Publicação
Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands
| Resumo: | Background: Wolbachia pipientis is an endosymbiont bacterium that induces cytoplasmic incompatibility and inhibits arboviral replication in mosquitoes. This study aimed to assess Wolbachia prevalence and genetic diversity in different mosquito species from Cape Verde. Methods: Mosquitoes were collected on six islands of Cape Verde and identified to species using morphological keys and PCR-based assays. Wolbachia was detected by amplifying a fragment of the surface protein gene (wsp). Multilocus sequence typing (MLST) was performed with five housekeeping genes (coxA, gatB, ftsZ, hcpA, and fbpA) and the wsp hypervariable region (HVR) for strain identification. Identification of wPip groups (wPip-I to wPip-V) was performed using PCR–restriction fragment length polymorphism (RFLP) assay on the ankyrin domain gene pk1. Results: Nine mosquito species were collected, including the major vectors Aedes aegypti, Anopheles arabiensis, Culex pipiens sensu stricto, and Culex quinquefasciatus. Wolbachia was only detected in Cx. pipiens s.s. (100% prevalence), Cx. quinquefasciatus (98.3%), Cx. pipiens/quinquefasciatus hybrids (100%), and Culex tigripes (100%). Based on the results of MLST and wsp hypervariable region typing, Wolbachia from the Cx. pipiens complex was assigned to sequence type 9, wPip clade, and supergroup B. PCR/RFLP analysis revealed three wPip groups in Cape Verde, namely wPip-II, wPip-III, and wPip-IV. wPip-IV was the most prevalent, while wPip-II and wPip-III were found only on Maio and Fogo islands. Wolbachia detected in Cx. tigripes belongs to supergroup B, with no attributed MLST profile, indicating a new strain of Wolbachia in this mosquito species. Conclusions: A high prevalence and diversity of Wolbachia was found in species from the Cx. pipiens complex. This diversity may be related to the mosquito's colonization history on the Cape Verde islands. To the best of our knowledge, this is the first study to detect Wolbachia in Cx. tigripes, which may provide an additional opportunity for biocontrol initiatives. |
|---|---|
| Autores principais: | da Moura, Aires Januário Fernandes |
| Outros Autores: | Valadas, Vera; da Veiga Leal, Silvania; Montalvo Sabino, Eddyson; Sousa, Carla A.; Pinto, João |
| Assunto: | Cape Verde Culex pipiens Culex tigripes Genotyping Mosquitoes Wolbachia RA Public aspects of medicine QR Microbiology Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983162533576704 |
|---|---|
| author | da Moura, Aires Januário Fernandes |
| author2 | Valadas, Vera da Veiga Leal, Silvania Montalvo Sabino, Eddyson Sousa, Carla A. Pinto, João |
| author2_role | author author author author author |
| author_facet | da Moura, Aires Januário Fernandes Valadas, Vera da Veiga Leal, Silvania Montalvo Sabino, Eddyson Sousa, Carla A. Pinto, João |
| author_role | author |
| contributor_name_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) BioMed Central (BMC) RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"da Moura, Aires Januário Fernandes\"},{\"Person.name\":\"Valadas, Vera\"},{\"Person.name\":\"da Veiga Leal, Silvania\"},{\"Person.name\":\"Montalvo Sabino, Eddyson\"},{\"Person.name\":\"Sousa, Carla A.\"},{\"Person.name\":\"Pinto, João\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) BioMed Central (BMC) RUN |
| datacite.creators.creator.creatorName.fl_str_mv | da Moura, Aires Januário Fernandes Valadas, Vera da Veiga Leal, Silvania Montalvo Sabino, Eddyson Sousa, Carla A. Pinto, João |
| datacite.date.Accepted.fl_str_mv | 2023-12-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-06-23T22:24:08Z |
| datacite.date.embargoed.fl_str_mv | 2023-06-23T22:24:08Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cape Verde Culex pipiens Culex tigripes Genotyping Mosquitoes Wolbachia RA Public aspects of medicine QR Microbiology Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| dc.contributor.none.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) BioMed Central (BMC) RUN |
| dc.creator.none.fl_str_mv | da Moura, Aires Januário Fernandes Valadas, Vera da Veiga Leal, Silvania Montalvo Sabino, Eddyson Sousa, Carla A. Pinto, João |
| dc.date.Accepted.fl_str_mv | 2023-12-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-06-23T22:24:08Z |
| dc.date.embargoed.fl_str_mv | 2023-06-23T22:24:08Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/154352 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cape Verde Culex pipiens Culex tigripes Genotyping Mosquitoes Wolbachia RA Public aspects of medicine QR Microbiology Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: Wolbachia pipientis is an endosymbiont bacterium that induces cytoplasmic incompatibility and inhibits arboviral replication in mosquitoes. This study aimed to assess Wolbachia prevalence and genetic diversity in different mosquito species from Cape Verde. Methods: Mosquitoes were collected on six islands of Cape Verde and identified to species using morphological keys and PCR-based assays. Wolbachia was detected by amplifying a fragment of the surface protein gene (wsp). Multilocus sequence typing (MLST) was performed with five housekeeping genes (coxA, gatB, ftsZ, hcpA, and fbpA) and the wsp hypervariable region (HVR) for strain identification. Identification of wPip groups (wPip-I to wPip-V) was performed using PCR–restriction fragment length polymorphism (RFLP) assay on the ankyrin domain gene pk1. Results: Nine mosquito species were collected, including the major vectors Aedes aegypti, Anopheles arabiensis, Culex pipiens sensu stricto, and Culex quinquefasciatus. Wolbachia was only detected in Cx. pipiens s.s. (100% prevalence), Cx. quinquefasciatus (98.3%), Cx. pipiens/quinquefasciatus hybrids (100%), and Culex tigripes (100%). Based on