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Characterization of Portuguese miso by solid state fermentation of Portuguese pulses

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Resumo:Fermentation is one of the oldest conservation techniques, resulting in products with a range of health benefits. The combination of an increase in demand for plant-based products, sustainability concerns and fermented products coupled with miso gain of attraction, due to its flavor and health benefits, assures the potential of this product. This work integrates a project that encompasses the development of new fermented products using Portuguese pulses (chickpea and lupin) as an alternative to typical soybean with the aim of increase sustainability while maintaining controlled traditional fermenting processes and standardized processing. With this objective, miso from chickpea and lupin were produced with 3% and 12% NaCl. Fermentation process was evaluated through 90 days of fermentation and physic-chemical characterization of the final product was performed. Aspects related to health benefits were also determined. Throughout fermentation, an increase in acidity resulting from the metabolic activity of the microorganisms and a decrease in the number of yeast and bacteria UFCs was registered, with lower values for samples with higher salt concentration. Reducing sugars and organic acid content increase. The nutritional value of miso, total phenolic and the antioxidant content increase after 90 days with higher values for lupin miso. Firmness and adhesiveness show a decrease. Besides, while chickpeas demonstrate an internal structure evolution in agreement with the texture results, lupins have an opposite evolution. A darkening of the miso was observed due to Maillard reactions and enzymatic oxidation. Results obtained showed that properties regarding microbiota evolution, physicochemical parameters, nutritional value, texture, internal structure, and color were influenced by the product's formulation and processing, being evident the influence of salt and pulse. Microorganisms present in the samples and koji enzymes are responsible for the fermentation process, contributing to an increase in conservation time, development of its characteristic flavor and improvement of nutritional value.
Autores principais:Parente, Beatriz Silva dos Santos Casanova
Assunto:miso fermentation salt chickpea lupin fermentação sal grão tremoço
Ano:2023
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso restrito
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa
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author Parente, Beatriz Silva dos Santos Casanova
author_facet Parente, Beatriz Silva dos Santos Casanova
author_role author
contributor_name_str_mv Prista, Catarina Geoffroy
Raymundo, Anabela Moreira
Repositório Científico de Acesso Aberto da ULisboa
country_str PT
creators_json_txt [{\"Person.name\":\"Parente, Beatriz Silva dos Santos Casanova\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Prista, Catarina Geoffroy
Raymundo, Anabela Moreira
Repositório Científico de Acesso Aberto da ULisboa
datacite.creators.creator.creatorName.fl_str_mv Parente, Beatriz Silva dos Santos Casanova
datacite.date.Accepted.fl_str_mv 2023-12-05T00:00:00Z
datacite.date.available.fl_str_mv 2025-05-31T00:30:55Z
datacite.date.embargoed.fl_str_mv 2025-05-31T00:30:55Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv miso
fermentation
salt
chickpea
lupin
fermentação
sal
grão
tremoço
datacite.titles.title.fl_str_mv Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
dc.contributor.none.fl_str_mv Prista, Catarina Geoffroy
Raymundo, Anabela Moreira
Repositório Científico de Acesso Aberto da ULisboa
dc.creator.none.fl_str_mv Parente, Beatriz Silva dos Santos Casanova
dc.date.Accepted.fl_str_mv 2023-12-05T00:00:00Z
dc.date.available.fl_str_mv 2025-05-31T00:30:55Z
dc.date.embargoed.fl_str_mv 2025-05-31T00:30:55Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10400.5/30926
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Instituto Superior de Agronomia, Universidade de Lisboa
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv miso
fermentation
salt
chickpea
lupin
fermentação
sal
grão
tremoço
dc.title.fl_str_mv Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_bdcc
description Fermentation is one of the oldest conservation techniques, resulting in products with a range of health benefits. The combination of an increase in demand for plant-based products, sustainability concerns and fermented products coupled with miso gain of attraction, due to its flavor and health benefits, assures the potential of this product. This work integrates a project that encompasses the development of new fermented products using Portuguese pulses (chickpea and lupin) as an alternative to typical soybean with the aim of increase sustainability while maintaining controlled traditional fermenting processes and standardized processing. With this objective, miso from chickpea and lupin were produced with 3% and 12% NaCl. Fermentation process was evaluated through 90 days of fermentation and physic-chemical characterization of the final product was performed. Aspects related to health benefits were also determined. Throughout fermentation, an increase in acidity resulting from the metabolic activity of the microorganisms and a decrease in the number of yeast and bacteria UFCs was registered, with lower values for samples with higher salt concentration. Reducing sugars and organic acid content increase. The nutritional value of miso, total phenolic and the antioxidant content increase after 90 days with higher values for lupin miso. Firmness and adhesiveness show a decrease. Besides, while chickpeas demonstrate an internal structure evolution in agreement with the texture results, lupins have an opposite evolution. A darkening of the miso was observed due to Maillard reactions and enzymatic oxidation. Results obtained showed that properties regarding microbiota evolution, physicochemical parameters, nutritional value, texture, internal structure, and color were influenced by the product's formulation and processing, being evident the influence of salt and pulse. Microorganisms present in the samples and koji enzymes are responsible for the fermentation process, contributing to an increase in conservation time, development of its characteristic flavor and improvement of nutritional value.
