Publicação
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
| Resumo: | Aims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures. |
|---|---|
| Autores principais: | Bravo-Ferrada, B. M. |
| Outros Autores: | Gonçalves, S.; Semorile, L.; Santos, N. C.; Tymczyszyn, E. E.; Hollmann, A. |
| Assunto: | Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1865920783885271040 |
|---|---|
| author | Bravo-Ferrada, B. M. |
| author2 | Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
| author2_role | author author author author author |
| author_facet | Bravo-Ferrada, B. M. Bravo-Ferrada, B. M. Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Bravo-Ferrada, B. M.\"},{\"Person.name\":\"Gonçalves, S.\"},{\"Person.name\":\"Semorile, L.\"},{\"Person.name\":\"Santos, N. C.\"},{\"Person.name\":\"Tymczyszyn, E. E.\"},{\"Person.name\":\"Hollmann, A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Bravo-Ferrada, B. M. Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-05-04T15:32:41Z |
| datacite.date.embargoed.fl_str_mv | 2016-05-04T15:32:41Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
| datacite.titles.title.fl_str_mv | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Bravo-Ferrada, B. M. Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-05-04T15:32:41Z |
| dc.date.embargoed.fl_str_mv | 2016-05-04T15:32:41Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/23602 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
| dc.title.fl_str_mv | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Aims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/1df82afe-7f26-41dc-9450-8bd58762c4cb/download |
| id | ul_34e7f1f5ae1959cd0f8d77491e5e893e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/23602 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/23602 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Bravo-Ferrada, B. M. Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Wiley |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engWileypt_PTAims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.application/pdfpt_PTStudy of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydrationBravo-Ferrada, B. M.Gonçalves, S.Semorile, L.Santos, N. C.Tymczyszyn, E. E.Hollmann, A.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1364-5072DOIIsPartOf10.1111/jam.127962016-05-04T15:32:41Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/23602http://purl.org/coar/access_right/c_16ecrestricted accessAtomic force microscopyFreeze-dryingLactobacillus plantarumWineZeta potential716443 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/1df82afe-7f26-41dc-9450-8bd58762c4cb/downloadJournal of Applied Microbiology |
| spellingShingle | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration Bravo-Ferrada, B. M. Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential Bravo-Ferrada, B. M. Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
| status | SINGLETON |
| subject.fl_str_mv | Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
| title | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| title_full | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| title_fullStr | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| title_full_unstemmed | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| title_short | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| title_sort | Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
| topic | Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
| topic_facet | Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
| url | http://hdl.handle.net/10451/23602 |
| visible | 1 |