Publicação
Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications
| Resumo: | Despite the high risk of extinction of the Iberian leuciscids, the impact of bacterial diseases on their conservation is unknown. A comprehensive knowledge of the interaction between these species and pathogens, such as mesophilic Aeromonas, as well as the development of biosafety measures for ex situ programs, are considered essential for the success of their conservation. The main objectives of this thesis are the evaluation of the epidemiology of mesophilic Aeromonas in Iberochondrostoma lusitanicum, as well as the characterization of their antimicrobial resistance and virulence profiles, the detection of differences in the diversity of Aeromonas spp. of I. lusitanicum and Squalius pyrenaicus, the evaluation of the potential of biosecurity measures in the control of Aeromonas spp. in ex situ programs and testing the effect of climate change on Aeromonas spp. The results showed that I. lusitanicum presented lower general health status in the dry season, varying with location. Aeromonas communities varied between seasons and locations, while their pathogenic potential increased in the dry season. Relevant resistance phenotypes (carbapenems and fluoroquinolones) were detected. Furthermore, I. lusitanicum and S. pyrenaicus showed different levels of skin lesions, with S. pyrenaicus being the most affected species. Aeromonas communities differed between the two fish species. Additionally, the use of biosecurity measures suggests a decrease in the prevalence of Aeromonas spp. in ex situ programs, as well as its pathogenicity to fish, while an increase in antimicrobial resistance was observed throughout the program. Finally, climate change predicted by the International Panel on Climate Change for water temperature and pH influenced the growth, biofilm production and antimicrobial resistance profiles of Aeromonas spp. The current results clarify the epidemiology of mesophilic Aeromonas in threatened Iberian leuciscids and contribute to the establishment of adequate conservation measures. |
|---|---|
| Autores principais: | Grilo, Miguel |
| Assunto: | Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | tese de doutoramento |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866809752134090752 |
|---|---|
| author | Grilo, Miguel |
| author_facet | Grilo, Miguel |
| author_role | author |
| contributor_name_str_mv | Oliveira, Maria Manuela Castilho Monteiro de Robalo, Joana Isabel Espírito Santo Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Grilo, Miguel\",\"Person.identifier.orcid\":\"0000-0001-7364-821X\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Oliveira, Maria Manuela Castilho Monteiro de Robalo, Joana Isabel Espírito Santo Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Grilo, Miguel |
| datacite.date.Accepted.fl_str_mv | 2022-02-17T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-03-24T22:45:36Z |
| datacite.date.embargoed.fl_str_mv | 2022-03-24T22:45:36Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| datacite.titles.title.fl_str_mv | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| dc.contributor.none.fl_str_mv | Oliveira, Maria Manuela Castilho Monteiro de Robalo, Joana Isabel Espírito Santo Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Grilo, Miguel |
| dc.date.Accepted.fl_str_mv | 2022-02-17T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-03-24T22:45:36Z |
| dc.date.embargoed.fl_str_mv | 2022-03-24T22:45:36Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/23902 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Universidade de Lisboa, Faculdade de Medicina Veterinária |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| dc.title.fl_str_mv | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_db06 |
| description | Despite the high risk of extinction of the Iberian leuciscids, the impact of bacterial diseases on their conservation is unknown. A comprehensive knowledge of the interaction between these species and pathogens, such as mesophilic Aeromonas, as well as the development of biosafety measures for ex situ programs, are considered essential for the success of their conservation. The main objectives of this thesis are the evaluation of the epidemiology of mesophilic Aeromonas in Iberochondrostoma lusitanicum, as well as the characterization of their antimicrobial resistance and virulence profiles, the detection of differences in the diversity of Aeromonas spp. of I. lusitanicum and Squalius pyrenaicus, the evaluation of the potential of biosecurity measures in the control of Aeromonas spp. in ex situ programs and testing the effect of climate change on Aeromonas spp. The results showed that I. lusitanicum presented lower general health status in the dry season, varying with location. Aeromonas communities varied between seasons and locations, while their pathogenic potential increased in the dry season. Relevant resistance phenotypes (carbapenems and fluoroquinolones) were detected. Furthermore, I. lusitanicum and S. pyrenaicus showed different levels of skin lesions, with S. pyrenaicus being the most affected species. Aeromonas communities differed between the two fish species. Additionally, the use of biosecurity measures suggests a decrease in the prevalence of Aeromonas spp. in ex situ programs, as well as its pathogenicity to fish, while an increase in antimicrobial resistance was observed throughout the program. Finally, climate change predicted by the International Panel on Climate Change for water temperature and pH influenced the growth, biofilm production and antimicrobial resistance profiles of Aeromonas spp. The current results clarify the epidemiology of mesophilic Aeromonas in threatened Iberian leuciscids and contribute to the establishment of adequate conservation measures. