Publicação
A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina
| Resumo: | The current study investigates the venom-delivery system of green and red morphotypes of the sea anemone Actinia equina to disclose its potential as a source of bioactive compounds. We compared the two morphotypes using electron and optical microscopy, proteomics, and toxicity assessment on zebrafish embryos. Specialized venom-injecting cells (nematocysts) are equally distributed and found in the tentacles of both varieties. Proteomics revealed proteins of interest in both red and green Actinia, yielding the three most abundant Gene Ontology (GO) terms related to the biological processes “proteolysis”, “hemolysis in another organism” and “lipid catabolic process”. Neurotoxins and cytolytic toxins similar to known cnidarian toxins like PsTX-60A and AvTX-60A, for instance, were identified in both types. Extracts from green and red anemones were toxic to zebrafish embryos, with green anemone venom appearing to be more potent. The findings highlight the presence of proteinaceous toxins in A. equina and the potential for different varieties to possess distinct bioactive compounds. Notably, pore-forming toxins are suggested for molecular probes and immunotoxins, making them valuable assets for potential biotechnological and biomedical purposes. |
|---|---|
| Autores principais: | Alcaide, Maria |
| Outros Autores: | Moutinho Cabral, Inês; Carvalho, Lara; Mendes, Vera M.; Alves de Matos, António P.; Manadas, Bruno; Saúde, Leonor; D’Ambrosio, Mariaelena; Costa, Pedro M. |
| Assunto: | bioinformatics cnidaria electron microscopy histology marine bioprospecting proteomics toxicity toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983452311748608 |
|---|---|
| author | Alcaide, Maria |
| author2 | Moutinho Cabral, Inês Carvalho, Lara Mendes, Vera M. Alves de Matos, António P. Manadas, Bruno Saúde, Leonor D’Ambrosio, Mariaelena Costa, Pedro M. |
| author2_role | author author author author author author author author |
| author_facet | Alcaide, Maria Moutinho Cabral, Inês Carvalho, Lara Mendes, Vera M. Alves de Matos, António P. Manadas, Bruno Saúde, Leonor D’Ambrosio, Mariaelena Costa, Pedro M. |
| author_role | author |
| contributor_name_str_mv | DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit MDPI - Multidisciplinary Digital Publishing Institute RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Alcaide, Maria\"},{\"Person.name\":\"Moutinho Cabral, Inês\"},{\"Person.name\":\"Carvalho, Lara\"},{\"Person.name\":\"Mendes, Vera M.\"},{\"Person.name\":\"Alves de Matos, António P.\"},{\"Person.name\":\"Manadas, Bruno\"},{\"Person.name\":\"Saúde, Leonor\"},{\"Person.name\":\"D’Ambrosio, Mariaelena\"},{\"Person.name\":\"Costa, Pedro M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit MDPI - Multidisciplinary Digital Publishing Institute RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Alcaide, Maria Moutinho Cabral, Inês Carvalho, Lara Mendes, Vera M. Alves de Matos, António P. Manadas, Bruno Saúde, Leonor D’Ambrosio, Mariaelena Costa, Pedro M. |
| datacite.date.Accepted.fl_str_mv | 2024-03-21T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-04-17T01:24:40Z |
| datacite.date.embargoed.fl_str_mv | 2024-04-17T01:24:40Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | bioinformatics cnidaria electron microscopy histology marine bioprospecting proteomics toxicity toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water |
| datacite.titles.title.fl_str_mv | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| dc.contributor.none.fl_str_mv | DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit MDPI - Multidisciplinary Digital Publishing Institute RUN |
| dc.creator.none.fl_str_mv | Alcaide, Maria Moutinho Cabral, Inês Carvalho, Lara Mendes, Vera M. Alves de Matos, António P. Manadas, Bruno Saúde, Leonor D’Ambrosio, Mariaelena Costa, Pedro M. |
| dc.date.Accepted.fl_str_mv | 2024-03-21T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-04-17T01:24:40Z |
| dc.date.embargoed.fl_str_mv | 2024-04-17T01:24:40Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/166304 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | bioinformatics cnidaria electron microscopy histology marine bioprospecting proteomics toxicity toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water |
| dc.title.fl_str_mv | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The current study investigates the venom-delivery system of green and red morphotypes of the sea anemone Actinia equina to disclose its potential as a source of bioactive compounds. We compared the two morphotypes using electron and optical microscopy, proteomics, and toxicity assessment on zebrafish embryos. Specialized venom-injecting cells (nematocysts) are equally distributed and found in the tentacles of both varieties. Proteomics revealed proteins of interest in both red and green Actinia, yielding the three most abundant Gene Ontology (GO) terms related to the biological processes “proteolysis”, “hemolysis in another organism” and “lipid catabolic process”. Neurotoxins and cytolytic toxins similar to known cnidarian toxins like PsTX-60A and AvTX-60A, for instance, were identified in both types. Extracts from green and red anemones were toxic to zebrafish embryos, with green anemone venom appearing to be more potent. The findings highlight the presence of proteinaceous toxins in A. equina and the potential for different varieties to possess distinct bioactive compounds. Notably, pore-forming toxins are suggested for molecular probes and immunotoxins, making them valuable assets for potential biotechnological and biomedical purposes. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/57149c3e-80a3-4498-99eb-627dd947fa78/download |
