Publicação
In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration
| Resumo: | Background: Zebrafish (Danio rerio) has a remarkable capacity to regenerate many organs and tissues. During larval stages the fin fold allows the possibility of performing long time-lapse imaging making this system very appealing to study the relationships between tissue movements, cell migration and proliferation necessary for the regeneration process. Results: Through the combined use of transgenic fluorescently-labeled animals and confocal microscopy imaging, we characterized in vivo the complete fin fold regeneration process. We show, for the first time, that there is an increase in the global rate of epidermal growth as a response to tissue loss. Also enhanced significantly is cell proliferation, which upon amputation happens in a broad area concerning the amputation level and not in a blastema-restricted way. This reveals a striking difference with regard to the adult fin regeneration system. Finally, an accumulation of migratory, shape-changing fibroblasts occurs proximally to the wound area, resembling a blastemal-like structure, which may act as a signaling center for the regeneration process to proceed. Conclusions: These findings provide a novel in vivo description of fundamental mechanisms occurring during the fin fold regeneration process, thereby contributing to a better knowledge of this regenerative system and to reveal variations in the epimorphic regeneration field. |
|---|---|
| Autores principais: | Mateus, Rita |
| Outros Autores: | Pereira, Telmo; Sousa, Sara; de Lima, Joana Esteves; Pascoal, Susana; Saúde, Leonor; Jacinto, Antonio; Jacinto, Antonio |
| Assunto: | AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1865920626612502528 |
|---|---|
| author | Mateus, Rita |
| author2 | Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio Jacinto, Antonio |
| author2_role | author author author author author author author |
| author_facet | Mateus, Rita Mateus, Rita Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio Jacinto, Antonio Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio Jacinto, Antonio |
| author_role | author |
| contributor_name_str_mv | Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) PLOS - Public Library of Science RUN |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Mateus, Rita\"},{\"Person.name\":\"Pereira, Telmo\"},{\"Person.name\":\"Sousa, Sara\"},{\"Person.name\":\"de Lima, Joana Esteves\"},{\"Person.name\":\"Pascoal, Susana\"},{\"Person.name\":\"Saúde, Leonor\"},{\"Person.name\":\"Jacinto, Antonio\"},{\"Person.name\":\"Jacinto, Antonio\",\"Person.identifier.orcid\":\"0000-0002-4193-6089\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) PLOS - Public Library of Science RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Mateus, Rita Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio Jacinto, Antonio |
| datacite.date.Accepted.fl_str_mv | 2012-12-20T00:00:00Z |
| datacite.date.available.fl_str_mv | 2017-08-03T22:05:04Z |
| datacite.date.embargoed.fl_str_mv | 2017-08-03T22:05:04Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences |
| datacite.titles.title.fl_str_mv | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| dc.contributor.none.fl_str_mv | Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) PLOS - Public Library of Science RUN |
| dc.creator.none.fl_str_mv | Mateus, Rita Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio Jacinto, Antonio |
| dc.date.Accepted.fl_str_mv | 2012-12-20T00:00:00Z |
| dc.date.available.fl_str_mv | 2017-08-03T22:05:04Z |
| dc.date.embargoed.fl_str_mv | 2017-08-03T22:05:04Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://www.scopus.com/inward/record.url?scp=84871414741&partnerID=8YFLogxK |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences |
| dc.title.fl_str_mv | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: Zebrafish (Danio rerio) has a remarkable capacity to regenerate many organs and tissues. During larval stages the fin fold allows the possibility of performing long time-lapse imaging making this system very appealing to study the relationships between tissue movements, cell migration and proliferation necessary for the regeneration process. Results: Through the combined use of transgenic fluorescently-labeled animals and confocal microscopy imaging, we characterized in vivo the complete fin fold regeneration process. We show, for the first time, that there is an increase in the global rate of epidermal growth as a response to tissue loss. Also enhanced significantly is cell proliferation, which upon amputation happens in a broad area concerning the amputation level and not in a blastema-restricted way. This reveals a striking difference with regard to the adult fin regeneration system. Finally, an accumulation of migratory, shape-changing fibroblasts occurs proximally to the wound area, resembling a blastemal-like structure, which may act as a signaling center for the regeneration process to proceed. Conclusions: These findings provide a novel in vivo description of fundamental mechanisms occurring during the fin fold regeneration process, thereby contributing to a better knowledge of this regenerative system and to reveal variations in the epimorphic regeneration field. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://run.unl.pt/bitstreams/b5adfc3a-d438-4476-ab4b-c1770ecd4dfe/download |
