Publicação
Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications
| Resumo: | Despite the high risk of extinction of the Iberian leuciscids, the impact of bacterial diseases on their conservation is unknown. A comprehensive knowledge of the interaction between these species and pathogens, such as mesophilic Aeromonas, as well as the development of biosafety measures for ex situ programs, are considered essential for the success of their conservation. The main objectives of this thesis are the evaluation of the epidemiology of mesophilic Aeromonas in Iberochondrostoma lusitanicum, as well as the characterization of their antimicrobial resistance and virulence profiles, the detection of differences in the diversity of Aeromonas spp. of I. lusitanicum and Squalius pyrenaicus, the evaluation of the potential of biosecurity measures in the control of Aeromonas spp. in ex situ programs and testing the effect of climate change on Aeromonas spp. The results showed that I. lusitanicum presented lower general health status in the dry season, varying with location. Aeromonas communities varied between seasons and locations, while their pathogenic potential increased in the dry season. Relevant resistance phenotypes (carbapenems and fluoroquinolones) were detected. Furthermore, I. lusitanicum and S. pyrenaicus showed different levels of skin lesions, with S. pyrenaicus being the most affected species. Aeromonas communities differed between the two fish species. Additionally, the use of biosecurity measures suggests a decrease in the prevalence of Aeromonas spp. in ex situ programs, as well as its pathogenicity to fish, while an increase in antimicrobial resistance was observed throughout the program. Finally, climate change predicted by the International Panel on Climate Change for water temperature and pH influenced the growth, biofilm production and antimicrobial resistance profiles of Aeromonas spp. The current results clarify the epidemiology of mesophilic Aeromonas in threatened Iberian leuciscids and contribute to the establishment of adequate conservation measures. |
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| Autores principais: | Grilo, Miguel |
| Assunto: | Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | tese de doutoramento |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| Resumo: | Despite the high risk of extinction of the Iberian leuciscids, the impact of bacterial diseases on their conservation is unknown. A comprehensive knowledge of the interaction between these species and pathogens, such as mesophilic Aeromonas, as well as the development of biosafety measures for ex situ programs, are considered essential for the success of their conservation. The main objectives of this thesis are the evaluation of the epidemiology of mesophilic Aeromonas in Iberochondrostoma lusitanicum, as well as the characterization of their antimicrobial resistance and virulence profiles, the detection of differences in the diversity of Aeromonas spp. of I. lusitanicum and Squalius pyrenaicus, the evaluation of the potential of biosecurity measures in the control of Aeromonas spp. in ex situ programs and testing the effect of climate change on Aeromonas spp. The results showed that I. lusitanicum presented lower general health status in the dry season, varying with location. Aeromonas communities varied between seasons and locations, while their pathogenic potential increased in the dry season. Relevant resistance phenotypes (carbapenems and fluoroquinolones) were detected. Furthermore, I. lusitanicum and S. pyrenaicus showed different levels of skin lesions, with S. pyrenaicus being the most affected species. Aeromonas communities differed between the two fish species. Additionally, the use of biosecurity measures suggests a decrease in the prevalence of Aeromonas spp. in ex situ programs, as well as its pathogenicity to fish, while an increase in antimicrobial resistance was observed throughout the program. Finally, climate change predicted by the International Panel on Climate Change for water temperature and pH influenced the growth, biofilm production and antimicrobial resistance profiles of Aeromonas spp. The current results clarify the epidemiology of mesophilic Aeromonas in threatened Iberian leuciscids and contribute to the establishment of adequate conservation measures. |
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