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Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees

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Resumo:Historical factors (colonization scenarios, demographic oscillations) and contemporary processes (population connectivity, current population size) largely contribute to shaping species’ present-day genetic diversity and structure. In this study, we use a combination of mitochondrial and nuclear DNA markers to understand the role of Quaternary climatic oscillations and present-day gene flow dynamics in determining the genetic diversity and structure of the newt Calotriton asper (Al. Dugès, 1852), endemic to the Pyrenees. Mitochondrial DNA did not show a clear phylogeographic pattern and presented low levels of variation. In contrast, microsatellites revealed five major genetic lineages with admixture patterns at their boundaries. Approximate Bayesian computation analyses and linear models indicated that the five lineages likely underwent separate evolutionary histories and can be tracked back to distinct glacial refugia. Lineage differentiation started around the Last Glacial Maximum at three focal areas (western, central and eastern Pyrenees) and extended through the end of the Last Glacial Period in the central Pyrenees, where it led to the formation of two more lineages. Our data revealed no evidence of recent dispersal between lineages, whereas borders likely represent zones of secondary contact following expansion from multiple refugia. Finally, we did not find genetic evidence of sex-biased dispersal. This work highlights the importance of integrating past evolutionary processes and present-day gene flow and dispersal dynamics, together with multilocus approaches, to gain insights into what shaped the current genetic attributes of amphibians living in montane habitats.
Autores principais:Lucati, Federica
Outros Autores:Poignet, Manon; Miró, Alexandre; Trochet, Audrey; Aubret, Fabien; Barthe, Laurent; Bertrand, Romain; Buchaca, Teresa; Calvez, Olivier; Caner, Jenny; Darnet, Elodie; Denoël, Mathieu; Guillaume, Olivier; Le Chevalier, Hugo; Martínez‐Silvestre, Albert; Mossoll‐Torres, Marc; O’Brien, David; Osorio, Víctor; Pottier, Gilles; Richard, Murielle; Sabás, Ibor; Souchet, Jérémie; Tomàs, Jan; Ventura, Marc
Ano:2020
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa
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author Lucati, Federica
author2 Poignet, Manon
Miró, Alexandre
Trochet, Audrey
Aubret, Fabien
Barthe, Laurent
Bertrand, Romain
Buchaca, Teresa
Calvez, Olivier
Caner, Jenny
Darnet, Elodie
Denoël, Mathieu
Guillaume, Olivier
Le Chevalier, Hugo
Martínez‐Silvestre, Albert
Mossoll‐Torres, Marc
O’Brien, David
Osorio, Víctor
Pottier, Gilles
Richard, Murielle
Sabás, Ibor
Souchet, Jérémie
Tomàs, Jan
Ventura, Marc
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author_facet Lucati, Federica
Poignet, Manon
Miró, Alexandre
Trochet, Audrey
Aubret, Fabien
Barthe, Laurent
Bertrand, Romain
Buchaca, Teresa
Calvez, Olivier
Caner, Jenny
Darnet, Elodie
Denoël, Mathieu
Guillaume, Olivier
Le Chevalier, Hugo
Martínez‐Silvestre, Albert
Mossoll‐Torres, Marc
O’Brien, David
Osorio, Víctor
Pottier, Gilles
Richard, Murielle
Sabás, Ibor
Souchet, Jérémie
Tomàs, Jan
Ventura, Marc
author_role author
contributor_name_str_mv Repositório Científico de Acesso Aberto da ULisboa
country_str PT
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datacite.creators.creator.creatorName.fl_str_mv Lucati, Federica
Poignet, Manon
Miró, Alexandre
Trochet, Audrey
Aubret, Fabien
Barthe, Laurent
Bertrand, Romain
Buchaca, Teresa
Calvez, Olivier
Caner, Jenny
Darnet, Elodie
Denoël, Mathieu
Guillaume, Olivier
Le Chevalier, Hugo
Martínez‐Silvestre, Albert
Mossoll‐Torres, Marc
O’Brien, David
Osorio, Víctor
Pottier, Gilles
Richard, Murielle
Sabás, Ibor
Souchet, Jérémie
Tomàs, Jan
Ventura, Marc
datacite.date.Accepted.fl_str_mv 2020-08-01T00:00:00Z
datacite.date.available.fl_str_mv 2021-09-28T18:47:16Z
datacite.date.embargoed.fl_str_mv 2021-09-28T18:47:16Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.titles.title.fl_str_mv Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
dc.contributor.none.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
dc.creator.none.fl_str_mv Lucati, Federica
Poignet, Manon
Miró, Alexandre
Trochet, Audrey
Aubret, Fabien
Barthe, Laurent
Bertrand, Romain
Buchaca, Teresa
Calvez, Olivier
Caner, Jenny
Darnet, Elodie
Denoël, Mathieu
Guillaume, Olivier
Le Chevalier, Hugo
Martínez‐Silvestre, Albert
Mossoll‐Torres, Marc
O’Brien, David
Osorio, Víctor
Pottier, Gilles
Richard, Murielle
Sabás, Ibor
Souchet, Jérémie
Tomàs, Jan
Ventura, Marc
dc.date.Accepted.fl_str_mv 2020-08-01T00:00:00Z
dc.date.available.fl_str_mv 2021-09-28T18:47:16Z
dc.date.embargoed.fl_str_mv 2021-09-28T18:47:16Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10451/49669
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Wiley
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.title.fl_str_mv Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Historical factors (colonization scenarios, demographic oscillations) and contemporary processes (population connectivity, current population size) largely contribute to shaping species’ present-day genetic diversity and structure. In this study, we use a combination of mitochondrial and nuclear DNA markers to understand the role of Quaternary climatic oscillations and present-day gene flow dynamics in determining the genetic diversity and structure of the newt Calotriton asper (Al. Dugès, 1852), endemic to the Pyrenees. Mitochondrial DNA did not show a clear phylogeographic pattern and presented low levels of variation. In contrast, microsatellites revealed five major genetic lineages with admixture patterns at their boundaries. Approximate Bayesian computation analyses and linear models indicated that the five lineages likely underwent separate evolutionary histories and can be tracked back to distinct glacial refugia. Lineage differentiation started around the Last Glacial Maximum at three focal areas (western, central and eastern Pyrenees) and extended through the end of the Last Glacial Period in the central Pyrenees, where it led to the formation of two more lineages. Our data revealed no evidence of recent dispersal between lineages, whereas borders likely represent zones of secondary contact following expansion from multiple refugia. Finally, we did not find genetic evidence of sex-biased dispersal. This work highlights the importance of integrating past evolutionary processes and present-day gene flow and dispersal dynamics, together with multilocus approaches, to gain insights into what shaped the current genetic attributes of amphibians living in montane habitats.
