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Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour

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Resumo:Background: Maize is a crop in high demand for food purposes and consumers worldwide are increasingly concerned with food quality. However, breeding for improved quality is a complex task and therefore developing tools to select for better quality products is of great importance. Kernel composition, flour pasting behavior, and flour particle size have been previously identified as crucial for maize-based food quality. In this work we carried out a genome-wide association study to identify genomic regions controlling compositional and pasting properties of maize wholemeal flour. Results: A collection of 132 diverse inbred lines, with a considerable representation of the food used Portuguese unique germplasm, was trialed during two seasons, and harvested samples characterized for main compositional traits, flour pasting parameters and mean particle size. The collection was genotyped with the MaizeSNP50 array. SNP-trait associations were tested using a mixed linear model accounting for genetic relatedness. Fifty-seven genomic regions were identified, associated with the 11 different quality-related traits evaluated. Regions controlling multiple traits were detected and potential candidate genes identified. As an example, for two viscosity parameters that reflect the capacity of the starch to absorb water and swell, the strongest common associated region was located near the dull endosperm 1 gene that encodes a starch synthase and is determinant on the starch endosperm structure in maize. Conclusions: This study allowed for identifying relevant regions on the maize genome affecting maize kernel composition and flour pasting behavior, candidate genes for the majority of the quality-associated genomic regions, or the most promising target regions to develop molecular tools to increase efficacy and efficiency of quality traits selection (such as "breadability") within maize breeding programs.
Autores principais:Alves, Mara Lisa
Outros Autores:Carbas, Bruna; Gaspar, Daniel; Paulo, Manuel; Brites, Cláudia; Mendes-Moreira, Pedro; Brites, Carla Moita; Malosetti, Marcos; Van Eeuwijk, Fred; Vaz Patto, Maria Carlota
Assunto:Bread Candidate genes Nutritional quality Pasting behavior Plant breeding Portuguese maize germplasm Zea mays L. Plant Science
Ano:2019
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade Nova de Lisboa
Idioma:inglês
Origem:Repositório Institucional da UNL
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author Alves, Mara Lisa
author2 Carbas, Bruna
Gaspar, Daniel
Paulo, Manuel
Brites, Cláudia
Mendes-Moreira, Pedro
Brites, Carla Moita
Malosetti, Marcos
Van Eeuwijk, Fred
Vaz Patto, Maria Carlota
author2_role author
author
author
author
author
author
author
author
author
author_facet Alves, Mara Lisa
Alves, Mara Lisa
Carbas, Bruna
Gaspar, Daniel
Paulo, Manuel
Brites, Cláudia
Mendes-Moreira, Pedro
Brites, Carla Moita
Malosetti, Marcos
Van Eeuwijk, Fred
Vaz Patto, Maria Carlota
Carbas, Bruna
Gaspar, Daniel
Paulo, Manuel
Brites, Cláudia
Mendes-Moreira, Pedro
Brites, Carla Moita
Malosetti, Marcos
Van Eeuwijk, Fred
Vaz Patto, Maria Carlota
author_role author
contributor_name_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
BioMed Central (BMC)
RUN
country_str PT
creators_json_str [{\"Person.name\":\"Alves, Mara Lisa\"},{\"Person.name\":\"Carbas, Bruna\"},{\"Person.name\":\"Gaspar, Daniel\"},{\"Person.name\":\"Paulo, Manuel\"},{\"Person.name\":\"Brites, Cláudia\"},{\"Person.name\":\"Mendes-Moreira, Pedro\"},{\"Person.name\":\"Brites, Carla Moita\"},{\"Person.name\":\"Malosetti, Marcos\"},{\"Person.name\":\"Van Eeuwijk, Fred\"},{\"Person.name\":\"Vaz Patto, Maria Carlota\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
BioMed Central (BMC)
RUN
datacite.creators.creator.creatorName.fl_str_mv Alves, Mara Lisa
Carbas, Bruna
Gaspar, Daniel
Paulo, Manuel
Brites, Cláudia
Mendes-Moreira, Pedro
Brites, Carla Moita
Malosetti, Marcos
Van Eeuwijk, Fred
Vaz Patto, Maria Carlota
datacite.date.Accepted.fl_str_mv 2019-04-02T00:00:00Z
datacite.date.available.fl_str_mv 2020-04-15T22:37:22Z
datacite.date.embargoed.fl_str_mv 2020-04-15T22:37:22Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Bread
Candidate genes
Nutritional quality
Pasting behavior
Plant breeding
Portuguese maize germplasm
Zea mays L.
Plant Science
datacite.titles.title.fl_str_mv Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
BioMed Central (BMC)
RUN
dc.creator.none.fl_str_mv Alves, Mara Lisa
Carbas, Bruna
Gaspar, Daniel
Paulo, Manuel
Brites, Cláudia
Mendes-Moreira, Pedro
Brites, Carla Moita
Malosetti, Marcos
Van Eeuwijk, Fred
Vaz Patto, Maria Carlota
dc.date.Accepted.fl_str_mv 2019-04-02T00:00:00Z
dc.date.available.fl_str_mv 2020-04-15T22:37:22Z
dc.date.embargoed.fl_str_mv 2020-04-15T22:37:22Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10362/96257
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Bread
Candidate genes
Nutritional quality
Pasting behavior
Plant breeding
Portuguese maize germplasm
Zea mays L.
