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Multi-Omic approaches to unravel phenotypic responses of the tropical fish Lutjanus guttatus to ocean warming and acidification scenarios

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Resumo:Anthropogenic CO2 emissions are contributing to rising seawater temperatures and ocean acidification, posing significant challenges for marine fish, which must either adapt physio- logically or migrate to avoid deteriorating environmental conditions. This study aimed to in- vestigate the molecular networks and biological pathways underlying the response of the spotted rose snapper ( Lutjanus guttatus) to a mid-century scenario of ocean warming and acidification. To achieve this, quantitative metabolomics and proteomics were employed to analyze the livers of fish exposed for 28 days to two fully crossed experimental treatments (28 ºC – control, 30 ºC – warming) and pCO2 (400 ppm – control, 700 ppm – acidification). Out of 45 identified primary metabolites, only glucose was significantly downregulated, while pro- teomics analysis identified 27 differentially regulated proteins (DRPs) across all contrasts. Pathway analysis revealed no significantly enriched pathways at the metabolite level, with retinol metabolism emerging as the only enriched pathway at the protein level. Ocean acidifi- cation during current temperatures did not affect protein regulation. However, when in- creased pCO2 levels coincided with elevated temperatures, lipid metabolism was altered and retinol metabolism showed increased activity. In response to elevated temperature L. guttatus exhibited a clear molecular stress response, characterized by the upregulation of HSP90 re- gardless of pCO2 treatment. Glucose concentrations decreased when increasing temperatures ambient pCO2, which is the opposite reaction of what has been reported in literature. This suggests that L. guttatus may exhibit a unique metabolic response to warming. Under the most extreme conditions, glucose levels returned to control values. The combined stress of warming and acidification led to increased cellular repair investment, demonstrating the intricate inter- play between temperature and pCO2 on fish liver physiology. These findings suggest that L. guttatus is capable of responding to climate stressors through non-standard metabolic adap- tations.
Autores principais:Lohmann, David Jeremy
Assunto:fish physiology multi-omics ocean warming acidification climate change
Ano:2024
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso aberto
Instituição associada:Universidade Nova de Lisboa
Idioma:inglês
Origem:Repositório Institucional da UNL

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