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Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions

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Resumo:Information on localization of Al and As in Tamarix gallica is required in order to better understand the detoxification mechanisms that confer tolerance in this halophyte plant species. Plants were subjected to different Al and As concentrations with and without salt supplementation. High concentrations of As and Al have been found in Tamarix gallica leaves and roots without symptoms of toxicity to the plant, which may be related to the particular compartmentation. A sequential extraction was carried out on leaves and roots to determine and to compare the metal compartmentation in the plant. In this study, subcellular localization of As and Al was determined for the first time in roots and leaves of T. gallica, and provided evidence of the detoxification mechanisms of high As and Al concentrations. These results suggest that the subcellular distribution of As and Al play important roles in avoidance of metal toxicity. The most part of Al (that has high toxicity to the plant when available forms are present) was immobilized in cell wall, potentially suppressing its transportation to other subcellular compartments more susceptible to Al toxicity. On the other hand, the greater sequestration of As in the vacuole reduces its toxicity to the remaining cell organelles in the roots, but cell wall confinement remains an important tolerant mechanism in the leaves.
Autores principais:Sghaier, Dhouha Belhaj
Outros Autores:Pedro, Sílvia; Diniz, Mário S.; Duarte, Bernardo; Caçador, Isabel; Sleimi, Noomene
Assunto:Aluminum Arsenic Cellular compartmentation Detoxification Tamarix gallica Oceanography Global and Planetary Change Water Science and Technology Ocean Engineering Aquatic Science
Ano:2016
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade Nova de Lisboa
Idioma:inglês
Origem:Repositório Institucional da UNL
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author Sghaier, Dhouha Belhaj
author2 Pedro, Sílvia
Diniz, Mário S.
Duarte, Bernardo
Caçador, Isabel
Sleimi, Noomene
author2_role author
author
author
author
author
author_facet Sghaier, Dhouha Belhaj
Pedro, Sílvia
Diniz, Mário S.
Duarte, Bernardo
Caçador, Isabel
Sleimi, Noomene
author_role author
contributor_name_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
Frontiers Media
RUN
country_str PT
creators_json_txt [{\"Person.name\":\"Sghaier, Dhouha Belhaj\"},{\"Person.name\":\"Pedro, Sílvia\"},{\"Person.name\":\"Diniz, Mário S.\"},{\"Person.name\":\"Duarte, Bernardo\"},{\"Person.name\":\"Caçador, Isabel\"},{\"Person.name\":\"Sleimi, Noomene\"}]
datacite.contributors.contributor.contributorName.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
Frontiers Media
RUN
datacite.creators.creator.creatorName.fl_str_mv Sghaier, Dhouha Belhaj
Pedro, Sílvia
Diniz, Mário S.
Duarte, Bernardo
Caçador, Isabel
Sleimi, Noomene
datacite.date.Accepted.fl_str_mv 2016-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2018-05-09T22:10:51Z
datacite.date.embargoed.fl_str_mv 2018-05-09T22:10:51Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Aluminum
Arsenic
Cellular compartmentation
Detoxification
Tamarix gallica
Oceanography
Global and Planetary Change
Water Science and Technology
Ocean Engineering
Aquatic Science
datacite.titles.title.fl_str_mv Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
Frontiers Media
RUN
dc.creator.none.fl_str_mv Sghaier, Dhouha Belhaj
Pedro, Sílvia
Diniz, Mário S.
Duarte, Bernardo
Caçador, Isabel
Sleimi, Noomene
dc.date.Accepted.fl_str_mv 2016-01-01T00:00:00Z
dc.date.available.fl_str_mv 2018-05-09T22:10:51Z
dc.date.embargoed.fl_str_mv 2018-05-09T22:10:51Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://www.scopus.com/inward/record.url?scp=85008716265&partnerID=8YFLogxK
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Aluminum
Arsenic
Cellular compartmentation
Detoxification
Tamarix gallica
Oceanography
Global and Planetary Change
Water Science and Technology
Ocean Engineering
Aquatic Science
dc.title.fl_str_mv Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Information on localization of Al and As in Tamarix gallica is required in order to better understand the detoxification mechanisms that confer tolerance in this halophyte plant species. Plants were subjected to different Al and As concentrations with and without salt supplementation. High concentrations of As and Al have been found in Tamarix gallica leaves and roots without symptoms of toxicity to the plant, which may be related to the particular compartmentation. A sequential extraction was carried out on leaves and roots to determine and to compare the metal compartmentation in the plant. In this study, subcellular localization of As and Al was determined for the first time in roots and leaves of T. gallica, and provided evidence of the detoxification mechanisms of high As and Al concentrations. These results suggest that the subcellular distribution of As and Al play important roles in avoidance of metal toxicity. The most part of Al (that has high toxicity to the plant when available forms are present) was immobilized in cell wall, potentially suppressing its transportation to other subcellular compartments more susceptible to Al toxicity. On the other hand, the greater sequestration of As in the vacuole reduces its toxicity to the remaining cell organelles in the roots, but cell wall confinement remains an important tolerant mechanism in the leaves.
