Publicação
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora
| Resumo: | Understanding the effect of extreme temperatures and elevated air (CO2) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid C. canephora and its polyploid C. arabica, grown at 25 C and at two supra-optimal temperatures (37 C, 42 C), under ambient (aCO2) or elevated air CO2 (eCO2). Both species expressed fewer genes as temperature rose, although a high number of differentially expressed genes (DEGs) were observed, especially at 42 C. An enrichment analysis revealed that the two species reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic machinery until 37 C. Although eCO2 helped to release stress, 42 C had a severe impact on both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high temperatures and eCO2, which may be used as key probe genes in future studies. This was mostly felt in C. arabica, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity, chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated, especially under 42 C, regardless of CO2. Transcriptomic changes showed that both species were strongly affected by the highest temperature, although they can endure higher temperatures (37 C) than previously assumed. |
|---|---|
| Autores principais: | Marques, Isabel |
| Outros Autores: | Fernandes, Isabel; S. Paulo, Octávio; Lidon, Fernando C.; DaMatta, Fábio M.; Ramalho, José C.; Ribeiro-Barros, Ana I. |
| Assunto: | climate changes coffee elevated air (CO2) functional analysis high temperatures leaf RNAseq polyploidy warming |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866811558482411520 |
|---|---|
| author | Marques, Isabel |
| author2 | Fernandes, Isabel S. Paulo, Octávio Lidon, Fernando C. DaMatta, Fábio M. Ramalho, José C. Ribeiro-Barros, Ana I. |
| author2_role | author author author author author author |
| author_facet | Marques, Isabel Fernandes, Isabel S. Paulo, Octávio Lidon, Fernando C. DaMatta, Fábio M. Ramalho, José C. Ribeiro-Barros, Ana I. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Marques, Isabel\",\"Person.identifier.orcid\":\"0000-0001-9788-4831\"},{\"Person.name\":\"Fernandes, Isabel\",\"Person.identifier.orcid\":\"0000-0003-1485-5942\"},{\"Person.name\":\"S. Paulo, Octávio\",\"Person.identifier.orcid\":\"0000-0001-5408-5212\"},{\"Person.name\":\"Lidon, Fernando C.\"},{\"Person.name\":\"DaMatta, Fábio M.\"},{\"Person.name\":\"Ramalho, José C.\"},{\"Person.name\":\"Ribeiro-Barros, Ana I.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Marques, Isabel Fernandes, Isabel S. Paulo, Octávio Lidon, Fernando C. DaMatta, Fábio M. Ramalho, José C. Ribeiro-Barros, Ana I. |
| datacite.date.Accepted.fl_str_mv | 2021-03-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-11-17T19:53:00Z |
| datacite.date.embargoed.fl_str_mv | 2021-11-17T19:53:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | climate changes coffee elevated air (CO2) functional analysis high temperatures leaf RNAseq polyploidy warming |
| datacite.titles.title.fl_str_mv | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Marques, Isabel Fernandes, Isabel S. Paulo, Octávio Lidon, Fernando C. DaMatta, Fábio M. Ramalho, José C. Ribeiro-Barros, Ana I. |
| dc.date.Accepted.fl_str_mv | 2021-03-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-11-17T19:53:00Z |
| dc.date.embargoed.fl_str_mv | 2021-11-17T19:53:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/50137 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | climate changes coffee elevated air (CO2) functional analysis high temperatures leaf RNAseq polyploidy warming |
| dc.title.fl_str_mv | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Understanding the effect of extreme temperatures and elevated air (CO2) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid C. canephora and its polyploid C. arabica, grown at 25 C and at two supra-optimal temperatures (37 C, 42 C), under ambient (aCO2) or elevated air CO2 (eCO2). Both species expressed fewer genes as temperature rose, although a high number of differentially expressed genes (DEGs) were observed, especially at 42 C. An enrichment analysis revealed that the two species reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic machinery until 37 C. Although eCO2 helped to release stress, 42 C had a severe impact on both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high temperatures and eCO2, which may be used as key probe genes in future studies. This was mostly felt in C. arabica, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity, chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated, especially under 42 C, regardless of CO2. Transcriptomic changes showed that both species were strongly affected by the highest temperature, although they can endure higher temperatures (37 C) than previously assumed. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/ba749665-1e7b-41e5-adf7-1c9e7af65012/download |
| funding.funder.alternateName_str_mv | EC |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100008530 |
