Publicação
Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice
| Resumo: | Background: In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Methodology/Principal Findings: Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p<0.001), a longer patent period (p<0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p<0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p<0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. Conclusion/Significance: T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice. |
|---|---|
| Autores principais: | Monteiro, Wuelton Marcelo |
| Outros Autores: | Margioto Teston, Ana Paula; Gruendling, Ana Paula; dos Reis, Daniele; Gomes, Mônica Lúcia; Marques de Araújo, Silvana; Bahia, Maria Terezinha; Costa Magalhães, Laylah Kelre; de Oliveira Guerra, Jorge Augusto; Silveira, Henrique; de Ornelas Toledo, Max Jean; Vale Barbosa, Maria das Graças |
| Assunto: | Infectious Diseases Epidemiology Parasitology SDG 3 - Good Health and Well-being |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Nova de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório Institucional da UNL |
| _version_ | 1868983328655278080 |
|---|---|
| author | Monteiro, Wuelton Marcelo |
| author2 | Margioto Teston, Ana Paula Gruendling, Ana Paula dos Reis, Daniele Gomes, Mônica Lúcia Marques de Araújo, Silvana Bahia, Maria Terezinha Costa Magalhães, Laylah Kelre de Oliveira Guerra, Jorge Augusto Silveira, Henrique de Ornelas Toledo, Max Jean Vale Barbosa, Maria das Graças |
| author2_role | author author author author author author author author author author author |
| author_facet | Monteiro, Wuelton Marcelo Margioto Teston, Ana Paula Gruendling, Ana Paula dos Reis, Daniele Gomes, Mônica Lúcia Marques de Araújo, Silvana Bahia, Maria Terezinha Costa Magalhães, Laylah Kelre de Oliveira Guerra, Jorge Augusto Silveira, Henrique de Ornelas Toledo, Max Jean Vale Barbosa, Maria das Graças |
| author_role | author |
| contributor_name_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) PLOS - Public Library of Science RUN |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Monteiro, Wuelton Marcelo\"},{\"Person.name\":\"Margioto Teston, Ana Paula\"},{\"Person.name\":\"Gruendling, Ana Paula\"},{\"Person.name\":\"dos Reis, Daniele\"},{\"Person.name\":\"Gomes, Mônica Lúcia\"},{\"Person.name\":\"Marques de Araújo, Silvana\"},{\"Person.name\":\"Bahia, Maria Terezinha\"},{\"Person.name\":\"Costa Magalhães, Laylah Kelre\"},{\"Person.name\":\"de Oliveira Guerra, Jorge Augusto\"},{\"Person.name\":\"Silveira, Henrique\"},{\"Person.name\":\"de Ornelas Toledo, Max Jean\"},{\"Person.name\":\"Vale Barbosa, Maria das Graças\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) PLOS - Public Library of Science RUN |
| datacite.creators.creator.creatorName.fl_str_mv | Monteiro, Wuelton Marcelo Margioto Teston, Ana Paula Gruendling, Ana Paula dos Reis, Daniele Gomes, Mônica Lúcia Marques de Araújo, Silvana Bahia, Maria Terezinha Costa Magalhães, Laylah Kelre de Oliveira Guerra, Jorge Augusto Silveira, Henrique de Ornelas Toledo, Max Jean Vale Barbosa, Maria das Graças |
| datacite.date.Accepted.fl_str_mv | 2013-02-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-05-04T22:55:43Z |
| datacite.date.embargoed.fl_str_mv | 2021-05-04T22:55:43Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Infectious Diseases Epidemiology Parasitology SDG 3 - Good Health and Well-being |
| datacite.titles.title.fl_str_mv | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| dc.contributor.none.fl_str_mv | Instituto de Higiene e Medicina Tropical (IHMT) PLOS - Public Library of Science RUN |
| dc.creator.none.fl_str_mv | Monteiro, Wuelton Marcelo Margioto Teston, Ana Paula Gruendling, Ana Paula dos Reis, Daniele Gomes, Mônica Lúcia Marques de Araújo, Silvana Bahia, Maria Terezinha Costa Magalhães, Laylah Kelre de Oliveira Guerra, Jorge Augusto Silveira, Henrique de Ornelas Toledo, Max Jean Vale Barbosa, Maria das Graças |
| dc.date.Accepted.fl_str_mv | 2013-02-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-05-04T22:55:43Z |
| dc.date.embargoed.fl_str_mv | 2021-05-04T22:55:43Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10362/117031 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Infectious Diseases Epidemiology Parasitology SDG 3 - Good Health and Well-being |