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | doctoralThesis |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/f1329699-196e-40ba-b93f-c1a25d79cfc7/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ul_46bfce9fce42e67592ed4e30a5a3e71e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/23902 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/23902 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Grilo, Miguel Grilo, Miguel https://www.ciencia-id.pt/4B17-DF6F-1A51 4B17-DF6F-1A51 http://orcid.org/0000-0001-7364-821X 0000-0001-7364-821X |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Universidade de Lisboa, Faculdade de Medicina Veterinária |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engUniversidade de Lisboa, Faculdade de Medicina Veterináriapt_PTDespite the high risk of extinction of the Iberian leuciscids, the impact of bacterial diseases on their conservation is unknown. A comprehensive knowledge of the interaction between these species and pathogens, such as mesophilic Aeromonas, as well as the development of biosafety measures for ex situ programs, are considered essential for the success of their conservation. The main objectives of this thesis are the evaluation of the epidemiology of mesophilic Aeromonas in Iberochondrostoma lusitanicum, as well as the characterization of their antimicrobial resistance and virulence profiles, the detection of differences in the diversity of Aeromonas spp. of I. lusitanicum and Squalius pyrenaicus, the evaluation of the potential of biosecurity measures in the control of Aeromonas spp. in ex situ programs and testing the effect of climate change on Aeromonas spp. The results showed that I. lusitanicum presented lower general health status in the dry season, varying with location. Aeromonas communities varied between seasons and locations, while their pathogenic potential increased in the dry season. Relevant resistance phenotypes (carbapenems and fluoroquinolones) were detected. Furthermore, I. lusitanicum and S. pyrenaicus showed different levels of skin lesions, with S. pyrenaicus being the most affected species. Aeromonas communities differed between the two fish species. Additionally, the use of biosecurity measures suggests a decrease in the prevalence of Aeromonas spp. in ex situ programs, as well as its pathogenicity to fish, while an increase in antimicrobial resistance was observed throughout the program. Finally, climate change predicted by the International Panel on Climate Change for water temperature and pH influenced the growth, biofilm production and antimicrobial resistance profiles of Aeromonas spp. The current results clarify the epidemiology of mesophilic Aeromonas in threatened Iberian leuciscids and contribute to the establishment of adequate conservation measures.application/pdfpt_PTMesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implicationsPersonalGrilo, MiguelDSpacehttp://dspace.org/items/1f136cc1-1f56-40b3-971b-aab3edaa7cabDSpacehttp://dspace.org/items/1f136cc1-1f56-40b3-971b-aab3edaa7cabGriloMiguelCiência IDhttps://www.ciencia-id.pt4B17-DF6F-1A51ORCIDhttp://orcid.org0000-0001-7364-821XScopus Author IDhttps://www.scopus.com57195729611Oliveira, Maria Manuela Castilho Monteiro deRobalo, Joana Isabel Espírito SantoHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptURNurn:tid:1015527422022-03-24T22:45:36Z2022-02-172022-02-17T00:00:00ZHandlehttp://hdl.handle.net/10400.5/23902http://purl.org/coar/access_right/c_abf2open accessAeromonas spp.Iberochondrostoma lusitanicumSqualius pyrenaicusAntimicrobial resistanceVirulenceResistência antimicrobianaVirulência10769856 bytesFundação para a Ciência e a TecnologiaCentre for Interdisciplinary Research in Animal Health6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_db06doctoral thesis2022-02-17http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/f1329699-196e-40ba-b93f-c1a25d79cfc7/download |
| spellingShingle | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications Grilo, Miguel Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| status | SINGLETON |
| subject.fl_str_mv | Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| title | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| title_full | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| title_fullStr | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| title_full_unstemmed | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| title_short | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| title_sort | Mesophilic Aeromonas in threatened Iberian leuciscids : conservation and public health implications |
| topic | Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| topic_facet | Aeromonas spp. Iberochondrostoma lusitanicum Squalius pyrenaicus Antimicrobial resistance Virulence Resistência antimicrobiana Virulência |
| url | http://hdl.handle.net/10400.5/23902 |
| visible | 1 |