| funder_facet_str_mv | FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia FCT{{{_:::_}}}Fundação para a Ciência e a Tecnologia |
| funding.funder.alternateName_str_mv | FCT FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID |
| id | run_ea9111fbc40f78bcce73518b69c8aa2f |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/166304 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
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| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/166304 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Alcaide, Maria Moutinho Cabral, Inês Carvalho, Lara Mendes, Vera M. Alves de Matos, António P. Manadas, Bruno Saúde, Leonor D’Ambrosio, Mariaelena Costa, Pedro M. |
| publishDate | 2024 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
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| spelling | engenThe current study investigates the venom-delivery system of green and red morphotypes of the sea anemone Actinia equina to disclose its potential as a source of bioactive compounds. We compared the two morphotypes using electron and optical microscopy, proteomics, and toxicity assessment on zebrafish embryos. Specialized venom-injecting cells (nematocysts) are equally distributed and found in the tentacles of both varieties. Proteomics revealed proteins of interest in both red and green Actinia, yielding the three most abundant Gene Ontology (GO) terms related to the biological processes “proteolysis”, “hemolysis in another organism” and “lipid catabolic process”. Neurotoxins and cytolytic toxins similar to known cnidarian toxins like PsTX-60A and AvTX-60A, for instance, were identified in both types. Extracts from green and red anemones were toxic to zebrafish embryos, with green anemone venom appearing to be more potent. The findings highlight the presence of proteinaceous toxins in A. equina and the potential for different varieties to possess distinct bioactive compounds. Notably, pore-forming toxins are suggested for molecular probes and immunotoxins, making them valuable assets for potential biotechnological and biomedical purposes.application/pdfenA Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equinaAlcaide, MariaMoutinho Cabral, InêsCarvalho, LaraMendes, Vera M.Alves de Matos, António P.Manadas, BrunoSaúde, LeonorD’Ambrosio, MariaelenaCosta, Pedro M.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitMDPI - Multidisciplinary Digital Publishing InstituteHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf2076-2615URNIsPartOfPURE: 87469604URNIsPartOfPURE UUID: 50e32ecf-6901-497b-a7d1-edd0714725a8URNIsPartOfScopus: 85188816379URNIsPartOfWOS: 001191879000001URNIsPartOfPubMed: 38540078URNIsPartOfPubMedCentral: PMC10967595DOIIsPartOf10.3390/ani140609812024-04-17T01:24:40Z2024-03-212024-03-21T00:00:00ZHandlehttp://hdl.handle.net/10362/166304http://purl.org/coar/access_right/c_abf2open accessbioinformaticscnidariaelectron microscopyhistologymarine bioprospectingproteomicstoxicitytoxinsAnimal Science and ZoologyGeneral VeterinarySDG 14 - Life Below Water6897444 bytesFundação para a Ciência e a TecnologiaApplied Molecular Biosciences Unit6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaInstitute for Health and Bioeconomy6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCenter for Innovative Biomedicine and Biotechnology6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCenter for Innovative Biomedicine and Biotechnology6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCenter for Innovative Biomedicine and Biotechnology - Associate Laboratory6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/57149c3e-80a3-4498-99eb-627dd947fa78/download |
| spellingShingle | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina Alcaide, Maria bioinformatics cnidaria electron microscopy histology marine bioprospecting proteomics toxicity toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water |
| status | SINGLETON |
| subject.fl_str_mv | bioinformatics cnidaria electron microscopy histology marine bioprospecting proteomics toxicity toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water |
| title | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| title_full | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| title_fullStr | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| title_full_unstemmed | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| title_short | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| title_sort | A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina |
| topic | bioinformatics cnidaria electron microscopy histology marine bioprospecting proteomics toxicity toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water |
| topic_facet | bioinformatics cnidaria electron microscopy histology marine bioprospecting proteomics toxicity toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water |
| url | http://hdl.handle.net/10362/166304 |
| visible | 1 |