| dc.title.fl_str_mv | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Methodology/Principal Findings: Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p<0.001), a longer patent period (p<0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p<0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p<0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. Conclusion/Significance: T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
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| id | run_f7dc6e8bf7fbdd70b34d0e2cd34586d3 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10362/117031 |
| inst_facet_str | urn:organizationAcronym:unl{{{_:::_}}}Universidade Nova de Lisboa |
| instacron_str | unl |
| institution | Universidade Nova de Lisboa |
| instname_str | Universidade Nova de Lisboa |
| language | eng |
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| network_name_str | Repositório Institucional da UNL |
| oai_identifier_str | oai:run.unl.pt:10362/117031 |
| organization_str_mv | urn:organizationAcronym:unl |
| person_str_mv | Monteiro, Wuelton Marcelo Margioto Teston, Ana Paula Gruendling, Ana Paula dos Reis, Daniele Gomes, Mônica Lúcia Marques de Araújo, Silvana Bahia, Maria Terezinha Costa Magalhães, Laylah Kelre de Oliveira Guerra, Jorge Augusto Silveira, Henrique de Ornelas Toledo, Max Jean Vale Barbosa, Maria das Graças |
| publishDate | 2013 |
| repo_facet_str | urn:repositoryAcronym:run{{{_:::_}}}Repositório Institucional da UNL |
| reponame_str | Repositório Institucional da UNL |
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| spelling | engenBackground: In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Methodology/Principal Findings: Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p<0.001), a longer patent period (p<0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p<0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p<0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. Conclusion/Significance: T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice.application/pdfenTrypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in MiceMonteiro, Wuelton MarceloMargioto Teston, Ana PaulaGruendling, Ana Paulados Reis, DanieleGomes, Mônica LúciaMarques de Araújo, SilvanaBahia, Maria TerezinhaCosta Magalhães, Laylah Kelrede Oliveira Guerra, Jorge AugustoSilveira, Henriquede Ornelas Toledo, Max JeanVale Barbosa, Maria das GraçasInstituto de Higiene e Medicina Tropical (IHMT)PLOS - Public Library of ScienceHostingInstitutionOrganizationalRUNe-mailmailto:run@unl.ptrun@unl.ptISSNIsPartOf1935-2727URNIsPartOfPURE: 26755038URNIsPartOfPURE UUID: 3d03fa1f-aa1f-48ad-b2b5-53a0e1ecaa96URNIsPartOfScopus: 84874763840URNIsPartOfWOS: 000315644900033URNIsPartOfPubMed: 23437410URNIsPartOfORCID: /0000-0002-7939-772X/work/84883118DOIIsPartOf10.1371/journal.pntd.00020692021-05-04T22:55:43Z2013-022013-02-01T00:00:00ZHandlehttp://hdl.handle.net/10362/117031http://purl.org/coar/access_right/c_abf2open accessInfectious DiseasesEpidemiologyParasitologySDG 3 - Good Health and Well-being566653 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://run.unl.pt/bitstreams/7d78112e-84f0-456a-8f62-fcee1da62366/download |
| spellingShingle | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice Monteiro, Wuelton Marcelo Infectious Diseases Epidemiology Parasitology SDG 3 - Good Health and Well-being |
| status | SINGLETON |
| subject.fl_str_mv | Infectious Diseases Epidemiology Parasitology SDG 3 - Good Health and Well-being |
| title | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| title_full | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| title_fullStr | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| title_full_unstemmed | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| title_short | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| title_sort | Trypanosoma cruzi I and IV Stocks from Brazilian Amazon Are Divergent in Terms of Biological and Medical Properties in Mice |
| topic | Infectious Diseases Epidemiology Parasitology SDG 3 - Good Health and Well-being |
| topic_facet | Infectious Diseases Epidemiology Parasitology SDG 3 - Good Health and Well-being |
| url | http://hdl.handle.net/10362/117031 |
| visible | 1 |