the results of MLST and wsp hypervariable region typing, Wolbachia from the Cx. pipiens complex was assigned to sequence type 9, wPip clade, and supergroup B. PCR/RFLP analysis revealed three wPip groups in Cape Verde, namely wPip-II, wPip-III, and wPip-IV. wPip-IV was the most prevalent, while wPip-II and wPip-III were found only on Maio and Fogo islands. Wolbachia detected in Cx. tigripes belongs to supergroup B, with no attributed MLST profile, indicating a new strain of Wolbachia in this mosquito species. Conclusions: A high prevalence and diversity of Wolbachia was found in species from the Cx. pipiens complex. This diversity may be related to the mosquito's colonization history on the Cape Verde islands. To the best of our knowledge, this is the first study to detect Wolbachia in Cx. tigripes, which may provide an additional opportunity for biocontrol initiatives. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/3c33a4ef-4759-48c3-8f79-5322601c67d1/download |
| id | run_e7150e6cd09245687009cd9729fb00a2 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/154352 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/154352 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | da Moura, Aires Januário Fernandes Valadas, Vera da Veiga Leal, Silvania Montalvo Sabino, Eddyson Sousa, Carla A. Pinto, João |
| publishDate | 2023 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenBackground: Wolbachia pipientis is an endosymbiont bacterium that induces cytoplasmic incompatibility and inhibits arboviral replication in mosquitoes. This study aimed to assess Wolbachia prevalence and genetic diversity in different mosquito species from Cape Verde. Methods: Mosquitoes were collected on six islands of Cape Verde and identified to species using morphological keys and PCR-based assays. Wolbachia was detected by amplifying a fragment of the surface protein gene (wsp). Multilocus sequence typing (MLST) was performed with five housekeeping genes (coxA, gatB, ftsZ, hcpA, and fbpA) and the wsp hypervariable region (HVR) for strain identification. Identification of wPip groups (wPip-I to wPip-V) was performed using PCR–restriction fragment length polymorphism (RFLP) assay on the ankyrin domain gene pk1. Results: Nine mosquito species were collected, including the major vectors Aedes aegypti, Anopheles arabiensis, Culex pipiens sensu stricto, and Culex quinquefasciatus. Wolbachia was only detected in Cx. pipiens s.s. (100% prevalence), Cx. quinquefasciatus (98.3%), Cx. pipiens/quinquefasciatus hybrids (100%), and Culex tigripes (100%). Based on the results of MLST and wsp hypervariable region typing, Wolbachia from the Cx. pipiens complex was assigned to sequence type 9, wPip clade, and supergroup B. PCR/RFLP analysis revealed three wPip groups in Cape Verde, namely wPip-II, wPip-III, and wPip-IV. wPip-IV was the most prevalent, while wPip-II and wPip-III were found only on Maio and Fogo islands. Wolbachia detected in Cx. tigripes belongs to supergroup B, with no attributed MLST profile, indicating a new strain of Wolbachia in this mosquito species. Conclusions: A high prevalence and diversity of Wolbachia was found in species from the Cx. pipiens complex. This diversity may be related to the mosquito's colonization history on the Cape Verde islands. To the best of our knowledge, this is the first study to detect Wolbachia in Cx. tigripes, which may provide an additional opportunity for biocontrol initiatives.application/pdfenScreening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islandsda Moura, Aires Januário FernandesValadas, Verada Veiga Leal, SilvaniaMontalvo Sabino, EddysonSousa, Carla A.Pinto, JoãoInstituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)BioMed Central (BMC)HostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1756-3305URNIsPartOfPURE: 63839441URNIsPartOfPURE UUID: 20ece78b-e980-469b-b274-f7d70de11c01URNIsPartOfScopus: 85153806060URNIsPartOfPubMed: 37098535URNIsPartOfPubMedCentral: 10131387URNIsPartOfWOS: 000978991500002URNIsPartOfORCID: /0000-0001-8572-7708/work/151424913DOIIsPartOf10.1186/s13071-023-05745-w2023-06-23T22:24:08Z2023-122023-12-01T00:00:00ZHandlehttp://hdl.handle.net/10362/154352http://purl.org/coar/access_right/c_abf2open accessCape VerdeCulex pipiensCulex tigripesGenotypingMosquitoesWolbachiaRA Public aspects of medicineQR MicrobiologyParasitologyGeneral VeterinaryInfectious DiseasesSDG 3 - Good Health and Well-being1745275 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/3c33a4ef-4759-48c3-8f79-5322601c67d1/download |
| spellingShingle | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands da Moura, Aires Januário Fernandes Cape Verde Culex pipiens Culex tigripes Genotyping Mosquitoes Wolbachia RA Public aspects of medicine QR Microbiology Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | Cape Verde Culex pipiens Culex tigripes Genotyping Mosquitoes Wolbachia RA Public aspects of medicine QR Microbiology Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being |
| title | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| title_full | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| title_fullStr | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| title_full_unstemmed | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| title_short | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| title_sort | Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands |
| topic | Cape Verde Culex pipiens Culex tigripes Genotyping Mosquitoes Wolbachia RA Public aspects of medicine QR Microbiology Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being |
| topic_facet | Cape Verde Culex pipiens Culex tigripes Genotyping Mosquitoes Wolbachia RA Public aspects of medicine QR Microbiology Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/154352 |
| visible | 1 |