Publicação
Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water
| Resumo: | The presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus species survive in the natural environment due to their tolerance to a wide range of temperatures, dryness, dehydration, and low water activity. Therefore, we aimed to evaluate the influence of external environmental factors on the formation of biofilm of staphylococci isolated from hospital wastewater and surface waters. We investigated the biofilm formation of methicillin-resistant and -susceptible S. aureus (MRSA and MSSA) and coagulase-negative staphylococci (CoNS) under various temperatures, pH values, salt concentrations, glucose concentrations, and under anaerobic and aerobic conditions. CoNS had the ability to produce more biofilm biomass than MSSA and MRSA. All environmental factors studied influenced the biofilm formation of staphylococci isolates after 24 h of incubation. Higher biofilm formation was achieved at 4% of NaCl and 0.5% of glucose for MSSA and CoNS, and 1% of NaCl and 1.5% of glucose for MRSA isolates. Biofilm formation of isolates was greater at 25 °C and 37 °C than at 10 °C and 4 °C. pH values between 6 and 8 led to more robust biofilm formation than pH levels of 9 and 5. Although staphylococci are facultative anaerobes, biofilm formation was higher in the presence of oxygen. The results demonstrated that multiple environmental factors affect staphylococci biofilm formation. Different conditions affect differently the biofilm formation of MRSA, MSSA, and CoNS strains. |
|---|---|
| Autores principais: | Silva, Vanessa |
| Outros Autores: | Pereira, José Eduardo; Maltez, Luís; Poeta, Patrícia; Igrejas, Gilberto |
| Assunto: | biofilms coagulase-negative staphylococci environment S. aureus Staphylococcus Immunology and Allergy Molecular Biology General Immunology and Microbiology Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983015106936832 |
|---|---|
| author | Silva, Vanessa |
| author2 | Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto |
| author2_role | author author author author |
| author_facet | Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto |
| author_role | author |
| contributor_name_str_mv | LAQV@REQUIMTE DQ - Departamento de Química MDPI - Multidisciplinary Digital Publishing Institute RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silva, Vanessa\"},{\"Person.name\":\"Pereira, José Eduardo\"},{\"Person.name\":\"Maltez, Luís\"},{\"Person.name\":\"Poeta, Patrícia\"},{\"Person.name\":\"Igrejas, Gilberto\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | LAQV@REQUIMTE DQ - Departamento de Química MDPI - Multidisciplinary Digital Publishing Institute RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto |
| datacite.date.Accepted.fl_str_mv | 2022-09-20T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-15T22:35:08Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-15T22:35:08Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | biofilms coagulase-negative staphylococci environment S. aureus Staphylococcus Immunology and Allergy Molecular Biology General Immunology and Microbiology Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| dc.contributor.none.fl_str_mv | LAQV@REQUIMTE DQ - Departamento de Química MDPI - Multidisciplinary Digital Publishing Institute RUN |
| dc.creator.none.fl_str_mv | Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto |
| dc.date.Accepted.fl_str_mv | 2022-09-20T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-15T22:35:08Z |
| dc.date.embargoed.fl_str_mv | 2023-03-15T22:35:08Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/150650 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | biofilms coagulase-negative staphylococci environment S. aureus Staphylococcus Immunology and Allergy Molecular Biology General Immunology and Microbiology Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus species survive in the natural environment due to their tolerance to a wide range of temperatures, dryness, dehydration, and low water activity. Therefore, we aimed to evaluate the influence of external environmental factors on the formation of biofilm of staphylococci isolated from hospital wastewater and surface waters. We investigated the biofilm formation of methicillin-resistant and -susceptible S. aureus (MRSA and MSSA) and coagulase-negative staphylococci (CoNS) under various temperatures, pH values, salt concentrations, glucose concentrations, and under anaerobic and aerobic conditions. CoNS had the ability to produce more biofilm biomass than MSSA and MRSA. All environmental factors studied influenced the biofilm formation of staphylococci isolates after 24 h of incubation. Higher biofilm formation was achieved at 4% of NaCl and 0.5% of glucose for MSSA and CoNS, and 1% of NaCl and 1.5% of glucose for MRSA isolates. Biofilm formation of isolates was greater at 25 °C and 37 °C than at 10 °C and 4 °C. pH values between 6 and 8 led to more robust biofilm formation than pH levels of 9 and 5. Although staphylococci are facultative anaerobes, biofilm formation was higher in the presence of oxygen. The results demonstrated that multiple environmental factors affect staphylococci biofilm formation. Different conditions affect differently the biofilm formation of MRSA, MSSA, and CoNS strains. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/0d2f74a3-c2e3-4d57-8a9b-968cd4aae26d/download |
