Publicação
Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia
| Resumo: | Recent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control aliquots) and presence of fibrinogen at different degrees of band 3 phosphorylation, and the erythrocyte deformability was determined. The present study shows that in the presence of higher fibrinogen concentrations, similar to those found in inflammatory conditions, erythrocyte deformability is increased only when band 3 is dephosphorylated by the presence of syk inhibitor and at low shear stress. On the contrary, no changes were verified in the presence of fibrinogen when band 3 is allowed to be phosphorylated by inhibiting the phosphotyrosine phosphatase enzyme activity with calpeptin. We also observed that the presence of fibrinogen at higher concentration does not induce changes in erythrocyte deformability in the absence of modulators of the band 3 phosphorylation degree. However, the mechanisms by which fibrinogen signalling modulates erythrocyte function remain to be clarified and are currently under study. |
|---|---|
| Autores principais: | Almeida, J. P. Lopes de |
| Outros Autores: | Freitas-Santos, T.; Saldanha, C. |
| Assunto: | Band 3 protein Erythrocyte Deformability Fibrinogen |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810500983029760 |
|---|---|
| author | Almeida, J. P. Lopes de |
| author2 | Freitas-Santos, T. Saldanha, C. |
| author2_role | author author |
| author_facet | Almeida, J. P. Lopes de Freitas-Santos, T. Saldanha, C. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Almeida, J. P. Lopes de\"},{\"Person.name\":\"Freitas-Santos, T.\"},{\"Person.name\":\"Saldanha, C.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Almeida, J. P. Lopes de Freitas-Santos, T. Saldanha, C. |
| datacite.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-03-05T10:54:34Z |
| datacite.date.embargoed.fl_str_mv | 2014-03-05T10:54:34Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Band 3 protein Erythrocyte Deformability Fibrinogen |
| datacite.titles.title.fl_str_mv | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Almeida, J. P. Lopes de Freitas-Santos, T. Saldanha, C. |
| dc.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-03-05T10:54:34Z |
| dc.date.embargoed.fl_str_mv | 2014-03-05T10:54:34Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://dx.doi.org/10.3233/CH-2010-1433 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | IOS Press |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Band 3 protein Erythrocyte Deformability Fibrinogen |
| dc.title.fl_str_mv | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Recent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control aliquots) and presence of fibrinogen at different degrees of band 3 phosphorylation, and the erythrocyte deformability was determined. The present study shows that in the presence of higher fibrinogen concentrations, similar to those found in inflammatory conditions, erythrocyte deformability is increased only when band 3 is dephosphorylated by the presence of syk inhibitor and at low shear stress. On the contrary, no changes were verified in the presence of fibrinogen when band 3 is allowed to be phosphorylated by inhibiting the phosphotyrosine phosphatase enzyme activity with calpeptin. We also observed that the presence of fibrinogen at higher concentration does not induce changes in erythrocyte deformability in the absence of modulators of the band 3 phosphorylation degree. However, the mechanisms by which fibrinogen signalling modulates erythrocyte function remain to be clarified and are currently under study. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/0c0cc022-3b98-4c95-a286-3e789d651239/download |
| id | ul_362bbc6411dedf62a2de8d2bfa28bf09 |
| identifier.doi.fl_str_mv | http://dx.doi.org/10.3233/CH-2010-1433 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/10677 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Almeida, J. P. Lopes de Freitas-Santos, T. Saldanha, C. |
| publishDate | 2012 |
| publisher.none.fl_str_mv | IOS Press |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engIOS PressengRecent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control aliquots) and presence of fibrinogen at different degrees of band 3 phosphorylation, and the erythrocyte deformability was determined. The present study shows that in the presence of higher fibrinogen concentrations, similar to those found in inflammatory conditions, erythrocyte deformability is increased only when band 3 is dephosphorylated by the presence of syk inhibitor and at low shear stress. On the contrary, no changes were verified in the presence of fibrinogen when band 3 is allowed to be phosphorylated by inhibiting the phosphotyrosine phosphatase enzyme activity with calpeptin. We also observed that the presence of fibrinogen at higher concentration does not induce changes in erythrocyte deformability in the absence of modulators of the band 3 phosphorylation degree. However, the mechanisms by which fibrinogen signalling modulates erythrocyte function remain to be clarified and are currently under study.application/pdfengErythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemiaAlmeida, J. P. Lopes deFreitas-Santos, T.Saldanha, C.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptHandlehttp://hdl.handle.net/10451/10677ISSNIsPartOf1386-02912014-03-05T10:54:34Z20122012-01-01T00:00:00ZDOIhttp://dx.doi.org/10.3233/CH-2010-1433http://purl.org/coar/access_right/c_16ecrestricted accessBand 3 proteinErythrocyteDeformabilityFibrinogen57892 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/0c0cc022-3b98-4c95-a286-3e789d651239/downloadClinical Hemorheology and Microcirculation50213219 |
| spellingShingle | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia Almeida, J. P. Lopes de Band 3 protein Erythrocyte Deformability Fibrinogen |
| status | SINGLETON |
| subject.fl_str_mv | Band 3 protein Erythrocyte Deformability Fibrinogen |
| title | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| title_full | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| title_fullStr | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| title_full_unstemmed | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| title_short | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| title_sort | Erythrocyte deformability dependence on band 3 protein in an in-vitro model of hyperfibrinogenemia |
| topic | Band 3 protein Erythrocyte Deformability Fibrinogen |
| topic_facet | Band 3 protein Erythrocyte Deformability Fibrinogen |
| url | http://dx.doi.org/10.3233/CH-2010-1433 |
| visible | 1 |