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Different ecophysiological responses of freshwater fish to warming and acidification

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Resumo:Future climate change scenarios predict threatening outcomes to biodiversity. Available empirical data concerning biological response of freshwater fish to climate change remains scarce. In this study, we investigated the physiological and biochemical responses of two Iberian freshwater fish species (Squalius carolitertii and the endangered S. torgalensis), inhabiting different climatic conditions, to projected future scenarios of warming (+3°C) and acidification (ΔpH=-0.4). Herein, metabolic enzyme activities of glycolytic (citrate synthase - CS, lactate dehydrogenase - LDH) and antioxidant (glutathione S-transferase, catalase and superoxide dismutase) pathways, as well as the heat shock response (HSR) and lipid peroxidation were determined. Our results show that, under current water pH, warming causes differential interspecific changes on LDH activity, increasing and decreasing its activity in S. carolitertii and in S. torgalensis, respectively. Furthermore, the synergistic effect of warming and acidification caused an increase in LDH activity of S. torgalensis, comparing with the warming condition. As for CS activity, acidification significantly decreased its activity in S. carolitertii whereas in S. torgalensis no significant effect was observed. These results suggest that S. carolitertii is more vulnerable to climate change, possibly as the result of its evolutionary acclimatization to milder climatic condition, while S. torgalensis evolved in the warmer Mediterranean climate. However, significant changes in HSR were observed under the combined warming and acidification (S. carolitertii) or under acidification (S. torgalensis). Our results underlie the importance of conducting experimental studies and address species endpoint responses under projected climate change scenarios to improve conservation strategies, and to safeguard endangered freshwater fish.
Autores principais:Jesus, Tiago F.
Outros Autores:Rosa, Inês C.; Repolho, Tiago; Lopes, Ana Rita; Pimentel, Marta; Almeida-Val, Vera M.F.; Coelho, Maria M.; Rosa, Rui
Assunto:Acidification Oxidative stress Heat shock response Squalius carolitertii Squalius torgalensis Warming
Ano:2018
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa
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author Jesus, Tiago F.
author2 Rosa, Inês C.
Repolho, Tiago
Lopes, Ana Rita
Pimentel, Marta
Almeida-Val, Vera M.F.
Coelho, Maria M.
Rosa, Rui
author2_role author
author
author
author
author
author
author
author_facet Jesus, Tiago F.
Rosa, Inês C.
Repolho, Tiago
Lopes, Ana Rita
Pimentel, Marta
Almeida-Val, Vera M.F.
Coelho, Maria M.
Rosa, Rui
author_role author
contributor_name_str_mv Repositório Científico de Acesso Aberto da ULisboa
country_str PT
creators_json_txt [{\"Person.name\":\"Jesus, Tiago F.\"},{\"Person.name\":\"Rosa, Inês C.\",\"Person.identifier.orcid\":\"0000-0001-9530-1057\"},{\"Person.name\":\"Repolho, Tiago\",\"Person.identifier.orcid\":\"0000-0002-1048-8009\"},{\"Person.name\":\"Lopes, Ana Rita\",\"Person.identifier.orcid\":\"0000-0002-3992-0715\"},{\"Person.name\":\"Pimentel, Marta\",\"Person.identifier.orcid\":\"0000-0003-3248-4479\"},{\"Person.name\":\"Almeida-Val, Vera M.F.\"},{\"Person.name\":\"Coelho, Maria M.\"},{\"Person.name\":\"Rosa, Rui\",\"Person.identifier.orcid\":\"0000-0003-2801-5178\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
datacite.creators.creator.creatorName.fl_str_mv Jesus, Tiago F.
Rosa, Inês C.
Repolho, Tiago
Lopes, Ana Rita
Pimentel, Marta
Almeida-Val, Vera M.F.
Coelho, Maria M.
Rosa, Rui
datacite.date.Accepted.fl_str_mv 2018-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2020-01-19T20:47:03Z
datacite.date.embargoed.fl_str_mv 2020-01-19T20:47:03Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv Acidification
Oxidative stress
Heat shock response
Squalius carolitertii
Squalius torgalensis
Warming
datacite.titles.title.fl_str_mv Different ecophysiological responses of freshwater fish to warming and acidification
dc.contributor.none.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
dc.creator.none.fl_str_mv Jesus, Tiago F.
Rosa, Inês C.
Repolho, Tiago
Lopes, Ana Rita
Pimentel, Marta
Almeida-Val, Vera M.F.
Coelho, Maria M.