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| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID POR_NORTE |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10362/150650 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
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| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
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| oai_identifier_str | oai:run.unl.pt:10362/150650 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Silva, Vanessa Pereira, José Eduardo Maltez, Luís Poeta, Patrícia Igrejas, Gilberto |
| publishDate | 2022 |
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| spelling | engenThe presence of biofilms can negatively affect several different areas, such as the food industry, environment, and biomedical sectors. Conditions under which bacteria grow and develop, such as temperature, nutrients, and pH, among others, can largely influence biofilm production. Staphylococcus species survive in the natural environment due to their tolerance to a wide range of temperatures, dryness, dehydration, and low water activity. Therefore, we aimed to evaluate the influence of external environmental factors on the formation of biofilm of staphylococci isolated from hospital wastewater and surface waters. We investigated the biofilm formation of methicillin-resistant and -susceptible S. aureus (MRSA and MSSA) and coagulase-negative staphylococci (CoNS) under various temperatures, pH values, salt concentrations, glucose concentrations, and under anaerobic and aerobic conditions. CoNS had the ability to produce more biofilm biomass than MSSA and MRSA. All environmental factors studied influenced the biofilm formation of staphylococci isolates after 24 h of incubation. Higher biofilm formation was achieved at 4% of NaCl and 0.5% of glucose for MSSA and CoNS, and 1% of NaCl and 1.5% of glucose for MRSA isolates. Biofilm formation of isolates was greater at 25 °C and 37 °C than at 10 °C and 4 °C. pH values between 6 and 8 led to more robust biofilm formation than pH levels of 9 and 5. Although staphylococci are facultative anaerobes, biofilm formation was higher in the presence of oxygen. The results demonstrated that multiple environmental factors affect staphylococci biofilm formation. Different conditions affect differently the biofilm formation of MRSA, MSSA, and CoNS strains.application/pdfenInfluence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface WaterSilva, VanessaPereira, José EduardoMaltez, LuísPoeta, PatríciaIgrejas, GilbertoLAQV@REQUIMTEDQ - Departamento de QuímicaMDPI - Multidisciplinary Digital Publishing InstituteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2076-0817URNIsPartOfPURE: 55918605URNIsPartOfPURE UUID: 4ba5fd4b-2ea1-450a-b94d-d9e9b3b411abURNIsPartOfScopus: 85140831719URNIsPartOfWOS: 000873859700001URNIsPartOfPubMed: 36297126URNIsPartOfPubMedCentral: PMC9611571DOIIsPartOf10.3390/pathogens111010692023-03-15T22:35:08Z2022-09-202022-09-20T00:00:00ZHandlehttp://hdl.handle.net/10362/150650http://purl.org/coar/access_right/c_abf2open accessbiofilmscoagulase-negative staphylococcienvironmentS. aureusStaphylococcusImmunology and AllergyMolecular BiologyGeneral Immunology and MicrobiologyMicrobiology (medical)Infectious DiseasesSDG 3 - Good Health and Well-being2307508 bytesFundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processes6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processes6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a Tecnologiaalterado para: Antimicrobial resistance, clonal diversity and biofilm formation of Staphylococcus aureus and other staphylococci: A One Health approach to a global threat Integrating multiple ‘omics’ analysis of methicillin-resistant Staphylococcus aureus MRSA biofilms: a One Health approach to a global threatPOR_NORTECrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/0d2f74a3-c2e3-4d57-8a9b-968cd4aae26d/download |
| spellingShingle | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water Silva, Vanessa biofilms coagulase-negative staphylococci environment S. aureus Staphylococcus Immunology and Allergy Molecular Biology General Immunology and Microbiology Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | biofilms coagulase-negative staphylococci environment S. aureus Staphylococcus Immunology and Allergy Molecular Biology General Immunology and Microbiology Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
| title | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| title_full | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| title_fullStr | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| title_full_unstemmed | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| title_short | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| title_sort | Influence of Environmental Factors on Biofilm Formation of Staphylococci Isolated from Wastewater and Surface Water |
| topic | biofilms coagulase-negative staphylococci environment S. aureus Staphylococcus Immunology and Allergy Molecular Biology General Immunology and Microbiology Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
| topic_facet | biofilms coagulase-negative staphylococci environment S. aureus Staphylococcus Immunology and Allergy Molecular Biology General Immunology and Microbiology Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/150650 |
| visible | 1 |