Publicação
Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity
| Resumo: | The most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presented |
|---|---|
| Autores principais: | Santos, Carmen |
| Outros Autores: | Duarte, Sofia; Tedesco, Sara; Fevereiro, Pedro; Costa, Rita L. |
| Assunto: | Castanea Phytophthora cinnamomi ink disease plant biotic interactions digital PCR |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866811284684537856 |
|---|---|
| author | Santos, Carmen |
| author2 | Duarte, Sofia Tedesco, Sara Fevereiro, Pedro Costa, Rita L. |
| author2_role | author author author author |
| author_facet | Santos, Carmen Duarte, Sofia Tedesco, Sara Fevereiro, Pedro Costa, Rita L. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Santos, Carmen\"},{\"Person.name\":\"Duarte, Sofia\"},{\"Person.name\":\"Tedesco, Sara\"},{\"Person.name\":\"Fevereiro, Pedro\"},{\"Person.name\":\"Costa, Rita L.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Santos, Carmen Duarte, Sofia Tedesco, Sara Fevereiro, Pedro Costa, Rita L. |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2017-06-02T13:20:06Z |
| datacite.date.embargoed.fl_str_mv | 2017-06-02T13:20:06Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Castanea Phytophthora cinnamomi ink disease plant biotic interactions digital PCR |
| datacite.titles.title.fl_str_mv | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Santos, Carmen Duarte, Sofia Tedesco, Sara Fevereiro, Pedro Costa, Rita L. |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2017-06-02T13:20:06Z |
| dc.date.embargoed.fl_str_mv | 2017-06-02T13:20:06Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/13711 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | FPLS |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Castanea Phytophthora cinnamomi ink disease plant biotic interactions digital PCR |
| dc.title.fl_str_mv | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presented |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/5716b886-3726-4bfb-b511-493adc24efd8/download |
| id | ul_346ed99267b27f80bb2bd18cd37b6021 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/13711 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/13711 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Santos, Carmen Duarte, Sofia Tedesco, Sara Fevereiro, Pedro Costa, Rita L. |
| publishDate | 2017 |
| publisher.none.fl_str_mv | FPLS |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engFPLSpt_PTThe most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presentedapplication/pdfpt_PTExpression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunitySantos, CarmenDuarte, SofiaTedesco, SaraFevereiro, PedroCosta, Rita L.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.3389/fpls.2017.005152017-06-02T13:20:06Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/13711http://purl.org/coar/access_right/c_abf2open accessCastaneaPhytophthora cinnamomiink diseaseplant biotic interactionsdigital PCR1231389 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/5716b886-3726-4bfb-b511-493adc24efd8/downloadFrontiers in Plant Science |
| spellingShingle | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity Santos, Carmen Castanea Phytophthora cinnamomi ink disease plant biotic interactions digital PCR |
| status | SINGLETON |
| subject.fl_str_mv | Castanea Phytophthora cinnamomi ink disease plant biotic interactions digital PCR |
| title | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| title_full | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| title_fullStr | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| title_full_unstemmed | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| title_short | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| title_sort | Expression profiling of Castanea genes during resistant and susceptible interactions with the Oomycete pathogen Phytophthora cinnamomi reveal possible mechanisms of immunity |
| topic | Castanea Phytophthora cinnamomi ink disease plant biotic interactions digital PCR |
| topic_facet | Castanea Phytophthora cinnamomi ink disease plant biotic interactions digital PCR |
| url | http://hdl.handle.net/10400.5/13711 |
| visible | 1 |