Rosa, Rui
dc.date.Accepted.fl_str_mv 2018-01-01T00:00:00Z
dc.date.available.fl_str_mv 2020-01-19T20:47:03Z
dc.date.embargoed.fl_str_mv 2020-01-19T20:47:03Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10451/41206
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Elsevier
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv Acidification
Oxidative stress
Heat shock response
Squalius carolitertii
Squalius torgalensis
Warming
dc.title.fl_str_mv Different ecophysiological responses of freshwater fish to warming and acidification
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Future climate change scenarios predict threatening outcomes to biodiversity. Available empirical data concerning biological response of freshwater fish to climate change remains scarce. In this study, we investigated the physiological and biochemical responses of two Iberian freshwater fish species (Squalius carolitertii and the endangered S. torgalensis), inhabiting different climatic conditions, to projected future scenarios of warming (+3°C) and acidification (ΔpH=-0.4). Herein, metabolic enzyme activities of glycolytic (citrate synthase - CS, lactate dehydrogenase - LDH) and antioxidant (glutathione S-transferase, catalase and superoxide dismutase) pathways, as well as the heat shock response (HSR) and lipid peroxidation were determined. Our results show that, under current water pH, warming causes differential interspecific changes on LDH activity, increasing and decreasing its activity in S. carolitertii and in S. torgalensis, respectively. Furthermore, the synergistic effect of warming and acidification caused an increase in LDH activity of S. torgalensis, comparing with the warming condition. As for CS activity, acidification significantly decreased its activity in S. carolitertii whereas in S. torgalensis no significant effect was observed. These results suggest that S. carolitertii is more vulnerable to climate change, possibly as the result of its evolutionary acclimatization to milder climatic condition, while S. torgalensis evolved in the warmer Mediterranean climate. However, significant changes in HSR were observed under the combined warming and acidification (S. carolitertii) or under acidification (S. torgalensis). Our results underlie the importance of conducting experimental studies and address species endpoint responses under projected climate change scenarios to improve conservation strategies, and to safeguard endangered freshwater fish.
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eu_rights_str_mv restrictedAccess
format article
fulltext.url.fl_str_mv https://repositorio.ulisboa.pt/bitstreams/8aa030a0-db2a-41a5-8d30-ca608eef026c/download
id ul_4e5a6701b8f5532295ff94eed6b09fee
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institution Universidade de Lisboa
instname_str Universidade de Lisboa
language eng
network_acronym_str ul
network_name_str Repositório da Universidade de Lisboa
oai_identifier_str oai:repositorio.ulisboa.pt:10451/41206
organization_str_mv urn:organizationAcronym:ul
person_str_mv Jesus, Tiago F.
Rosa, Inês C.
Rosa, Inês C.
https://www.ciencia-id.pt/9815-98C7-423E
9815-98C7-423E
http://orcid.org/0000-0001-9530-1057
0000-0001-9530-1057
Repolho, Tiago
Repolho, Tiago
https://www.ciencia-id.pt/7812-9026-CAC2
7812-9026-CAC2
http://orcid.org/0000-0002-1048-8009
0000-0002-1048-8009
Lopes, Ana Rita
Lopes, Ana Rita
https://www.ciencia-id.pt/611A-674E-B9CD
611A-674E-B9CD
http://orcid.org/0000-0002-3992-0715
0000-0002-3992-0715
Pimentel, Marta
Pimentel, Marta
https://www.ciencia-id.pt/8C1F-E4AF-FA3B
8C1F-E4AF-FA3B
http://orcid.org/0000-0003-3248-4479
0000-0003-3248-4479
Almeida-Val, Vera M.F.
Coelho, Maria M.