| funding.funder.name_str_mv | European Commission |
| funding.name_str_mv | H2020 |
| id | ul_6fc4c93aaa9bca7e2ef70e3200b6ddf2 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/50137 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/50137 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Marques, Isabel Marques, Isabel https://www.ciencia-id.pt/A71A-611E-44B3 A71A-611E-44B3 http://orcid.org/0000-0001-9788-4831 0000-0001-9788-4831 Fernandes, Isabel Fernandes, Isabel https://www.ciencia-id.pt/0F13-E4FD-F6AF 0F13-E4FD-F6AF http://orcid.org/0000-0003-1485-5942 0000-0003-1485-5942 S. Paulo, Octávio S. Paulo, Octávio https://www.ciencia-id.pt/471A-1219-9C5F 471A-1219-9C5F http://orcid.org/0000-0001-5408-5212 0000-0001-5408-5212 Lidon, Fernando C. DaMatta, Fábio M. Ramalho, José C. Ribeiro-Barros, Ana I. |
| publishDate | 2021 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMDPIpt_PTUnderstanding the effect of extreme temperatures and elevated air (CO2) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid C. canephora and its polyploid C. arabica, grown at 25 C and at two supra-optimal temperatures (37 C, 42 C), under ambient (aCO2) or elevated air CO2 (eCO2). Both species expressed fewer genes as temperature rose, although a high number of differentially expressed genes (DEGs) were observed, especially at 42 C. An enrichment analysis revealed that the two species reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic machinery until 37 C. Although eCO2 helped to release stress, 42 C had a severe impact on both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high temperatures and eCO2, which may be used as key probe genes in future studies. This was mostly felt in C. arabica, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity, chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated, especially under 42 C, regardless of CO2. Transcriptomic changes showed that both species were strongly affected by the highest temperature, although they can endure higher temperatures (37 C) than previously assumed.application/pdfpt_PTA Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephoraPersonalMarques, IsabelDSpacehttp://dspace.org/items/554ac833-5950-4e34-8e69-cb36c20e8847DSpacehttp://dspace.org/items/554ac833-5950-4e34-8e69-cb36c20e8847Dos Santos Lourenço MarquesIsabel CristinaCiência IDhttps://www.ciencia-id.ptA71A-611E-44B3ORCIDhttp://orcid.org0000-0001-9788-4831Scopus Author IDhttps://www.scopus.com19337527300PersonalFernandes, IsabelDSpacehttp://dspace.org/items/1e007254-1e82-4949-8743-758928fcb53dDSpacehttp://dspace.org/items/1e007254-1e82-4949-8743-758928fcb53dFernandesIsabelCiência IDhttps://www.ciencia-id.pt0F13-E4FD-F6AFORCIDhttp://orcid.org0000-0003-1485-5942PersonalS. Paulo, OctávioDSpacehttp://dspace.org/items/2be59178-7c2d-4df6-9d6f-3aaef29c0a53DSpacehttp://dspace.org/items/2be59178-7c2d-4df6-9d6f-3aaef29c0a53PauloOctávioCiência IDhttps://www.ciencia-id.pt471A-1219-9C5FORCIDhttp://orcid.org0000-0001-5408-5212Researcher IDhttps://www.researcherid.comD-9921-2011Scopus Author IDhttps://www.scopus.com6602677193Lidon, Fernando C.DaMatta, Fábio M.Ramalho, José C.Ribeiro-Barros, Ana I.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1422-0067DOIIsPartOf10.3390/ijms220631252021-11-17T19:53:00Z2021-032021-03-01T00:00:00ZHandlehttp://hdl.handle.net/10451/50137http://purl.org/coar/access_right/c_abf2open accessclimate changescoffeeelevated air (CO2)functional analysishigh temperaturesleaf RNAseqpolyploidywarming4436709 bytesEuropean CommissionBREEDing Coffee for AgroForestry SystemsH2020Crossref Funder IDhttp://doi.org/10.13039/501100008530literaturehttp://purl.org/coar/resource_type/c_6501journal article2021-03http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/ba749665-1e7b-41e5-adf7-1c9e7af65012/downloadInternational Journal of Molecular Sciences2263125 |
| spellingShingle | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora Marques, Isabel climate changes coffee elevated air (CO2) functional analysis high temperatures leaf RNAseq polyploidy warming |
| status | SINGLETON |
| subject.fl_str_mv | climate changes coffee elevated air (CO2) functional analysis high temperatures leaf RNAseq polyploidy warming |
| title | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| title_full | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| title_fullStr | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| title_full_unstemmed | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| title_short | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| title_sort | A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora |
| topic | climate changes coffee elevated air (CO2) functional analysis high temperatures leaf RNAseq polyploidy warming |
| topic_facet | climate changes coffee elevated air (CO2) functional analysis high temperatures leaf RNAseq polyploidy warming |
| url | http://hdl.handle.net/10451/50137 |
| visible | 1 |