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eu_rights_str_mv openAccess
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inst_facet_str urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa
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institution Universidade Nova de Lisboa
instname_str Universidade Nova de Lisboa
language eng
network_acronym_str run
network_name_str Repositório Institucional da UNL
oai_identifier_str oai:run.unl.pt:10362/36326
organization_str_mv urn:organizationAcronym:unl
person_str_mv Sghaier, Dhouha Belhaj
Pedro, Sílvia
Diniz, Mário S.
Duarte, Bernardo
Caçador, Isabel
Sleimi, Noomene
publishDate 2016
repo_facet_str urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL
reponame_str Repositório Institucional da UNL
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spelling engenInformation on localization of Al and As in Tamarix gallica is required in order to better understand the detoxification mechanisms that confer tolerance in this halophyte plant species. Plants were subjected to different Al and As concentrations with and without salt supplementation. High concentrations of As and Al have been found in Tamarix gallica leaves and roots without symptoms of toxicity to the plant, which may be related to the particular compartmentation. A sequential extraction was carried out on leaves and roots to determine and to compare the metal compartmentation in the plant. In this study, subcellular localization of As and Al was determined for the first time in roots and leaves of T. gallica, and provided evidence of the detoxification mechanisms of high As and Al concentrations. These results suggest that the subcellular distribution of As and Al play important roles in avoidance of metal toxicity. The most part of Al (that has high toxicity to the plant when available forms are present) was immobilized in cell wall, potentially suppressing its transportation to other subcellular compartments more susceptible to Al toxicity. On the other hand, the greater sequestration of As in the vacuole reduces its toxicity to the remaining cell organelles in the roots, but cell wall confinement remains an important tolerant mechanism in the leaves.application/pdfenTissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditionsSghaier, Dhouha BelhajPedro, SílviaDiniz, Mário S.Duarte, BernardoCaçador, IsabelSleimi, NoomeneDQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitFrontiers MediaHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2296-7745URNIsPartOfPURE: 2506449URNIsPartOfPURE UUID: add16ae3-e405-4026-affa-37b52a076967URNIsPartOfScopus: 85008716265DOIIsPartOf10.3389/fmars.2016.002742018-05-09T22:10:51Z20162016-01-01T00:00:00ZURLhttp://www.scopus.com/inward/record.url?scp=85008716265&partnerID=8YFLogxKhttp://purl.org/coar/access_right/c_abf2open accessAluminumArsenicCellular compartmentationDetoxificationTamarix gallicaOceanographyGlobal and Planetary ChangeWater Science and TechnologyOcean EngineeringAquatic Science1814537 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/04cdfea4-bbcb-4b6f-a806-e970c8db12f7/download
spellingShingle Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
Sghaier, Dhouha Belhaj
Aluminum
Arsenic
Cellular compartmentation
Detoxification
Tamarix gallica
Oceanography
Global and Planetary Change
Water Science and Technology
Ocean Engineering
Aquatic Science
status SINGLETON
subject.fl_str_mv Aluminum
Arsenic
Cellular compartmentation
Detoxification
Tamarix gallica
Oceanography
Global and Planetary Change
Water Science and Technology
Ocean Engineering
Aquatic Science
title Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
title_full Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
title_fullStr Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
title_full_unstemmed Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
title_short Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
title_sort Tissue localization and distribution of as and al in the halophyte Tamarix gallica under controlled conditions
topic Aluminum
Arsenic
Cellular compartmentation
Detoxification
Tamarix gallica
Oceanography
Global and Planetary Change
Water Science and Technology
Ocean Engineering
Aquatic Science
topic_facet Aluminum
Arsenic
Cellular compartmentation
Detoxification
Tamarix gallica
Oceanography
Global and Planetary Change
Water Science and Technology
Ocean Engineering
Aquatic Science
url http://www.scopus.com/inward/record.url?scp=85008716265&partnerID=8YFLogxK
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