Publicação
Poultry farms as reservoirs of azole-resistant fungi
| Resumo: | The intensification of poultry farming has raises concerns regarding microbial contamination and antifungal resistance, particularly involving Aspergillus fumigatus, a key pathogen presenting clinical relevance. This study aims to present the first in-depth evaluation of environmental conditions, airborne particles, microbial contamination, and fungal resistance to azole drugs in poultry farm environments. A multi-approach sampling strategy (passive and active sampling methods) was conducted throughout the poultry production cycle. Microbial characterization was performed, combining culture-dependent methods and molecular techniques with a focus on fungal diversity and azole resistance. Bacteria and fungal loads indoors exceed the outdoor levels in 80.85 % (n = 38/47) and 78.72 % (n = 37/47) of air samples, respectively. Toxigenic fungal species (Aspergillus spp. and Penicilium spp.) were widespread (air, swabs, electrostatic dust cloths,feed, bed). Fungi resistant to at least one antifungal (itraconazole, voriconazole, posaconazole) was found in 53.3 % of the total samples collected inside poultry pavilions (n = 45/126), including potentially azole-resistant Aspergillus species. Particulate matter acts as a carrier of microorganisms, enhancing workers respiratory exposure risks. Electrostatic dust cloths proved to be a valuable sampling method for exposure assessment to potential pathogenic and resistant fungi. Our findings identify poultry farms as potential hotspots for toxigenic and azole-resistant fungi with implications for occupational health. |
|---|---|
| Autores principais: | Gomes, Bianca |
| Outros Autores: | Dias, Marta; Cervantes, Renata; Pena, Pedro; Carolino, Elisabete; Caetano, Liliana Aranha; Viegas, Susana; Viegas, Carla |
| Assunto: | Aspergillus spp. Azole resistance Occupational exposure Particulate matter Poultry farms Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868415438461861888 |
|---|---|
| author | Gomes, Bianca |
| author2 | Dias, Marta Cervantes, Renata Pena, Pedro Carolino, Elisabete Caetano, Liliana Aranha Viegas, Susana Viegas, Carla |
| author2_role | author author author author author author author |
| author_facet | Gomes, Bianca Dias, Marta Cervantes, Renata Pena, Pedro Carolino, Elisabete Caetano, Liliana Aranha Viegas, Susana Viegas, Carla |
| author_role | author |
| contributor_name_str_mv | Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP) Centro de Investigação em Saúde Pública (CISP/PHRC) Comprehensive Health Research Centre (CHRC) - Pólo ENSP Escola Nacional de Saúde Pública (ENSP) Elsevier BV RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Gomes, Bianca\"},{\"Person.name\":\"Dias, Marta\"},{\"Person.name\":\"Cervantes, Renata\"},{\"Person.name\":\"Pena, Pedro\"},{\"Person.name\":\"Carolino, Elisabete\"},{\"Person.name\":\"Caetano, Liliana Aranha\"},{\"Person.name\":\"Viegas, Susana\"},{\"Person.name\":\"Viegas, Carla\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP) Centro de Investigação em Saúde Pública (CISP/PHRC) Comprehensive Health Research Centre (CHRC) - Pólo ENSP Escola Nacional de Saúde Pública (ENSP) Elsevier BV RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Gomes, Bianca Dias, Marta Cervantes, Renata Pena, Pedro Carolino, Elisabete Caetano, Liliana Aranha Viegas, Susana Viegas, Carla |
| datacite.date.Accepted.fl_str_mv | 2025-12-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-01-14T15:54:40Z |
| datacite.date.embargoed.fl_str_mv | 2026-01-14T15:54:40Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Aspergillus spp. Azole resistance Occupational exposure Particulate matter Poultry farms Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Poultry farms as reservoirs of azole-resistant fungi occupational health risks in agricultural facilities |
| dc.contributor.none.fl_str_mv | Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP) Centro de Investigação em Saúde Pública (CISP/PHRC) Comprehensive Health Research Centre (CHRC) - Pólo ENSP Escola Nacional de Saúde Pública (ENSP) Elsevier BV RUN |
| dc.creator.none.fl_str_mv | Gomes, Bianca Dias, Marta Cervantes, Renata Pena, Pedro Carolino, Elisabete Caetano, Liliana Aranha Viegas, Susana Viegas, Carla |
| dc.date.Accepted.fl_str_mv | 2025-12-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-01-14T15:54:40Z |
| dc.date.embargoed.fl_str_mv | 2026-01-14T15:54:40Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/199048 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Aspergillus spp. Azole resistance Occupational exposure Particulate matter Poultry farms Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Poultry farms as reservoirs of azole-resistant fungi occupational health risks in agricultural facilities |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The intensification of poultry farming has raises concerns regarding microbial contamination and antifungal resistance, particularly involving Aspergillus fumigatus, a key pathogen presenting clinical relevance. This study aims to present the first in-depth evaluation of environmental conditions, airborne particles, microbial contamination, and fungal resistance to azole drugs in poultry farm environments. A multi-approach sampling strategy (passive and active sampling methods) was conducted throughout the poultry production cycle. Microbial characterization was