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| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
| network_acronym_str | run |
| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/22459 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Mateus, Rita Pereira, Telmo Sousa, Sara de Lima, Joana Esteves Pascoal, Susana Saúde, Leonor Jacinto, Antonio Jacinto, Antonio Jacinto, Antonio https://www.ciencia-id.pt/DF15-1DEB-A48C DF15-1DEB-A48C http://orcid.org/0000-0002-4193-6089 0000-0002-4193-6089 |
| publishDate | 2012 |
| reponame_str | Repositório Institucional da UNL |
| repository_id_str | urn:repositoryAcronym:run |
| service_str_mv | urn:repositoryAcronym:run |
| spelling | engenBackground: Zebrafish (Danio rerio) has a remarkable capacity to regenerate many organs and tissues. During larval stages the fin fold allows the possibility of performing long time-lapse imaging making this system very appealing to study the relationships between tissue movements, cell migration and proliferation necessary for the regeneration process. Results: Through the combined use of transgenic fluorescently-labeled animals and confocal microscopy imaging, we characterized in vivo the complete fin fold regeneration process. We show, for the first time, that there is an increase in the global rate of epidermal growth as a response to tissue loss. Also enhanced significantly is cell proliferation, which upon amputation happens in a broad area concerning the amputation level and not in a blastema-restricted way. This reveals a striking difference with regard to the adult fin regeneration system. Finally, an accumulation of migratory, shape-changing fibroblasts occurs proximally to the wound area, resembling a blastemal-like structure, which may act as a signaling center for the regeneration process to proceed. Conclusions: These findings provide a novel in vivo description of fundamental mechanisms occurring during the fin fold regeneration process, thereby contributing to a better knowledge of this regenerative system and to reveal variations in the epimorphic regeneration field.application/pdfenIn Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold RegenerationMateus, RitaPereira, TelmoSousa, Sarade Lima, Joana EstevesPascoal, SusanaSaúde, LeonorJacinto, AntonioPersonalJacinto, AntonioDSpacehttp://dspace.org/items/37ff3a19-e7d7-4c9f-af75-fade2f9aa9d1DSpacehttp://dspace.org/items/37ff3a19-e7d7-4c9f-af75-fade2f9aa9d1JacintoAntonioCiência IDhttps://www.ciencia-id.ptDF15-1DEB-A48CORCIDhttp://orcid.org0000-0002-4193-6089Researcher IDhttps://www.researcherid.comF-5729-2013Scopus Author IDhttps://www.scopus.com55880355900Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)PLOS - Public Library of ScienceHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1932-6203URNIsPartOfPURE: 2991045URNIsPartOfPURE UUID: e7dd3cd1-e51e-4d32-988e-0096b9cd8020URNIsPartOfScopus: 84871414741URNIsPartOfPubMed: 23284763URNIsPartOfWOS: 000312794500044URNIsPartOfORCID: /0000-0002-4193-6089/work/45330342DOIIsPartOf10.1371/journal.pone.00517662017-08-03T22:05:04Z2012-12-202012-12-20T00:00:00ZURLhttp://www.scopus.com/inward/record.url?scp=84871414741&partnerID=8YFLogxKhttp://purl.org/coar/access_right/c_abf2open accessAXOLOTL LIMB REGENERATIONFUNCTIONAL-ANALYSISBLASTEMA FORMATIONGENE-EXPRESSIONFUSION PROTEINTELEOST FINMORPHOGENESISDEDIFFERENTIATIONPROLIFERATIONGASTRULATIONGeneral MedicineGeneral Biochemistry,Genetics and Molecular BiologyGeneral Agricultural and Biological Sciences4880037 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/b5adfc3a-d438-4476-ab4b-c1770ecd4dfe/download |
| spellingShingle | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration Mateus, Rita AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences Mateus, Rita AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences |
| status | NEW |
| subject.fl_str_mv | AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences |
| title | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| title_full | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| title_fullStr | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| title_full_unstemmed | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| title_short | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| title_sort | In Vivo Cell and Tissue Dynamics Underlying Zebrafish Fin Fold Regeneration |
| topic | AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences |
| topic_facet | AXOLOTL LIMB REGENERATION FUNCTIONAL-ANALYSIS BLASTEMA FORMATION GENE-EXPRESSION FUSION PROTEIN TELEOST FIN MORPHOGENESIS DEDIFFERENTIATION PROLIFERATION GASTRULATION General Medicine General Biochemistry,Genetics and Molecular Biology General Agricultural and Biological Sciences |
| url | http://www.scopus.com/inward/record.url?scp=84871414741&partnerID=8YFLogxK |
| visible | 1 |