Publicação
Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
| Resumo: | The objective of the present work was to study the effects of erythrocyte proteins phosphorylation in erythrocyte aggregation and deformability. Human whole blood samples were incubated in vitro in absent and in presence of the phosphorylation/dephosphorilation band 3 inhibitors and also with adenylyl cyclase, guanylate cyclase and PI3K inhibitors and the erythrocyte aggregation index (EAI) and deformability were assayed. The results show that when band 3 is phosphorylated in presence of a PTP inhibitor an increase in erythrocyte aggregation index is observed (p < 0.0001). A partial dephosphorylation band 3 state, induced by PTK inhibitors, show a decrease in the erythrocyte aggregation index (p < 0.002). However both manipulated states induced lower EAI values than blood samples aliquots controls. The guanylate cyclase and PI3-K inhibitors significantly decrease the erythrocyte aggregation index in relation with the control blood samples. Erythrocyte deformability in presence of all the inhibitors did not showed significant changes. PTP and PI3-K inhibitors showed a significantly increase in the plasma potassium concentrations not associated with EAI values. Methehemoglobin levels were increased significantly when guanylate cyclase inhibitor is present in the blood samples. In conclusion, the results suggest that erythrocyte aggregation index is dependent of the phosphorylated/dephosphorylated state of band 3. |
|---|---|
| Autores principais: | Saldanha, Carlota |
| Outros Autores: | Silva, Ana S.; Gonçalves, Sónia; Martins-Silva, João |
| Assunto: | Band 3 Erythrocyte Phosphorylation Dephosphorylation Protein tyrosine kinase Protein tyrosine phosphatase |
| Ano: | 2007 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866811258255179776 |
|---|---|
| author | Saldanha, Carlota |
| author2 | Silva, Ana S. Gonçalves, Sónia Martins-Silva, João |
| author2_role | author author author |
| author_facet | Saldanha, Carlota Silva, Ana S. Gonçalves, Sónia Martins-Silva, João |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Saldanha, Carlota\"},{\"Person.name\":\"Silva, Ana S.\"},{\"Person.name\":\"Gonçalves, Sónia\"},{\"Person.name\":\"Martins-Silva, João\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Saldanha, Carlota Silva, Ana S. Gonçalves, Sónia Martins-Silva, João |
| datacite.date.Accepted.fl_str_mv | 2007-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-10-01T15:48:52Z |
| datacite.date.embargoed.fl_str_mv | 2015-10-01T15:48:52Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Band 3 Erythrocyte Phosphorylation Dephosphorylation Protein tyrosine kinase Protein tyrosine phosphatase |
| datacite.titles.title.fl_str_mv | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Saldanha, Carlota Silva, Ana S. Gonçalves, Sónia Martins-Silva, João |
| dc.date.Accepted.fl_str_mv | 2007-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-10-01T15:48:52Z |
| dc.date.embargoed.fl_str_mv | 2015-10-01T15:48:52Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch969 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | IOS Press |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Band 3 Erythrocyte Phosphorylation Dephosphorylation Protein tyrosine kinase Protein tyrosine phosphatase |
| dc.title.fl_str_mv | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The objective of the present work was to study the effects of erythrocyte proteins phosphorylation in erythrocyte aggregation and deformability. Human whole blood samples were incubated in vitro in absent and in presence of the phosphorylation/dephosphorilation band 3 inhibitors and also with adenylyl cyclase, guanylate cyclase and PI3K inhibitors and the erythrocyte aggregation index (EAI) and deformability were assayed. The results show that when band 3 is phosphorylated in presence of a PTP inhibitor an increase in erythrocyte aggregation index is observed (p < 0.0001). A partial dephosphorylation band 3 state, induced by PTK inhibitors, show a decrease in the erythrocyte aggregation index (p < 0.002). However both manipulated states induced lower EAI values than blood samples aliquots controls. The guanylate cyclase and PI3-K inhibitors significantly decrease the erythrocyte aggregation index in relation with the control blood samples. Erythrocyte deformability in presence of all the inhibitors did not showed significant changes. PTP and PI3-K inhibitors showed a significantly increase in the plasma potassium concentrations not associated with EAI values. Methehemoglobin levels were increased significantly when guanylate cyclase inhibitor is present in the blood samples. In conclusion, the results suggest that erythrocyte aggregation index is dependent of the phosphorylated/dephosphorylated state of band 3. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/02a7dc8b-4385-4afe-8f63-c6ea902a8237/download |
| id | ul_72e9dcdbe54fb4e905fc3d96b8a12de2 |
| identifier.url.fl_str_mv | http://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch969 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/20193 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Saldanha, Carlota Silva, Ana S. Gonçalves, Sónia Martins-Silva, João |
| publishDate | 2007 |
| publisher.none.fl_str_mv | IOS Press |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engIOS Pressen_ENThe objective of the present work was to study the effects of erythrocyte proteins phosphorylation in erythrocyte aggregation and deformability. Human whole blood samples were incubated in vitro in absent and in presence of the phosphorylation/dephosphorilation band 3 inhibitors and also with adenylyl cyclase, guanylate cyclase and PI3K inhibitors and the erythrocyte aggregation index (EAI) and deformability were assayed. The results show that when band 3 is phosphorylated in presence of a PTP inhibitor an increase in erythrocyte aggregation index is observed (p < 0.0001). A partial dephosphorylation band 3 state, induced by PTK inhibitors, show a decrease in the erythrocyte aggregation index (p < 0.002). However both manipulated states induced lower EAI values than blood samples aliquots controls. The guanylate cyclase and PI3-K inhibitors significantly decrease the erythrocyte aggregation index in relation with the control blood samples. Erythrocyte deformability in presence of all the inhibitors did not showed significant changes. PTP and PI3-K inhibitors showed a significantly increase in the plasma potassium concentrations not associated with EAI values. Methehemoglobin levels were increased significantly when guanylate cyclase inhibitor is present in the blood samples. In conclusion, the results suggest that erythrocyte aggregation index is dependent of the phosphorylated/dephosphorylated state of band 3.application/pdfen_ENModulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylationSaldanha, CarlotaSilva, Ana S.Gonçalves, SóniaMartins-Silva, JoãoHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptHandlehttp://hdl.handle.net/10451/20193ISSNIsPartOf1386-02912015-10-01T15:48:52Z20072007-01-01T00:00:00ZURLhttp://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch969http://purl.org/coar/access_right/c_abf2open accessBand 3ErythrocytePhosphorylationDephosphorylationProtein tyrosine kinaseProtein tyrosine phosphatase198673 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/02a7dc8b-4385-4afe-8f63-c6ea902a8237/downloadClinical Hemorheology and Microcirculation |
| spellingShingle | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation Saldanha, Carlota Band 3 Erythrocyte Phosphorylation Dephosphorylation Protein tyrosine kinase Protein tyrosine phosphatase |
| status | SINGLETON |
| subject.fl_str_mv | Band 3 Erythrocyte Phosphorylation Dephosphorylation Protein tyrosine kinase Protein tyrosine phosphatase |
| title | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| title_full | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| title_fullStr | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| title_full_unstemmed | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| title_short | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| title_sort | Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation |
| topic | Band 3 Erythrocyte Phosphorylation Dephosphorylation Protein tyrosine kinase Protein tyrosine phosphatase |
| topic_facet | Band 3 Erythrocyte Phosphorylation Dephosphorylation Protein tyrosine kinase Protein tyrosine phosphatase |
| url | http://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch969 |
| visible | 1 |