Publicação
Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation
| Resumo: | Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp. |
|---|---|
| Autores principais: | Roberto, Vania Palma |
| Outros Autores: | Cavaco, S.; S B Viegas, Carla; Simes, D; Ortiz-Delgado, J. B.; Sarasquete, C.; Gavaia, Paulo J.; Cancela, Leonor |
| Assunto: | Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
| Ano: | 2009 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade do Algarve |
| Idioma: | inglês |
| Origem: | Sapientia - Universidade do Algarve |
| _version_ | 1867051124241989632 |
|---|---|
| author | Roberto, Vania Palma |
| author2 | Cavaco, S. S B Viegas, Carla Simes, D Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Cancela, Leonor |
| author2_role | author author author author author author author |
| author_facet | Roberto, Vania Palma Cavaco, S. S B Viegas, Carla Simes, D Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Cancela, Leonor |
| author_role | author |
| contributor_name_str_mv | Sapientia |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Roberto, Vania Palma\",\"Person.identifier.orcid\":\"0000-0002-8134-4045\"},{\"Person.name\":\"Cavaco, S.\"},{\"Person.name\":\"S B Viegas, Carla\",\"Person.identifier.orcid\":\"0000-0002-5765-3665\"},{\"Person.name\":\"Simes, D\",\"Person.identifier.orcid\":\"0000-0002-5145-4753\"},{\"Person.name\":\"Ortiz-Delgado, J. B.\"},{\"Person.name\":\"Sarasquete, C.\"},{\"Person.name\":\"Gavaia, Paulo J.\",\"Person.identifier.orcid\":\"0000-0002-9582-1957\"},{\"Person.name\":\"Cancela, Leonor\",\"Person.identifier.orcid\":\"0000-0003-3114-6662\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Sapientia |
| datacite.creators.creator.creatorName.fl_str_mv | Roberto, Vania Palma Cavaco, S. S B Viegas, Carla Simes, D Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Cancela, Leonor |
| datacite.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-06-04T13:28:35Z |
| datacite.date.embargoed.fl_str_mv | 2014-06-04T13:28:35Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
| datacite.titles.title.fl_str_mv | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| dc.contributor.none.fl_str_mv | Sapientia |
| dc.creator.none.fl_str_mv | Roberto, Vania Palma Cavaco, S. S B Viegas, Carla Simes, D Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Cancela, Leonor |
| dc.date.Accepted.fl_str_mv | 2009-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-06-04T13:28:35Z |
| dc.date.embargoed.fl_str_mv | 2014-06-04T13:28:35Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.1/4184 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
| dc.title.fl_str_mv | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://sapientia.ualg.pt/bitstreams/7d0922e4-23e5-4ee1-8e76-861d99da4232/download |
| id | sapientia_bf60065c93d718c19bbbca523a5e78cc |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.1/4184 |
| instacron_str | ualg |
| institution | Universidade do Algarve |
| instname_str | Universidade do Algarve |
| language | eng |
| network_acronym_str | sapientia |
| network_name_str | Sapientia - Universidade do Algarve |
| oai_identifier_str | oai:sapientia.ualg.pt:10400.1/4184 |
| organization_str_mv | urn:organizationAcronym:ualg |
| person_str_mv | Roberto, Vania Palma Roberto, Vania Palma https://www.ciencia-id.pt/681E-40B7-331F 681E-40B7-331F http://orcid.org/0000-0002-8134-4045 0000-0002-8134-4045 Cavaco, S. S B Viegas, Carla S B Viegas, Carla https://www.ciencia-id.pt/C414-F596-EEC6 C414-F596-EEC6 http://orcid.org/0000-0002-5765-3665 0000-0002-5765-3665 Simes, D Simes, D http://orcid.org/0000-0002-5145-4753 0000-0002-5145-4753 Ortiz-Delgado, J. B. Sarasquete, C. Gavaia, Paulo J. Gavaia, Paulo J. https://www.ciencia-id.pt/B619-FC16-D007 B619-FC16-D007 http://orcid.org/0000-0002-9582-1957 0000-0002-9582-1957 Cancela, Leonor Cancela, Leonor http://orcid.org/0000-0003-3114-6662 0000-0003-3114-6662 |
