Publicação
Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation
| Resumo: | To gain new insights about the genetic networks controlling hair cell (HC) development, we previously developed a direct genetic programming strategy to generate an inexhaustible supply of HC-like cells (induced HCs, iHCs) in vitro, starting from mouse embryonic stem cells (ESC). We found that combined activity of three transcription factors, Gfi1, Pou4f3, and Atoh1, can program ESC-derived progenitors towards HC fate with efficiencies of 55%-80%. These iHCs express several HC markers and exhibit polarized structures that are highly reminiscent of the mechanosensitive hair bundles, with many microvilli-like stereocilia. Here, we describe the experimental design, methodology, and data validation for the microarray analysis used to characterize the transcriptome profile of iHCs at different stages of their differentiation. This approach based on FACS sorting and microarray analysis revealed a highly similar iHC transcriptome to that of endogenous HCs in vivo. The data obtained in this study is available in the Gene Expression Omnibus (GEO) database (accession number GSE60352). |
|---|---|
| Autores principais: | Costa, Aida |
| Outros Autores: | Henrique, Domingos |
| Assunto: | Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1865920781498712064 |
|---|---|
| author | Costa, Aida |
| author2 | Henrique, Domingos |
| author2_role | author |
| author_facet | Costa, Aida Costa, Aida Henrique, Domingos Henrique, Domingos |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Costa, Aida\",\"Person.identifier.orcid\":\"0000-0002-5314-3895\"},{\"Person.name\":\"Henrique, Domingos\",\"Person.identifier.orcid\":\"0000-0001-8869-1894\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Costa, Aida Henrique, Domingos |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-02-11T16:23:13Z |
| datacite.date.embargoed.fl_str_mv | 2022-02-11T16:23:13Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling |
| datacite.titles.title.fl_str_mv | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Costa, Aida Henrique, Domingos |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-02-11T16:23:13Z |
| dc.date.embargoed.fl_str_mv | 2022-02-11T16:23:13Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/51235 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling |
| dc.title.fl_str_mv | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | To gain new insights about the genetic networks controlling hair cell (HC) development, we previously developed a direct genetic programming strategy to generate an inexhaustible supply of HC-like cells (induced HCs, iHCs) in vitro, starting from mouse embryonic stem cells (ESC). We found that combined activity of three transcription factors, Gfi1, Pou4f3, and Atoh1, can program ESC-derived progenitors towards HC fate with efficiencies of 55%-80%. These iHCs express several HC markers and exhibit polarized structures that are highly reminiscent of the mechanosensitive hair bundles, with many microvilli-like stereocilia. Here, we describe the experimental design, methodology, and data validation for the microarray analysis used to characterize the transcriptome profile of iHCs at different stages of their differentiation. This approach based on FACS sorting and microarray analysis revealed a highly similar iHC transcriptome to that of endogenous HCs in vivo. The data obtained in this study is available in the Gene Expression Omnibus (GEO) database (accession number GSE60352). |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/65b63545-b94b-477d-b046-e497e1286a85/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 3599-PPCDT |
| id | ul_2cc0d64d2bc6697160fed7224fc6fe8d |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/51235 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/51235 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Costa, Aida Costa, Aida http://orcid.org/0000-0002-5314-3895 0000-0002-5314-3895 Henrique, Domingos Henrique, Domingos https://www.ciencia-id.pt/C111-5D28-EC65 C111-5D28-EC65 http://orcid.org/0000-0001-8869-1894 0000-0001-8869-1894 |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engElsevierpt_PTTo gain new insights about the genetic networks controlling hair cell (HC) development, we previously developed a direct genetic programming strategy to generate an inexhaustible supply of HC-like cells (induced HCs, iHCs) in vitro, starting from mouse embryonic stem cells (ESC). We found that combined activity of three transcription factors, Gfi1, Pou4f3, and Atoh1, can program ESC-derived progenitors towards HC fate with efficiencies of 55%-80%. These iHCs express several HC markers and exhibit polarized structures that are highly reminiscent of the mechanosensitive hair bundles, with many microvilli-like stereocilia. Here, we describe the experimental design, methodology, and data validation for the microarray analysis used to characterize the transcriptome profile of iHCs at different stages of their differentiation. This approach based on FACS sorting and microarray analysis revealed a highly similar iHC transcriptome to that of endogenous HCs in vivo. The data obtained in this study is available in the Gene Expression Omnibus (GEO) database (accession number GSE60352).application/pdfpt_PTTranscriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiationPersonalCosta, AidaDSpacehttp://dspace.org/items/1d570b0e-700f-4522-8897-03a8002debcaDSpacehttp://dspace.org/items/1d570b0e-700f-4522-8897-03a8002debcaCostaAidaORCIDhttp://orcid.org0000-0002-5314-3895PersonalHenrique, DomingosDSpacehttp://dspace.org/items/d66f1f29-0686-4c76-97b0-4f350eafdcb8DSpacehttp://dspace.org/items/d66f1f29-0686-4c76-97b0-4f350eafdcb8Pinto HenriqueDomingos ManuelCiência IDhttps://www.ciencia-id.ptC111-5D28-EC65ORCIDhttp://orcid.org0000-0001-8869-1894HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf2213-5960DOIIsPartOf10.1016/j.gdata.2015.08.0172022-02-11T16:23:13Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/51235http://purl.org/coar/access_right/c_abf2open accessCell type programmingHair cellsIn vitro differentiationMouse embryonic stem cellsTranscriptome profiling721456 bytesFundação para a Ciência e a TecnologiaStem cell based therapy for inner ear hair cell regeneration3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaDEVELOPMENT OF A CELL-REPLACEMENT THERAPY TO REGENERATE SENSORY HAIR CELLS IN THE MAMMALIAN INNER EARCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2015http://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/65b63545-b94b-477d-b046-e497e1286a85/downloadGenomics Data67780 |
| spellingShingle | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation Costa, Aida Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling Costa, Aida Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling |
| status | SINGLETON |
| subject.fl_str_mv | Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling |
| title | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| title_full | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| title_fullStr | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| title_full_unstemmed | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| title_short | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| title_sort | Transcriptome profiling of induced hair cells (iHCs) generated by combined expression of Gfi1, Pou4f3 and Atoh1 during embryonic stem cell differentiation |
| topic | Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling |
| topic_facet | Cell type programming Hair cells In vitro differentiation Mouse embryonic stem cells Transcriptome profiling |
| url | http://hdl.handle.net/10451/51235 |
| visible | 1 |