Plant Science
dc.title.fl_str_mv Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Background: Maize is a crop in high demand for food purposes and consumers worldwide are increasingly concerned with food quality. However, breeding for improved quality is a complex task and therefore developing tools to select for better quality products is of great importance. Kernel composition, flour pasting behavior, and flour particle size have been previously identified as crucial for maize-based food quality. In this work we carried out a genome-wide association study to identify genomic regions controlling compositional and pasting properties of maize wholemeal flour. Results: A collection of 132 diverse inbred lines, with a considerable representation of the food used Portuguese unique germplasm, was trialed during two seasons, and harvested samples characterized for main compositional traits, flour pasting parameters and mean particle size. The collection was genotyped with the MaizeSNP50 array. SNP-trait associations were tested using a mixed linear model accounting for genetic relatedness. Fifty-seven genomic regions were identified, associated with the 11 different quality-related traits evaluated. Regions controlling multiple traits were detected and potential candidate genes identified. As an example, for two viscosity parameters that reflect the capacity of the starch to absorb water and swell, the strongest common associated region was located near the dull endosperm 1 gene that encodes a starch synthase and is determinant on the starch endosperm structure in maize. Conclusions: This study allowed for identifying relevant regions on the maize genome affecting maize kernel composition and flour pasting behavior, candidate genes for the majority of the quality-associated genomic regions, or the most promising target regions to develop molecular tools to increase efficacy and efficiency of quality traits selection (such as "breadability") within maize breeding programs.
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eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://run.unl.pt/bitstreams/9ee8f229-6a46-483c-80f1-3a36e27347f6/download
id run_e31c00a6542e7de0946cd4b986deadc6
identifier.url.fl_str_mv http://hdl.handle.net/10362/96257
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institution Universidade Nova de Lisboa
instname_str Universidade Nova de Lisboa
language eng
network_acronym_str run
network_name_str Repositório Institucional da UNL
oai_identifier_str oai:run.unl.pt:10362/96257
organization_str_mv urn:organizationAcronym:unl
person_str_mv Alves, Mara Lisa
Carbas, Bruna
Gaspar, Daniel
Paulo, Manuel
Brites, Cláudia
Mendes-Moreira, Pedro
Brites, Carla Moita
Malosetti, Marcos
Van Eeuwijk, Fred
Vaz Patto, Maria Carlota
publishDate 2019
reponame_str Repositório Institucional da UNL
repository_id_str urn:repositoryAcronym:run
service_str_mv urn:repositoryAcronym:run
spelling engenBackground: Maize is a crop in high demand for food purposes and consumers worldwide are increasingly concerned with food quality. However, breeding for improved quality is a complex task and therefore developing tools to select for better quality products is of great importance. Kernel composition, flour pasting behavior, and flour particle size have been previously identified as crucial for maize-based food quality. In this work we carried out a genome-wide association study to identify genomic regions controlling compositional and pasting properties of maize wholemeal flour. Results: A collection of 132 diverse inbred lines, with a considerable representation of the food used Portuguese unique germplasm, was trialed during two seasons, and harvested samples characterized for main compositional traits, flour pasting parameters and mean particle size. The collection was genotyped with the MaizeSNP50 array. SNP-trait associations were tested using a mixed linear model accounting for genetic relatedness. Fifty-seven genomic regions were identified, associated with the 11 different quality-related traits evaluated. Regions controlling multiple traits were detected and potential candidate genes identified. As an example, for two viscosity parameters that reflect the capacity of the starch to absorb water and swell, the strongest common associated region was located near the dull endosperm 1 gene that encodes a starch synthase and is determinant on the starch endosperm structure in maize. Conclusions: This study allowed for identifying relevant regions on the maize genome affecting maize kernel composition and flour pasting behavior, candidate genes for the majority of the quality-associated genomic regions, or the most promising target regions to develop molecular tools to increase efficacy and efficiency of quality traits selection (such as "breadability") within maize breeding programs.application/pdfenGenome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flourAlves, Mara LisaCarbas, BrunaGaspar, DanielPaulo, ManuelBrites, CláudiaMendes-Moreira, PedroBrites, Carla MoitaMalosetti, MarcosVan Eeuwijk, FredVaz Patto, Maria CarlotaInstituto de Tecnologia Química e Biológica António Xavier (ITQB)BioMed Central (BMC)HostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1471-2229URNIsPartOfPURE: 17736643URNIsPartOfPURE UUID: 6f903dc5-0dfe-4215-a3c7-b0768e438464URNIsPartOfScopus: 85063803006URNIsPartOfPubMed: 30940081DOIIsPartOf10.1186/s12870-019-1729-72020-04-15T22:37:22Z2019-04-022019-04-02T00:00:00ZHandlehttp://hdl.handle.net/10362/96257http://purl.org/coar/access_right/c_abf2open accessBreadCandidate genesNutritional qualityPasting behaviorPlant breedingPortuguese maize germplasmZea mays L.Plant Science1862680 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/9ee8f229-6a46-483c-80f1-3a36e27347f6/download
spellingShingle Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
Alves, Mara Lisa
Bread
Candidate genes
Nutritional quality
Pasting behavior
Plant breeding
Portuguese maize germplasm
Zea mays L.
Plant Science
Alves, Mara Lisa
Bread
Candidate genes
Nutritional quality
Pasting behavior
Plant breeding
Portuguese maize germplasm
Zea mays L.
Plant Science
status NEW
subject.fl_str_mv Bread
Candidate genes
Nutritional quality
Pasting behavior
Plant breeding
Portuguese maize germplasm
Zea mays L.
Plant Science
title Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
title_full Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
title_fullStr Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
title_full_unstemmed Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
title_short Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
title_sort Genome-wide association study for kernel composition and flour pasting behavior in wholemeal maize flour
topic Bread
Candidate genes
Nutritional quality
Pasting behavior
Plant breeding
Portuguese maize germplasm
Zea mays L.
Plant Science
topic_facet Bread
Candidate genes
Nutritional quality
Pasting behavior
Plant breeding
Portuguese maize germplasm
Zea mays L.
Plant Science
url http://hdl.handle.net/10362/96257
visible 1