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identifier.url.fl_str_mv http://hdl.handle.net/10400.5/30926
inst_facet_str urn:organizationAcronym:ul{{{_:::_}}}Universidade de Lisboa
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institution Universidade de Lisboa
instname_str Universidade de Lisboa
language eng
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network_name_str Repositório da Universidade de Lisboa
oai_identifier_str oai:repositorio.ulisboa.pt:10400.5/30926
organization_str_mv urn:organizationAcronym:ul
person_str_mv Parente, Beatriz Silva dos Santos Casanova
publishDate 2023
publisher.none.fl_str_mv Instituto Superior de Agronomia, Universidade de Lisboa
repo_facet_str urn:repositoryAcronym:ul{{{_:::_}}}Repositório da Universidade de Lisboa
reponame_str Repositório da Universidade de Lisboa
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spelling engInstituto Superior de Agronomia, Universidade de Lisboapt_PTFermentation is one of the oldest conservation techniques, resulting in products with a range of health benefits. The combination of an increase in demand for plant-based products, sustainability concerns and fermented products coupled with miso gain of attraction, due to its flavor and health benefits, assures the potential of this product. This work integrates a project that encompasses the development of new fermented products using Portuguese pulses (chickpea and lupin) as an alternative to typical soybean with the aim of increase sustainability while maintaining controlled traditional fermenting processes and standardized processing. With this objective, miso from chickpea and lupin were produced with 3% and 12% NaCl. Fermentation process was evaluated through 90 days of fermentation and physic-chemical characterization of the final product was performed. Aspects related to health benefits were also determined. Throughout fermentation, an increase in acidity resulting from the metabolic activity of the microorganisms and a decrease in the number of yeast and bacteria UFCs was registered, with lower values for samples with higher salt concentration. Reducing sugars and organic acid content increase. The nutritional value of miso, total phenolic and the antioxidant content increase after 90 days with higher values for lupin miso. Firmness and adhesiveness show a decrease. Besides, while chickpeas demonstrate an internal structure evolution in agreement with the texture results, lupins have an opposite evolution. A darkening of the miso was observed due to Maillard reactions and enzymatic oxidation. Results obtained showed that properties regarding microbiota evolution, physicochemical parameters, nutritional value, texture, internal structure, and color were influenced by the product's formulation and processing, being evident the influence of salt and pulse. Microorganisms present in the samples and koji enzymes are responsible for the fermentation process, contributing to an increase in conservation time, development of its characteristic flavor and improvement of nutritional value.application/pdfpt_PTCharacterization of Portuguese miso by solid state fermentation of Portuguese pulsesParente, Beatriz Silva dos Santos CasanovaPrista, Catarina GeoffroyRaymundo, Anabela MoreiraHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@ulisboa.ptrepositorio@ulisboa.ptURNurn:tid:2035697252025-05-31T00:30:55Z2023-12-052023-12-05T00:00:00ZHandlehttp://hdl.handle.net/10400.5/30926http://purl.org/coar/access_right/c_16ecrestricted accessmisofermentationsaltchickpealupinfermentaçãosalgrãotremoço1476654 bytesliteraturehttp://purl.org/coar/resource_type/c_bdccmaster thesishttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/a871d54e-8295-437e-9f45-b23223c65182/download
spellingShingle Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
Parente, Beatriz Silva dos Santos Casanova
miso
fermentation
salt
chickpea
lupin
fermentação
sal
grão
tremoço
status SINGLETON
subject.fl_str_mv miso
fermentation
salt
chickpea
lupin
fermentação
sal
grão
tremoço
title Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
title_full Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
title_fullStr Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
title_full_unstemmed Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
title_short Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
title_sort Characterization of Portuguese miso by solid state fermentation of Portuguese pulses
topic miso
fermentation
salt
chickpea
lupin
fermentação
sal
grão
tremoço
topic_facet miso
fermentation
salt
chickpea
lupin
fermentação
sal
grão
tremoço
url http://hdl.handle.net/10400.5/30926
visible 1