Publicação
Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells
| Resumo: | Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand. © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro. |
|---|---|
| Autores principais: | Oliveira, E. |
| Outros Autores: | Santos, H. M.; Garcia-Pardo, Javier; Diniz, M.; Lorenzo, Julia; Rodríguez-González, B.; Capelo, J. L.; Lodeiro, C. |
| Assunto: | Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868415344646815744 |
|---|---|
| author | Oliveira, E. |
| author2 | Santos, H. M. Garcia-Pardo, Javier Diniz, M. Lorenzo, Julia Rodríguez-González, B. Capelo, J. L. Lodeiro, C. |
| author2_role | author author author author author author author |
| author_facet | Oliveira, E. Santos, H. M. Garcia-Pardo, Javier Diniz, M. Lorenzo, Julia Rodríguez-González, B. Capelo, J. L. Lodeiro, C. |
| author_role | author |
| contributor_name_str_mv | DQ - Departamento de Química CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE) Frontiers Media RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Oliveira, E.\"},{\"Person.name\":\"Santos, H. M.\"},{\"Person.name\":\"Garcia-Pardo, Javier\"},{\"Person.name\":\"Diniz, M.\"},{\"Person.name\":\"Lorenzo, Julia\"},{\"Person.name\":\"Rodríguez-González, B.\"},{\"Person.name\":\"Capelo, J. L.\"},{\"Person.name\":\"Lodeiro, C.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | DQ - Departamento de Química CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE) Frontiers Media RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Oliveira, E. Santos, H. M. Garcia-Pardo, Javier Diniz, M. Lorenzo, Julia Rodríguez-González, B. Capelo, J. L. Lodeiro, C. |
| datacite.date.Accepted.fl_str_mv | 2013-12-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-07-19T22:09:07Z |
| datacite.date.embargoed.fl_str_mv | 2018-07-19T22:09:07Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles |
| datacite.titles.title.fl_str_mv | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| dc.contributor.none.fl_str_mv | DQ - Departamento de Química CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE) Frontiers Media RUN |
| dc.creator.none.fl_str_mv | Oliveira, E. Santos, H. M. Garcia-Pardo, Javier Diniz, M. Lorenzo, Julia Rodríguez-González, B. Capelo, J. L. Lodeiro, C. |
| dc.date.Accepted.fl_str_mv | 2013-12-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-07-19T22:09:07Z |
| dc.date.embargoed.fl_str_mv | 2018-07-19T22:09:07Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/42017 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles |
| dc.title.fl_str_mv | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand. © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/c48e4770-1424-484a-9512-02164f99a108/download |
| id | run_e8b90c62cd8181deea9ae32eb82efabc |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/42017 |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/42017 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Oliveira, E. Santos, H. M. Garcia-Pardo, Javier Diniz, M. Lorenzo, Julia Rodríguez-González, B. Capelo, J. L. Lodeiro, C. |
| publishDate | 2013 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenSilver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand. © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro.application/pdfenSynthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cellsOliveira, E.Santos, H. M.Garcia-Pardo, JavierDiniz, M.Lorenzo, JuliaRodríguez-González, B.Capelo, J. L.Lodeiro, C.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)Frontiers MediaHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2296-2646URNIsPartOfPURE: 2587433URNIsPartOfPURE UUID: b357f23d-6265-40c1-a286-24f252fe14e7URNIsPartOfRIS: urn:A82A4B3DF2435805F3F6F3E8F2A0A23FURNIsPartOfScopus: 84987753775URNIsPartOfWOS: 000209678500027URNIsPartOfPubMed: 24790957URNIsPartOfPubMedCentral: PMC3988373URNIsPartOfORCID: /0000-0001-5582-5446/work/46943974URNIsPartOfORCID: /0000-0002-6032-8679/work/55907709DOIIsPartOf10.3389/fchem.2013.000292018-07-19T22:09:07Z2013-122013-12-01T00:00:00ZHandlehttp://hdl.handle.net/10362/42017http://purl.org/coar/access_right/c_abf2open accessCytotoxicityFluorescenceGoldfishHuman hepatoma cellsSilver nanoparticles2647818 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/c48e4770-1424-484a-9512-02164f99a108/download |
| spellingShingle | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells Oliveira, E. Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles |
| status | SINGLETON |
| subject.fl_str_mv | Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles |
| title | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| title_full | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| title_fullStr | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| title_full_unstemmed | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| title_short | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| title_sort | Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells |
| topic | Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles |
| topic_facet | Cytotoxicity Fluorescence Goldfish Human hepatoma cells Silver nanoparticles |
| url | http://hdl.handle.net/10362/42017 |
| visible | 1 |