Publicação
Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi
| Resumo: | Allene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses |
|---|---|
| Autores principais: | Serrazina, Susana |
| Outros Autores: | Machado, Helena; Costa, Rita Lourenço; Duque, Paula; Malhó, Rui |
| Assunto: | Arabidopsis thaliana allene oxide synthase plant defense gene functional analysis Phytophthora cinnamomi Castanea crenata Castanea sativa |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810984631369728 |
|---|---|
| author | Serrazina, Susana |
| author2 | Machado, Helena Costa, Rita Lourenço Duque, Paula Malhó, Rui |
| author2_role | author author author author |
| author_facet | Serrazina, Susana Machado, Helena Costa, Rita Lourenço Duque, Paula Malhó, Rui |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Serrazina, Susana\"},{\"Person.name\":\"Machado, Helena\"},{\"Person.name\":\"Costa, Rita Lourenço\"},{\"Person.name\":\"Duque, Paula\"},{\"Person.name\":\"Malhó, Rui\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Serrazina, Susana Machado, Helena Costa, Rita Lourenço Duque, Paula Malhó, Rui |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-05-28T10:11:34Z |
| datacite.date.embargoed.fl_str_mv | 2021-05-28T10:11:34Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Arabidopsis thaliana allene oxide synthase plant defense gene functional analysis Phytophthora cinnamomi Castanea crenata Castanea sativa |
| datacite.titles.title.fl_str_mv | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Serrazina, Susana Machado, Helena Costa, Rita Lourenço Duque, Paula Malhó, Rui |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-05-28T10:11:34Z |
| dc.date.embargoed.fl_str_mv | 2021-05-28T10:11:34Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/21375 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Frontiers in Plant Science |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Arabidopsis thaliana allene oxide synthase plant defense gene functional analysis Phytophthora cinnamomi Castanea crenata Castanea sativa |
| dc.title.fl_str_mv | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Allene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/071a2b89-84cb-425d-b0b6-8c8380edf9ce/download |
| id | ul_4046ecea60bf973b641eaee25d951fa0 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/21375 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/21375 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Serrazina, Susana Machado, Helena Costa, Rita Lourenço Duque, Paula Malhó, Rui |
| publishDate | 2021 |
| publisher.none.fl_str_mv | Frontiers in Plant Science |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engFrontiers in Plant Sciencept_PTAllene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responsesapplication/pdfpt_PTExpression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomiSerrazina, SusanaMachado, HelenaCosta, Rita LourençoDuque, PaulaMalhó, RuiHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.3389/fpls.2021.6286972021-05-28T10:11:34Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/21375http://purl.org/coar/access_right/c_abf2open accessArabidopsis thalianaallene oxide synthaseplant defensegene functional analysisPhytophthora cinnamomiCastanea crenataCastanea sativa5197416 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2021http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/071a2b89-84cb-425d-b0b6-8c8380edf9ce/downloadFrontiers in Plant Science |
| spellingShingle | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi Serrazina, Susana Arabidopsis thaliana allene oxide synthase plant defense gene functional analysis Phytophthora cinnamomi Castanea crenata Castanea sativa |
| status | SINGLETON |
| subject.fl_str_mv | Arabidopsis thaliana allene oxide synthase plant defense gene functional analysis Phytophthora cinnamomi Castanea crenata Castanea sativa |
| title | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| title_full | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| title_fullStr | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| title_full_unstemmed | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| title_short | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| title_sort | Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi |
| topic | Arabidopsis thaliana allene oxide synthase plant defense gene functional analysis Phytophthora cinnamomi Castanea crenata Castanea sativa |
| topic_facet | Arabidopsis thaliana allene oxide synthase plant defense gene functional analysis Phytophthora cinnamomi Castanea crenata Castanea sativa |
| url | http://hdl.handle.net/10400.5/21375 |
| visible | 1 |