, and linked to various forms of toxicity in marine organisms. In this study, we investigated the ecotoxicological response of juvenile gilthead seabream Sparus aurata to the OPE tris (2-chloroisopropyl) phosphate (TCPP) following chronic dietary exposure to three different concentrations (low, D1: 0.2 mg kg−1; ecologically relevant, D2: 2 mg kg−1; and high, D3: 10 mg kg−1). Different biomarkers indicative of antioxidant defence mechanisms (catalase, CAT, glutathione S-transferase, GST, activities), metabolism (citrate synthase, CS, lactate dehydrogenase, LDH, activities) and endocrine disruption (vitellogenin content, VTG), as well as cell (lipid peroxidation levels, LPO) and protein damage (ubiquitin content, UBI) were analyzed in liver and muscle to assess TCPP toxicity. High concentrations of TCPP affected S. aurata growth, but not overall fitness condition. Furthermore, metabolic disruption and severe oxidative damages were observed, regardless of exposure dose. VTG content significantly decreased after exposure to all TCPP dosages, indicating a possible masculinization effect. These findings provide new insights to the scientific knowledge on TCPP ecotoxicological attributes and impacts on marine ichthyofauna. In addition, our results confirm the relevance of conducting integrated multi-biomarker approaches to disclose the ecotoxicological effects of poorly studied chemical contaminants and, ultimately, implement wastewater treatment strategies and legislation to protect marine ecosystems from pollution.application/pdfenEcotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposureSubtitleenJuvenile gilthead seabream (Sparus aurata) as a case studyGomes, Rita V. C.Borrull, SílviaPereira, AlíciaDias, MartaCereja, RuiBarata, MarisaPousão-Ferreira, PedroFaria, Ana M.Pocurull, EvaMarcé, Rosa MariaMarques, AntónioMaulvault, Ana LuísaUCIBIO - Applied Molecular Biosciences UnitElsevierHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf0025-326XURNIsPartOfPURE: 119243994URNIsPartOfPURE UUID: 735746f8-c8d4-4052-bc51-5c0bef1042fbURNIsPartOfScopus: 85216862256URNIsPartOfPubMed: 39914116URNIsPartOfWOS: 001423521400001URNIsPartOfPubMed: 39914116DOIIsPartOf10.1016/j.marpolbul.2025.1176282025-06-24T21:19:00Z2025-042025-04-01T00:00:00ZHandlehttp://hdl.handle.net/10362/184407http://purl.org/coar/access_right/c_abf2open accessChronic exposureEcotoxicityEmerging contaminantsHigh production volume chemicalsOrganophosphate estersOceanographyAquatic SciencePollutionSDG 14 - Life Below Water2406198 bytesFundação para a Ciência e a TecnologiaSoluções eco-inovadoras para adaptação da aquacultura aos desafios das alterações climáticas: uma abordagem multi-ómica integrada para avaliar as respostas imuno-metabólicas dos peixes marinhos3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/1595743f-6f57-4497-ab18-1eb38891bbc3/download |
| spellingShingle | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure Gomes, Rita V. C. Chronic exposure Ecotoxicity Emerging contaminants High production volume chemicals Organophosphate esters Oceanography Aquatic Science Pollution SDG 14 - Life Below Water |
| status | SINGLETON |
| subject.fl_str_mv | Chronic exposure Ecotoxicity Emerging contaminants High production volume chemicals Organophosphate esters Oceanography Aquatic Science Pollution SDG 14 - Life Below Water |
| title | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure |
| title_full | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure |
| title_fullStr | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure |
| title_full_unstemmed | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure |
| title_short | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure |
| title_sort | Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure |
| topic | Chronic exposure Ecotoxicity Emerging contaminants High production volume chemicals Organophosphate esters Oceanography Aquatic Science Pollution SDG 14 - Life Below Water |
| topic_facet | Chronic exposure Ecotoxicity Emerging contaminants High production volume chemicals Organophosphate esters Oceanography Aquatic Science Pollution SDG 14 - Life Below Water |
| url | http://hdl.handle.net/10362/184407 |
| visible | 1 |