Publication
Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits
| Summary: | A new HPLC=DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobranc¸osa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step.The chromatographic separation was achieved using a Spherisorb ODS2 (25.060.46 cm; 5 mm, particle size) column.The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL=min. The detection limit values for phenolic compounds were between 0.04 and 4.32 mg=mL and the method was precise. As ageneral rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivars. |
|---|---|
| Main Authors: | Ferreira da Vinha, Ana |
| Other Authors: | Silva, Branca M.; Andrade, Paula B.; Seabra, Rosa M.; Pereira, José A.; Oliveira, M. Beatriz P.P. |
| Year: | 2002 |
| Country: | Portugal |
| Document type: | article |
| Access type: | restricted access |
| Associated institution: | Universidade Fernando Pessoa |
| Language: | English |
| Origin: | Repositório Institucional - Universidade Fernando Pessoa |
| _version_ | 1863850436112416768 |
|---|---|
| author | Ferreira da Vinha, Ana |
| author2 | Silva, Branca M. Andrade, Paula B. Seabra, Rosa M. Pereira, José A. Oliveira, M. Beatriz P.P. |
| author2_role | author author author author author |
| author_facet | Ferreira da Vinha, Ana Silva, Branca M. Andrade, Paula B. Seabra, Rosa M. Pereira, José A. Oliveira, M. Beatriz P.P. |
| author_role | author |
| contributor_name_str_mv | Repositório Institucional da Fernando Pessoa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Ferreira da Vinha, Ana\",\"Person.identifier.orcid\":\"0000-0002-6116-9593\"},{\"Person.name\":\"Silva, Branca M.\",\"Person.identifier.orcid\":\"0000-0002-5610-8024\"},{\"Person.name\":\"Andrade, Paula B.\"},{\"Person.name\":\"Seabra, Rosa M.\"},{\"Person.name\":\"Pereira, José A.\"},{\"Person.name\":\"Oliveira, M. Beatriz P.P.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Institucional da Fernando Pessoa |
| datacite.creators.creator.creatorName.fl_str_mv | Ferreira da Vinha, Ana Silva, Branca M. Andrade, Paula B. Seabra, Rosa M. Pereira, José A. Oliveira, M. Beatriz P.P. |
| datacite.date.Accepted.fl_str_mv | 2002-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-09-14T09:01:39Z |
| datacite.date.embargoed.fl_str_mv | 2020-09-14T09:01:39Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.titles.title.fl_str_mv | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| dc.contributor.none.fl_str_mv | Repositório Institucional da Fernando Pessoa |
| dc.creator.none.fl_str_mv | Ferreira da Vinha, Ana Silva, Branca M. Andrade, Paula B. Seabra, Rosa M. Pereira, José A. Oliveira, M. Beatriz P.P. |
| dc.date.Accepted.fl_str_mv | 2002-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-09-14T09:01:39Z |
| dc.date.embargoed.fl_str_mv | 2020-09-14T09:01:39Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10284/8961 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Taylor & Francis |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.title.fl_str_mv | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | A new HPLC=DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobranc¸osa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step.The chromatographic separation was achieved using a Spherisorb ODS2 (25.060.46 cm; 5 mm, particle size) column.The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL=min. The detection limit values for phenolic compounds were between 0.04 and 4.32 mg=mL and the method was precise. As ageneral rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivars. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bdigital.ufp.pt/bitstreams/4aeaa114-21b7-4f0a-ae9c-0b688d8fc1c4/download |
| id | ufp_ea35f4e68e4e17d59ff26d902253d42e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10284/8961 |
| instacron_str | ufp |
| institution | Universidade Fernando Pessoa |
| instname_str | Universidade Fernando Pessoa |
| language | eng |
| network_acronym_str | ufp |
| network_name_str | Repositório Institucional - Universidade Fernando Pessoa |
| oai_identifier_str | oai:bdigital.ufp.pt:10284/8961 |
| organization_str_mv | urn:organizationAcronym:ufp |
| person_str_mv | Ferreira da Vinha, Ana Ferreira da Vinha, Ana https://www.ciencia-id.pt/011A-FCBD-DC66 011A-FCBD-DC66 http://orcid.org/0000-0002-6116-9593 0000-0002-6116-9593 Silva, Branca M. Silva, Branca M. https://www.ciencia-id.pt/BD1F-B24D-89A9 BD1F-B24D-89A9 http://orcid.org/0000-0002-5610-8024 0000-0002-5610-8024 Andrade, Paula B. Seabra, Rosa M. Pereira, José A. Oliveira, M. Beatriz P.P. |
| publishDate | 2002 |
| publisher.none.fl_str_mv | Taylor & Francis |
| reponame_str | Repositório Institucional - Universidade Fernando Pessoa |
| repository_id_str | urn:repositoryAcronym:ufp |
| service_str_mv | urn:repositoryAcronym:ufp |
| spelling | engTaylor & Francispt_PTA new HPLC=DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobranc¸osa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step.The chromatographic separation was achieved using a Spherisorb ODS2 (25.060.46 cm; 5 mm, particle size) column.The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL=min. The detection limit values for phenolic compounds were between 0.04 and 4.32 mg=mL and the method was precise. As ageneral rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivars.application/pdfpt_PTDevelopment and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruitsPersonalFerreira da Vinha, AnaDSpacehttp://dspace.org/items/b09696c0-d5ea-4218-a5e4-63a3f6288d83DSpacehttp://dspace.org/items/b09696c0-d5ea-4218-a5e4-63a3f6288d83Mendes Ferreira da VinhaAna CristinaCiência IDhttps://www.ciencia-id.pt011A-FCBD-DC66ORCIDhttp://orcid.org0000-0002-6116-9593PersonalSilva, Branca M.DSpacehttp://dspace.org/items/26c68872-1849-4895-a48d-72ccffbc02dfDSpacehttp://dspace.org/items/26c68872-1849-4895-a48d-72ccffbc02dfSilvaBranca MariaCiência IDhttps://www.ciencia-id.ptBD1F-B24D-89A9ORCIDhttp://orcid.org0000-0002-5610-8024Scopus Author IDhttps://www.scopus.com7005615589Andrade, Paula B.Seabra, Rosa M.Pereira, José A.Oliveira, M. Beatriz P.P.HostingInstitutionOrganizationalRepositório Institucional da Fernando Pessoae-mailmailto:b-digital@ufp.ptb-digital@ufp.ptISSNIsPartOf1082-6076DOIIsPartOf10.1081/JLC-1001085462020-09-14T09:01:39Z20022002-01-01T00:00:00ZHandlehttp://hdl.handle.net/10284/8961http://purl.org/coar/access_right/c_16ecrestricted access333008 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bdigital.ufp.pt/bitstreams/4aeaa114-21b7-4f0a-ae9c-0b688d8fc1c4/downloadJournal of Liquid Chromatography and Related Technologies25151160 |
| spellingShingle | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits Ferreira da Vinha, Ana |
| title | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| title_full | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| title_fullStr | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| title_full_unstemmed | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| title_short | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| title_sort | Development and evaluation of an HPLC-DAD method for the phenolic compounds from olive fruits |
| url | http://hdl.handle.net/10284/8961 |
| visible | 1 |