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institution Universidade de Lisboa
instname_str Universidade de Lisboa
language eng
network_acronym_str ul
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organization_str_mv urn:organizationAcronym:ul
person_str_mv Lucati, Federica
Lucati, Federica
http://orcid.org/0000-0001-9925-7137
0000-0001-9925-7137
Poignet, Manon
Miró, Alexandre
Trochet, Audrey
Aubret, Fabien
Barthe, Laurent
Bertrand, Romain
Buchaca, Teresa
Calvez, Olivier
Caner, Jenny
Darnet, Elodie
Denoël, Mathieu
Guillaume, Olivier
Le Chevalier, Hugo
Martínez‐Silvestre, Albert
Mossoll‐Torres, Marc
O’Brien, David
Osorio, Víctor
Pottier, Gilles
Richard, Murielle
Sabás, Ibor
Souchet, Jérémie
Tomàs, Jan
Ventura, Marc
publishDate 2020
publisher.none.fl_str_mv Wiley
reponame_str Repositório da Universidade de Lisboa
repository_id_str urn:repositoryAcronym:ul
service_str_mv urn:repositoryAcronym:ul
spelling engWileypt_PTHistorical factors (colonization scenarios, demographic oscillations) and contemporary processes (population connectivity, current population size) largely contribute to shaping species’ present-day genetic diversity and structure. In this study, we use a combination of mitochondrial and nuclear DNA markers to understand the role of Quaternary climatic oscillations and present-day gene flow dynamics in determining the genetic diversity and structure of the newt Calotriton asper (Al. Dugès, 1852), endemic to the Pyrenees. Mitochondrial DNA did not show a clear phylogeographic pattern and presented low levels of variation. In contrast, microsatellites revealed five major genetic lineages with admixture patterns at their boundaries. Approximate Bayesian computation analyses and linear models indicated that the five lineages likely underwent separate evolutionary histories and can be tracked back to distinct glacial refugia. Lineage differentiation started around the Last Glacial Maximum at three focal areas (western, central and eastern Pyrenees) and extended through the end of the Last Glacial Period in the central Pyrenees, where it led to the formation of two more lineages. Our data revealed no evidence of recent dispersal between lineages, whereas borders likely represent zones of secondary contact following expansion from multiple refugia. Finally, we did not find genetic evidence of sex-biased dispersal. This work highlights the importance of integrating past evolutionary processes and present-day gene flow and dispersal dynamics, together with multilocus approaches, to gain insights into what shaped the current genetic attributes of amphibians living in montane habitats.application/pdfpt_PTMultiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the PyreneesPersonalLucati, FedericaDSpacehttp://dspace.org/items/ad31bc71-51a8-45a6-a262-7f49f20a2af9DSpacehttp://dspace.org/items/ad31bc71-51a8-45a6-a262-7f49f20a2af9LucatiFedericaORCIDhttp://orcid.org0000-0001-9925-7137Scopus Author IDhttps://www.scopus.com57192820646Poignet, ManonMiró, AlexandreTrochet, AudreyAubret, FabienBarthe, LaurentBertrand, RomainBuchaca, TeresaCalvez, OlivierCaner, JennyDarnet, ElodieDenoël, MathieuGuillaume, OlivierLe Chevalier, HugoMartínez‐Silvestre, AlbertMossoll‐Torres, MarcO’Brien, DavidOsorio, VíctorPottier, GillesRichard, MurielleSabás, IborSouchet, JérémieTomàs, JanVentura, MarcHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.1111/mec.155212021-09-28T18:47:16Z2020-082020-08-01T00:00:00ZHandlehttp://hdl.handle.net/10451/49669http://purl.org/coar/access_right/c_abf2open access2161111 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/63584d47-8825-4989-a58f-a1a7730b0013/downloadMolecular Ecology291529042921
spellingShingle Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
Lucati, Federica
status SINGLETON
title Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
title_full Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
title_fullStr Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
title_full_unstemmed Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
title_short Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
title_sort Multiple glacial refugia and contemporary dispersal shape the genetic structure of an endemic amphibian from the Pyrenees
url http://hdl.handle.net/10451/49669
visible 1