| publishDate | 2009 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Sapientia - Universidade do Algarve |
| repository_id_str | urn:repositoryAcronym:sapientia |
| service_str_mv | urn:repositoryAcronym:sapientia |
| spelling | engElsevierporMatrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.application/pdfporMatrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulationPersonalRoberto, Vania PalmaDSpacehttp://dspace.org/items/c730a868-0e9f-43e4-a354-10130b30b764DSpacehttp://dspace.org/items/c730a868-0e9f-43e4-a354-10130b30b764RobertoVâniaCiência IDhttps://www.ciencia-id.pt681E-40B7-331FORCIDhttp://orcid.org0000-0002-8134-4045Scopus Author IDhttps://www.scopus.com27467657800Cavaco, S.PersonalS B Viegas, CarlaDSpacehttp://dspace.org/items/33984b3c-ffac-477a-896a-ddbf7aced4e3DSpacehttp://dspace.org/items/33984b3c-ffac-477a-896a-ddbf7aced4e3ViegasCarlaCiência IDhttps://www.ciencia-id.ptC414-F596-EEC6ORCIDhttp://orcid.org0000-0002-5765-3665Researcher IDhttps://www.researcherid.comN-6695-2014Scopus Author IDhttps://www.scopus.com8656310300PersonalSimes, DDSpacehttp://dspace.org/items/31bac96d-1d01-4b8f-8f2b-9da4be31ea41DSpacehttp://dspace.org/items/31bac96d-1d01-4b8f-8f2b-9da4be31ea41SimesDinaORCIDhttp://orcid.org0000-0002-5145-4753Researcher IDhttps://www.researcherid.comN-2789-2014Scopus Author IDhttps://www.scopus.com7201884723Ortiz-Delgado, J. B.Sarasquete, C.PersonalGavaia, Paulo J.DSpacehttp://dspace.org/items/9dca2139-21a4-4d59-aaf7-531f1033a58eDSpacehttp://dspace.org/items/9dca2139-21a4-4d59-aaf7-531f1033a58eGavaiaPauloCiência IDhttps://www.ciencia-id.ptB619-FC16-D007ORCIDhttp://orcid.org0000-0002-9582-1957Researcher IDhttps://www.researcherid.comA-6470-2011Scopus Author IDhttps://www.scopus.com6507104377PersonalCancela, LeonorDSpacehttp://dspace.org/items/b9bbfe32-3dfe-4131-ad14-a4394008447fDSpacehttp://dspace.org/items/b9bbfe32-3dfe-4131-ad14-a4394008447fCancelaM. LeonorORCIDhttp://orcid.org0000-0003-3114-6662HostingInstitutionOrganizationalSapientiae-mailmailto:repositorio@ualg.ptrepositorio@ualg.ptISSNIsPartOf0044-8486URNIsPartOfAUT: DSI00813; LCA00739;DOIIsPartOfhttp://dx.doi.org/10.1016/j.aquaculture.2009.06.0202014-06-04T13:28:35Z20092014-05-30T10:04:41Z2009-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.1/4184http://purl.org/coar/access_right/c_abf2open accessTurbot MgpGene expressionSoft tissue accumulationHeartVertebraeBranchial arches9577303 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://sapientia.ualg.pt/bitstreams/7d0922e4-23e5-4ee1-8e76-861d99da4232/downloadAquaculture2943-4202211 |
| spellingShingle | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation Roberto, Vania Palma Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
| status | SINGLETON |
| subject.fl_str_mv | Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
| title | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| title_full | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| title_fullStr | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| title_full_unstemmed | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| title_short | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| title_sort | Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation |
| topic | Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
| topic_facet | Turbot Mgp Gene expression Soft tissue accumulation Heart Vertebrae Branchial arches |
| url | http://hdl.handle.net/10400.1/4184 |
| visible | 1 |