performed, combining culture-dependent methods and molecular techniques with a focus on fungal diversity and azole resistance. Bacteria and fungal loads indoors exceed the outdoor levels in 80.85 % (n = 38/47) and 78.72 % (n = 37/47) of air samples, respectively. Toxigenic fungal species (Aspergillus spp. and Penicilium spp.) were widespread (air, swabs, electrostatic dust cloths,feed, bed). Fungi resistant to at least one antifungal (itraconazole, voriconazole, posaconazole) was found in 53.3 % of the total samples collected inside poultry pavilions (n = 45/126), including potentially azole-resistant Aspergillus species. Particulate matter acts as a carrier of microorganisms, enhancing workers respiratory exposure risks. Electrostatic dust cloths proved to be a valuable sampling method for exposure assessment to potential pathogenic and resistant fungi. Our findings identify poultry farms as potential hotspots for toxigenic and azole-resistant fungi with implications for occupational health. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/b2ea2cdd-a4fb-4c0e-8925-a4a235295756/download |
| id | run_dc6d3fbd7ad7ceef8a3df1c247a4f3fe |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/199048 |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/199048 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Gomes, Bianca Dias, Marta Cervantes, Renata Pena, Pedro Carolino, Elisabete Caetano, Liliana Aranha Viegas, Susana Viegas, Carla |
| publishDate | 2025 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenThe intensification of poultry farming has raises concerns regarding microbial contamination and antifungal resistance, particularly involving Aspergillus fumigatus, a key pathogen presenting clinical relevance. This study aims to present the first in-depth evaluation of environmental conditions, airborne particles, microbial contamination, and fungal resistance to azole drugs in poultry farm environments. A multi-approach sampling strategy (passive and active sampling methods) was conducted throughout the poultry production cycle. Microbial characterization was performed, combining culture-dependent methods and molecular techniques with a focus on fungal diversity and azole resistance. Bacteria and fungal loads indoors exceed the outdoor levels in 80.85 % (n = 38/47) and 78.72 % (n = 37/47) of air samples, respectively. Toxigenic fungal species (Aspergillus spp. and Penicilium spp.) were widespread (air, swabs, electrostatic dust cloths,feed, bed). Fungi resistant to at least one antifungal (itraconazole, voriconazole, posaconazole) was found in 53.3 % of the total samples collected inside poultry pavilions (n = 45/126), including potentially azole-resistant Aspergillus species. Particulate matter acts as a carrier of microorganisms, enhancing workers respiratory exposure risks. Electrostatic dust cloths proved to be a valuable sampling method for exposure assessment to potential pathogenic and resistant fungi. Our findings identify poultry farms as potential hotspots for toxigenic and azole-resistant fungi with implications for occupational health.application/pdfenPoultry farms as reservoirs of azole-resistant fungiSubtitleenoccupational health risks in agricultural facilitiesGomes, BiancaDias, MartaCervantes, RenataPena, PedroCarolino, ElisabeteCaetano, Liliana AranhaViegas, SusanaViegas, CarlaLaboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP)Centro de Investigação em Saúde Pública (CISP/PHRC)Comprehensive Health Research Centre (CHRC) - Pólo ENSPEscola Nacional de Saúde Pública (ENSP)Elsevier BVHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2352-7714URNIsPartOfPURE: 148907996URNIsPartOfPURE UUID: d2aa6d41-3a90-400f-842b-4d2584390388URNIsPartOfScopus: 105019236639URNIsPartOfPubMed: 41210200URNIsPartOfPubMedCentral: PMC12593707URNIsPartOfWOS: 001606657400001DOIIsPartOf10.1016/j.onehlt.2025.1012302026-01-14T15:54:40Z2025-122025-12-01T00:00:00ZHandlehttp://hdl.handle.net/10362/199048http://purl.org/coar/access_right/c_abf2open accessAspergillus spp.Azole resistanceOccupational exposureParticulate matterPoultry farmsPublic Health, Environmental and Occupational HealthInfectious DiseasesSDG 3 - Good Health and Well-being4327375 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/b2ea2cdd-a4fb-4c0e-8925-a4a235295756/download |
| spellingShingle | Poultry farms as reservoirs of azole-resistant fungi Gomes, Bianca Aspergillus spp. Azole resistance Occupational exposure Particulate matter Poultry farms Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | Aspergillus spp. Azole resistance Occupational exposure Particulate matter Poultry farms Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| title | Poultry farms as reservoirs of azole-resistant fungi |
| title_full | Poultry farms as reservoirs of azole-resistant fungi |
| title_fullStr | Poultry farms as reservoirs of azole-resistant fungi |
| title_full_unstemmed | Poultry farms as reservoirs of azole-resistant fungi |
| title_short | Poultry farms as reservoirs of azole-resistant fungi |
| title_sort | Poultry farms as reservoirs of azole-resistant fungi |
| topic | Aspergillus spp. Azole resistance Occupational exposure Particulate matter Poultry farms Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| topic_facet | Aspergillus spp. Azole resistance Occupational exposure Particulate matter Poultry farms Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/199048 |
| visible | 1 |