Publicação
Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus
| Resumo: | Background: Culex quinquefasciatus plays a crucial role as a vector of West Nile virus (WNV). This mosquito species is widely distributed in Cape Verde, being found in all inhabited islands of the archipelago. However, no data are currently available on the susceptibility of the local mosquito population to WNV. This study aimed to assess the vector competence of Cx. quinquefasciatus mosquitoes from Santiago Island, Cape Verde, for WNV and to explore the potential impact of its native Wolbachia on virus transmission. Methods: Wolbachia-infected and uninfected Cx. quinquefasciatus female mosquitoes were exposed to WNV lineage 1 PT6.39 strain using a Hemotek membrane feeding system. Mosquito samples, including the body, legs, wings and saliva, were collected at days 7, 14 and 21 post-infection (dpi) to assess WNV infection through one-step quantitative real-time PCR (RT-qPCR). Results: Culex quinquefasciatus from Cape Verde exhibited high susceptibility to the tested strain of WNV. Also, treated females without their native Wolbachia exhibited significantly higher WNV load in their bodies and greater dissemination rate at 7 dpi than their wild-type counterparts carrying Wolbachia. Conclusions: The high susceptibility to WNV of Cx. quinquefasciatus from Cape Verde poses a potential risk for virus transmission in the archipelago. However, Wolbachia infection in this mosquito species seems to confer protection against WNV dissemination in the early stages of viral infection. Additional research is required to uncover the mechanisms driving this protection and its potential impact on WNV transmission. |
|---|---|
| Autores principais: | da Moura, Aires Januário Fernandes |
| Outros Autores: | Tomaz, Filipe; Melo, Tiago; Seixas, Gonçalo; Sousa, Carla A.; Pinto, João |
| Assunto: | Cape Verde Culexquinquefasciatus Vector competence West Nile virus Wolbachia Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being SDG 17 - Partnerships for the Goals |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983898664337408 |
|---|---|
| author | da Moura, Aires Januário Fernandes |
| author2 | Tomaz, Filipe Melo, Tiago Seixas, Gonçalo Sousa, Carla A. Pinto, João |
| author2_role | author author author author author |
| author_facet | da Moura, Aires Januário Fernandes Tomaz, Filipe Melo, Tiago Seixas, Gonçalo Sousa, Carla A. Pinto, João |
| author_role | author |
| contributor_name_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT) Vector borne diseases and pathogens (VBD) BioMed Central (BMC) RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"da Moura, Aires Januário Fernandes\"},{\"Person.name\":\"Tomaz, Filipe\"},{\"Person.name\":\"Melo, Tiago\"},{\"Person.name\":\"Seixas, Gonçalo\"},{\"Person.name\":\"Sousa, Carla A.\"},{\"Person.name\":\"Pinto, João\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT) Vector borne diseases and pathogens (VBD) BioMed Central (BMC) RUN |
| datacite.creators.creator.creatorName.fl_str_mv | da Moura, Aires Januário Fernandes Tomaz, Filipe Melo, Tiago Seixas, Gonçalo Sousa, Carla A. Pinto, João |
| datacite.date.Accepted.fl_str_mv | 2024-12-23T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-02-04T21:19:07Z |
| datacite.date.embargoed.fl_str_mv | 2025-02-04T21:19:07Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cape Verde Culexquinquefasciatus Vector competence West Nile virus Wolbachia Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being SDG 17 - Partnerships for the Goals |
| datacite.titles.title.fl_str_mv | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus exploring the potential effect of the vector native Wolbachia |
| dc.contributor.none.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT) Vector borne diseases and pathogens (VBD) BioMed Central (BMC) RUN |
| dc.creator.none.fl_str_mv | da Moura, Aires Januário Fernandes Tomaz, Filipe Melo, Tiago Seixas, Gonçalo Sousa, Carla A. Pinto, João |
| dc.date.Accepted.fl_str_mv | 2024-12-23T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-02-04T21:19:07Z |
| dc.date.embargoed.fl_str_mv | 2025-02-04T21:19:07Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/178424 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cape Verde Culexquinquefasciatus Vector competence West Nile virus Wolbachia Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being SDG 17 - Partnerships for the Goals |
| dc.title.fl_str_mv | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus exploring the potential effect of the vector native Wolbachia |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: Culex quinquefasciatus plays a crucial role as a vector of West Nile virus (WNV). This mosquito species is widely distributed in Cape Verde, being found in all inhabited islands of the archipelago. However, no data are currently available on the susceptibility of the local mosquito population to WNV. This study aimed to assess the vector competence of Cx. quinquefasciatus mosquitoes from Santiago Island, Cape Verde, for WNV and to explore the potential impact of its native Wolbachia on virus transmission. Methods: Wolbachia-infected and uninfected Cx. quinquefasciatus female mosquitoes were exposed to WNV lineage 1 PT6.39 strain using a Hemotek membrane feeding system. Mosquito samples, including the body, legs, wings and saliva, were collected at days 7, 14 and 21 post-infection (dpi) to assess WNV infection through one-step quantitative real-time PCR (RT-qPCR). Results: Culex quinquefasciatus from Cape Verde exhibited high susceptibility to the tested strain of WNV. Also, treated females without their native Wolbachia exhibited significantly higher WNV load in their bodies and greater dissemination rate at 7 dpi than their wild-type counterparts carrying Wolbachia. Conclusions: The high susceptibility to WNV of Cx. quinquefasciatus from Cape Verde poses a potential risk for virus transmission in the archipelago. However, Wolbachia infection in this mosquito species seems to confer protection against WNV dissemination in the early stages of viral infection. Additional research is required to uncover the mechanisms driving this protection and its potential impact on WNV transmission. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/07c46b6d-a413-4abb-976c-bcdb0bdf42c2/download |
| id | run_e8b58c73e1213ecde778046dbf4bb00a |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/178424 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/178424 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | da Moura, Aires Januário Fernandes Tomaz, Filipe Melo, Tiago Seixas, Gonçalo Sousa, Carla A. Pinto, João |
| publishDate | 2024 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenBackground: Culex quinquefasciatus plays a crucial role as a vector of West Nile virus (WNV). This mosquito species is widely distributed in Cape Verde, being found in all inhabited islands of the archipelago. However, no data are currently available on the susceptibility of the local mosquito population to WNV. This study aimed to assess the vector competence of Cx. quinquefasciatus mosquitoes from Santiago Island, Cape Verde, for WNV and to explore the potential impact of its native Wolbachia on virus transmission. Methods: Wolbachia-infected and uninfected Cx. quinquefasciatus female mosquitoes were exposed to WNV lineage 1 PT6.39 strain using a Hemotek membrane feeding system. Mosquito samples, including the body, legs, wings and saliva, were collected at days 7, 14 and 21 post-infection (dpi) to assess WNV infection through one-step quantitative real-time PCR (RT-qPCR). Results: Culex quinquefasciatus from Cape Verde exhibited high susceptibility to the tested strain of WNV. Also, treated females without their native Wolbachia exhibited significantly higher WNV load in their bodies and greater dissemination rate at 7 dpi than their wild-type counterparts carrying Wolbachia. Conclusions: The high susceptibility to WNV of Cx. quinquefasciatus from Cape Verde poses a potential risk for virus transmission in the archipelago. However, Wolbachia infection in this mosquito species seems to confer protection against WNV dissemination in the early stages of viral infection. Additional research is required to uncover the mechanisms driving this protection and its potential impact on WNV transmission.application/pdfenVector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile VirusSubtitleenexploring the potential effect of the vector native Wolbachiada Moura, Aires Januário FernandesTomaz, FilipeMelo, TiagoSeixas, GonçaloSousa, Carla A.Pinto, JoãoInstituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT)Vector borne diseases and pathogens (VBD)BioMed Central (BMC)HostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1756-3305URNIsPartOfPURE: 108622382URNIsPartOfPURE UUID: a0ff2f5b-28f6-456b-89f8-18b292c55c35URNIsPartOfScopus: 85212796441URNIsPartOfWOS: 001382744500006URNIsPartOfPubMed: 39716303URNIsPartOfPubMedCentral: PMC11668078URNIsPartOfORCID: /0000-0001-8572-7708/work/177366568DOIIsPartOf10.1186/s13071-024-06609-72025-02-04T21:19:07Z2024-12-232024-12-23T00:00:00ZHandlehttp://hdl.handle.net/10362/178424http://purl.org/coar/access_right/c_abf2open accessCape VerdeCulexquinquefasciatusVector competenceWest Nile virusWolbachiaParasitologyGeneral VeterinaryInfectious DiseasesSDG 3 - Good Health and Well-beingSDG 17 - Partnerships for the Goals1459840 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/07c46b6d-a413-4abb-976c-bcdb0bdf42c2/download |
| spellingShingle | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus da Moura, Aires Januário Fernandes Cape Verde Culexquinquefasciatus Vector competence West Nile virus Wolbachia Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being SDG 17 - Partnerships for the Goals |
| status | SINGLETON |
| subject.fl_str_mv | Cape Verde Culexquinquefasciatus Vector competence West Nile virus Wolbachia Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being SDG 17 - Partnerships for the Goals |
| title | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus |
| title_full | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus |
| title_fullStr | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus |
| title_full_unstemmed | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus |
| title_short | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus |
| title_sort | Vector competence of Culex quinquefasciatus from Santiago Island, Cape Verde, to West Nile Virus |
| topic | Cape Verde Culexquinquefasciatus Vector competence West Nile virus Wolbachia Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being SDG 17 - Partnerships for the Goals |
| topic_facet | Cape Verde Culexquinquefasciatus Vector competence West Nile virus Wolbachia Parasitology General Veterinary Infectious Diseases SDG 3 - Good Health and Well-being SDG 17 - Partnerships for the Goals |
| url | http://hdl.handle.net/10362/178424 |
| visible | 1 |