Rosa, Rui
Rosa, Rui
https://www.ciencia-id.pt/2B10-7D61-FF7A
2B10-7D61-FF7A
http://orcid.org/0000-0003-2801-5178
0000-0003-2801-5178
publishDate 2018
publisher.none.fl_str_mv Elsevier
reponame_str Repositório da Universidade de Lisboa
repository_id_str urn:repositoryAcronym:ul
service_str_mv urn:repositoryAcronym:ul
spelling engElsevierpt_PTFuture climate change scenarios predict threatening outcomes to biodiversity. Available empirical data concerning biological response of freshwater fish to climate change remains scarce. In this study, we investigated the physiological and biochemical responses of two Iberian freshwater fish species (Squalius carolitertii and the endangered S. torgalensis), inhabiting different climatic conditions, to projected future scenarios of warming (+3°C) and acidification (ΔpH=-0.4). Herein, metabolic enzyme activities of glycolytic (citrate synthase - CS, lactate dehydrogenase - LDH) and antioxidant (glutathione S-transferase, catalase and superoxide dismutase) pathways, as well as the heat shock response (HSR) and lipid peroxidation were determined. Our results show that, under current water pH, warming causes differential interspecific changes on LDH activity, increasing and decreasing its activity in S. carolitertii and in S. torgalensis, respectively. Furthermore, the synergistic effect of warming and acidification caused an increase in LDH activity of S. torgalensis, comparing with the warming condition. As for CS activity, acidification significantly decreased its activity in S. carolitertii whereas in S. torgalensis no significant effect was observed. These results suggest that S. carolitertii is more vulnerable to climate change, possibly as the result of its evolutionary acclimatization to milder climatic condition, while S. torgalensis evolved in the warmer Mediterranean climate. However, significant changes in HSR were observed under the combined warming and acidification (S. carolitertii) or under acidification (S. torgalensis). Our results underlie the importance of conducting experimental studies and address species endpoint responses under projected climate change scenarios to improve conservation strategies, and to safeguard endangered freshwater fish.application/pdfpt_PTDifferent ecophysiological responses of freshwater fish to warming and acidificationJesus, Tiago F.PersonalRosa, Inês C.DSpacehttp://dspace.org/items/e8e5a1cb-edbc-4527-859c-42c801ec8715DSpacehttp://dspace.org/items/e8e5a1cb-edbc-4527-859c-42c801ec8715RosaInêsCiência IDhttps://www.ciencia-id.pt9815-98C7-423EORCIDhttp://orcid.org0000-0001-9530-1057Researcher IDhttps://www.researcherid.comI-5925-2014Scopus Author IDhttps://www.scopus.com43861770000PersonalRepolho, TiagoDSpacehttp://dspace.org/items/0374386d-3719-4cf4-b44b-09c34fa94a1dDSpacehttp://dspace.org/items/0374386d-3719-4cf4-b44b-09c34fa94a1dRepolhoTiagoCiência IDhttps://www.ciencia-id.pt7812-9026-CAC2ORCIDhttp://orcid.org0000-0002-1048-8009Scopus Author IDhttps://www.scopus.com18435367300PersonalLopes, Ana RitaDSpacehttp://dspace.org/items/70f0a966-815d-45c5-9540-ad733c62d223DSpacehttp://dspace.org/items/70f0a966-815d-45c5-9540-ad733c62d223LopesAna RitaCiência IDhttps://www.ciencia-id.pt611A-674E-B9CDORCIDhttp://orcid.org0000-0002-3992-0715Scopus Author IDhttps://www.scopus.com55895278700PersonalPimentel, MartaDSpacehttp://dspace.org/items/2b44fb45-83f0-42e3-8cb3-1bdf3cbfebc0DSpacehttp://dspace.org/items/2b44fb45-83f0-42e3-8cb3-1bdf3cbfebc0Silva Pimentel da SilvaMarta CristinaCiência IDhttps://www.ciencia-id.pt8C1F-E4AF-FA3BORCIDhttp://orcid.org0000-0003-3248-4479Almeida-Val, Vera M.F.Coelho, Maria M.PersonalRosa, RuiDSpacehttp://dspace.org/items/395f77c0-ac42-4b7c-9b4a-07fdb75305ccDSpacehttp://dspace.org/items/395f77c0-ac42-4b7c-9b4a-07fdb75305ccRosaRuiCiência IDhttps://www.ciencia-id.pt2B10-7D61-FF7AORCIDhttp://orcid.org0000-0003-2801-5178Researcher IDhttps://www.researcherid.comA-4580-2009Scopus Author IDhttps://www.scopus.com7102610088HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1095-6433/DOIIsPartOf10.1016/j.cbpa.2017.11.0072020-01-19T20:47:03Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/41206http://purl.org/coar/access_right/c_16ecrestricted accessAcidificationOxidative stressHeat shock responseSqualius carolitertiiSqualius torgalensisWarming884642 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/8aa030a0-db2a-41a5-8d30-ca608eef026c/downloadComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology2163441
spellingShingle Different ecophysiological responses of freshwater fish to warming and acidification
Jesus, Tiago F.
Acidification
Oxidative stress
Heat shock response
Squalius carolitertii
Squalius torgalensis
Warming
status SINGLETON
subject.fl_str_mv Acidification
Oxidative stress
Heat shock response
Squalius carolitertii
Squalius torgalensis
Warming
title Different ecophysiological responses of freshwater fish to warming and acidification
title_full Different ecophysiological responses of freshwater fish to warming and acidification
title_fullStr Different ecophysiological responses of freshwater fish to warming and acidification
title_full_unstemmed Different ecophysiological responses of freshwater fish to warming and acidification
title_short Different ecophysiological responses of freshwater fish to warming and acidification
title_sort Different ecophysiological responses of freshwater fish to warming and acidification
topic Acidification
Oxidative stress
Heat shock response
Squalius carolitertii
Squalius torgalensis
Warming
topic_facet Acidification
Oxidative stress
Heat shock response
Squalius carolitertii
Squalius torgalensis
Warming
url http://hdl.handle.net/10451/41206
visible 1