Publicação
Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
| Resumo: | Motivation: The need for more effective and safer pharmaceuticals is a persistent quest. Microbial adaptations create the need to permanently develop new antimicrobials, for instance. Similarly, intracellular delivery of drugs is still a challenge and translocation of membranes for drug delivery is an area of intense research. Peptides can be used both as antimicrobial drug leads and drug carrier systems for intracellular delivery. Multifunctional proteins are abundant in viruses but, surprisingly, have never been thoroughly screened for bioactive peptide sequences. Results: Using the AMPA and CellPPD online tools we have evaluated the propensity of viral proteins to comprise antimicrobial (AMP) or cell-penetrating peptides (CPP). Capsid proteins from both enveloped and non-enveloped viruses, and membrane and envelope proteins from enveloped viruses, in a total of 272 proteins from 133 viruses, were screened to detect the presence of potential AMP and CPP sequences. A pool of 2444 and 426 CPP and AMP sequences, respectively, were discovered. The capsids of flaviviruses are the best sources of these peptides reaching more than 80% of CPP sequence coverage per protein. Selected sequences were tested experimentally and validated the results. Overall, this study reveals that viruses form a natural multivalent biotechnological platform still underexplored in drug discovery and the heterogeneous abundance of CPP/AMP sequences among viral families opens new avenues in viral biology research. |
|---|---|
| Autores principais: | Freire, João Miguel |
| Outros Autores: | Dias, Susana Almeida; Flores, Luís; Veiga, Ana Salomé; Castanho, Miguel A. R. B. |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810234536722432 |
|---|---|
| author | Freire, João Miguel |
| author2 | Dias, Susana Almeida Flores, Luís Veiga, Ana Salomé Castanho, Miguel A. R. B. |
| author2_role | author author author author |
| author_facet | Freire, João Miguel Dias, Susana Almeida Flores, Luís Veiga, Ana Salomé Castanho, Miguel A. R. B. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Freire, João Miguel\"},{\"Person.name\":\"Dias, Susana Almeida\"},{\"Person.name\":\"Flores, Luís\"},{\"Person.name\":\"Veiga, Ana Salomé\"},{\"Person.name\":\"Castanho, Miguel A. R. B.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Freire, João Miguel Dias, Susana Almeida Flores, Luís Veiga, Ana Salomé Castanho, Miguel A. R. B. |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2016-03-18T15:01:49Z |
| datacite.date.embargoed.fl_str_mv | 2016-03-18T15:01:49Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.titles.title.fl_str_mv | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Freire, João Miguel Dias, Susana Almeida Flores, Luís Veiga, Ana Salomé Castanho, Miguel A. R. B. |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2016-03-18T15:01:49Z |
| dc.date.embargoed.fl_str_mv | 2016-03-18T15:01:49Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/23081 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Oxford University Press |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.title.fl_str_mv | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Motivation: The need for more effective and safer pharmaceuticals is a persistent quest. Microbial adaptations create the need to permanently develop new antimicrobials, for instance. Similarly, intracellular delivery of drugs is still a challenge and translocation of membranes for drug delivery is an area of intense research. Peptides can be used both as antimicrobial drug leads and drug carrier systems for intracellular delivery. Multifunctional proteins are abundant in viruses but, surprisingly, have never been thoroughly screened for bioactive peptide sequences. Results: Using the AMPA and CellPPD online tools we have evaluated the propensity of viral proteins to comprise antimicrobial (AMP) or cell-penetrating peptides (CPP). Capsid proteins from both enveloped and non-enveloped viruses, and membrane and envelope proteins from enveloped viruses, in a total of 272 proteins from 133 viruses, were screened to detect the presence of potential AMP and CPP sequences. A pool of 2444 and 426 CPP and AMP sequences, respectively, were discovered. The capsids of flaviviruses are the best sources of these peptides reaching more than 80% of CPP sequence coverage per protein. Selected sequences were tested experimentally and validated the results. Overall, this study reveals that viruses form a natural multivalent biotechnological platform still underexplored in drug discovery and the heterogeneous abundance of CPP/AMP sequences among viral families opens new avenues in viral biology research. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/b14cba1a-6af9-4ffe-85cd-c74bc78ef930/download |
| id | ul_39c5d38e41b82fa9cd4bf833eaa475bd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/23081 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/23081 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Freire, João Miguel Dias, Susana Almeida Flores, Luís Veiga, Ana Salomé Castanho, Miguel A. R. B. |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Oxford University Press |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engOxford University Presspt_PTMotivation: The need for more effective and safer pharmaceuticals is a persistent quest. Microbial adaptations create the need to permanently develop new antimicrobials, for instance. Similarly, intracellular delivery of drugs is still a challenge and translocation of membranes for drug delivery is an area of intense research. Peptides can be used both as antimicrobial drug leads and drug carrier systems for intracellular delivery. Multifunctional proteins are abundant in viruses but, surprisingly, have never been thoroughly screened for bioactive peptide sequences. Results: Using the AMPA and CellPPD online tools we have evaluated the propensity of viral proteins to comprise antimicrobial (AMP) or cell-penetrating peptides (CPP). Capsid proteins from both enveloped and non-enveloped viruses, and membrane and envelope proteins from enveloped viruses, in a total of 272 proteins from 133 viruses, were screened to detect the presence of potential AMP and CPP sequences. A pool of 2444 and 426 CPP and AMP sequences, respectively, were discovered. The capsids of flaviviruses are the best sources of these peptides reaching more than 80% of CPP sequence coverage per protein. Selected sequences were tested experimentally and validated the results. Overall, this study reveals that viruses form a natural multivalent biotechnological platform still underexplored in drug discovery and the heterogeneous abundance of CPP/AMP sequences among viral families opens new avenues in viral biology research.application/pdfpt_PTMining viral proteins for antimicrobial and cell-penetrating drug delivery peptidesFreire, João MiguelDias, Susana AlmeidaFlores, LuísVeiga, Ana SaloméCastanho, Miguel A. R. B.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1367-4803DOIIsPartOf10.1093/bioinformatics/btv1312016-03-18T15:01:49Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/23081http://purl.org/coar/access_right/c_16ecrestricted access196746 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/b14cba1a-6af9-4ffe-85cd-c74bc78ef930/downloadBioinformatics |
| spellingShingle | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides Freire, João Miguel |
| status | SINGLETON |
| title | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| title_full | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| title_fullStr | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| title_full_unstemmed | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| title_short | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| title_sort | Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides |
| url | http://hdl.handle.net/10451/23081 |
| visible | 1 |